Team:HIT-Harbin/Judging

From 2014.igem.org

(Difference between revisions)
(Replaced content with "{{CSS/Main}} <html> <script>toolbar('nav')</script>")
 
(5 intermediate revisions not shown)
Line 2: Line 2:
<html>
<html>
-
<link type="text/css" rel="stylesheet" media="screen" href="https://2014.igem.org/Team:HIT-Harbin/css/style.css?action=raw&amp;ctype=text/css">
 
-
<link type="text/css" rel="stylesheet" media="screen" href="https://2014.igem.org/Team:HIT-Harbin/css/contentstyle.css?action=raw&amp;ctype=text/css">
 
-
<link type="text/css" rel="stylesheet" media="screen" href="https://2014.igem.org/Team:HIT-Harbin/css/judging.css?action=raw&amp;ctype=text/css">
 
-
<script type="text/javascript" src="https://2014.igem.org/Team:HIT-Harbin/js/main.js?action=raw&amp;ctype=text/javascript"></script>
 
-
<!--main content -->
 
-
<!--topCentent content -->
 
-
<div id="topCentent">
 
-
  <!--navigation -->
 
-
  <div id="nav">
 
-
    <!--project -->
 
-
    <div id="project" class="nav1" onmouseover="displaySubMenu(this)" onmouseout="hideSubMenu(this)">                                    <a href="https://2014.igem.org/Team:HIT-Harbin/Project" name="top">Project</a>
 
-
      <ul>
 
-
        <li>Abstract</li>
 
-
        <li>Background</li>
 
-
        <li>Design</li>
 
-
        <li>Modeling</li>
 
-
        <li>Results</li>
 
-
        <li>Future Work</li>
 
-
      </ul>
 
-
    </div>
 
-
    <!--Judging-->
 
-
    <div id="judging" class="nav1" onmouseover="displaySubMenu(this)" onmouseout="hideSubMenu(this)"><a href="https://2014.igem.org/Team:HIT-Harbin/Judging">Judging</a>
 
-
      <ul>
 
-
        <li>Biobrick</li>
 
-
        <li>Achievement</li>
 
-
    </div>
 
-
    <!--Wetlab-->
 
-
    <div id="wetlab" class="nav1" onmouseover="displaySubMenu(this)" onmouseout="hideSubMenu(this)"><a href="https://2014.igem.org/Team:HIT-Harbin/Wetlab">Wetlab</a>
 
-
      <ul>
 
-
        <li>Protocols</li>
 
-
        <li>Notebook</li>
 
-
    </div>
 
-
    <!--safety -->
 
-
    <div id="safety" class="nav1" onmouseover="displaySubMenu(this)" onmouseout="hideSubMenu(this)"><a href="https://2014.igem.org/Team:HIT-Harbin/Safety">Safety</a>
 
-
    </div>
 
-
    <!--Team -->
 
-
    <div id="team" class="nav1" onmouseover="displaySubMenu(this)" onmouseout="hideSubMenu(this)"><a href="https://2014.igem.org/Team:HIT-Harbin/Team">Team</a>
 
-
      <ul>
 
-
        <li>Members</li>
 
-
        <li>Advisors</li>
 
-
        <li>Instructors</li>
 
-
        <li>Attribution</li>
 
-
        <li>Acknowledge</li>
 
-
      </ul>
 
-
    </div>
 
-
    <!--humanPractic -->
 
-
    <div id="humanPractices" class="nav1" onmouseover="displaySubMenu(this)" onmouseout="hideSubMenu(this)"><a href="https://2014.igem.org/Team:HIT-Harbin/Humanpractices">Human&nbsp;&nbsp;practices</a>
 
-
      <ul>
 
-
        <li>Country Profile</li>
 
-
        <li>Qualification Trials</li>
 
-
        <li>Wechat Platform</li>
 
-
        <li>Video</li>
 
-
        <li>Presentation</li>
 
-
      </ul>
 
-
    </div>
 
-
  </div>
 
-
<!--mainarea -->
 
-
  <div id="mainarea">
 
-
                <div id="subheader-bar">
 
-
                    <div class="site-description"><h4><span class="colored"><cufon class="cufon cufon-canvas" alt="Parts" style="width: 54px; height: 20px;"><canvas width="60" height="27" style="width: 60px; height: 27px; top: -7px; left: 1px;"></canvas><cufontext>Parts</cufontext></cufon></span></h4></div>
 
-
                </div>
 
-
<p>Sequence optimization was done using JCat(Java Codon Adaptation Tool)[1] developed by  Andreas Grote </p>
 
-
<h5 class="hashed"><span>CODON OPTIMIZATION FOR HRPS AND HRPR GENE</span></h5>
 
-
<div align="center">
 
-
<img src="https://static.igem.org/mediawiki/2013/f/f8/Hrps_sequence_cai_before_optimization.png">
 
-
<p>Figure1: Codon Adaptation of hrpS gene in E.coli before optimization</p>
 
-
</div>
 
-
<p></p>CAI-Value of the sequence before optimization:0.2736.    GC-Content of the sequence before optimization:59.17%
 
-
<div align="center">
 
-
<img src="https://static.igem.org/mediawiki/2013/d/d3/Hrps_sequence_cai_after_optimization.png">
 
-
<p>Figure2: Codon Adaptation of hrpS gene in E.coli after optimization</p>
 
-
</div>
 
-
<p></p>CAI-Value of the optimized sequence:0.9832    GC-Content of the optimized sequence:53.60%
 
-
<div align="center">
 
-
<img src="https://static.igem.org/mediawiki/2013/a/a8/Hrpr_sequence_cai_before_optimization.png">
 
-
<p>Figure3: Codon Adaptation of hrpR gene in E.coli before optimization</p>
 
-
</div>
 
-
<p></p>CAI-Value of the sequence before optimization:0.2490.    GC-Content of the sequence before optimization:57.70
 
-
<div align="center">
 
-
<img src="https://static.igem.org/mediawiki/2013/8/8b/Hrpr_sequence_cai_after_optimization.png">
 
-
<p>Figure4: Codon Adaptation of hrpR gene in E.coli after optimization</p>
 
-
</div>
 
-
<p></p>CAI-Value of the optimized sequence:0.9721.    GC-Content of the optimized sequence:53.69%
 
-
<h4>reference</h4>
 
-
<p></p>[1] Andreas Grote et al.  JCat: a novel tool to adapt codon usage of a target gene to its potential expression host. Nucleic Acids Research 2005 Vol.33
 
-
</div>
 
-
</div>
 
-
<div id="bottomNav"><a href="#top">Back&nbsp;To&nbsp;Top</a></div>
 
<script>toolbar('nav')</script>
<script>toolbar('nav')</script>

Latest revision as of 18:46, 17 October 2014