Team:Bordeaux/NotebookJune3

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<div> <b>DAY TWO :</b> Inoculate 1L SOB with 12 mL overnight culture. Grow culture at 18°C (this temperature is really important as we see a 10-fold decrease in competency when we grow them at room temperature)</div>
<div> <b>DAY TWO :</b> Inoculate 1L SOB with 12 mL overnight culture. Grow culture at 18°C (this temperature is really important as we see a 10-fold decrease in competency when we grow them at room temperature)</div>
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<li> Preparation of LB medium (Agar for plates, +Ampicillin and +Chloramphenicol) </li>
<li> Preparation of LB medium (Agar for plates, +Ampicillin and +Chloramphenicol) </li>
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{{:Team:Bordeaux/Pied}}

Latest revision as of 18:40, 17 October 2014

3rd June

Molecular biology

  • DAY TWO : Inoculate 1L SOB with 12 mL overnight culture. Grow culture at 18°C (this temperature is really important as we see a 10-fold decrease in competency when we grow them at room temperature)

  • Preparation of LB medium (Agar for plates, +Ampicillin and +Chloramphenicol)