Team:TCU Taiwan/Notebook

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   <legend class="qnum" align="center"><font face="Trebuchet MS" size="6" color="#82C0AF">Bacteria strains and others</font><br>
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   <legend class="qnum" align="center"><font face="Trebuchet MS" size="6" color="#82C0AF">Bacteria strains and others</font><br></legend>
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   <p>*DH5a<br>
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   <font size="3" face="Verdana" color="#333"><p>*DH5a<br>
     *JM101<br>
     *JM101<br>
     *0.8%  and 2% agarose gel<br>
     *0.8%  and 2% agarose gel<br>
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     *1.5  ml centrifuge tube<br>
     *1.5  ml centrifuge tube<br>
     *250  ml flask<br>
     *250  ml flask<br>
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     *M13KO7(1*1011  pfu/ml)from <em>NEW ENGLAND BioLabs</em></p>
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     *M13KO7(1*1011  pfu/ml)from <em>NEW ENGLAND BioLabs</em></p></font>
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Latest revision as of 18:19, 17 October 2014

 
Notebook
  
iGEM materials
Bacteria medium

*LB(Luria-Bertani) broth: 1% Trytone, 1% NaCl, 0.5% Yeast extract in the ddH2O
*LB agar plate:LB broth add 1.5% agar.If you want to add selection marker example for ampicillin or chloramphenicol. To culture E.coli, ampicillin is added 100 mg/ml for usage, chloramphenicol is added 30 mg/ml for usage, kanamycin is added 70 mg/ml for usage
Plasmid isolation

*Solution I: 50 mM glucose、25 mM Tris-HCl (pH 8.0)、10 mM EDTA
*Solution II:0.2N NaOH and 1% SDS(Sodium dodecyl sulfate ) mix
*Solution III: 5 M potassium acetate (pH4.8), 11.5% glacial acetic acid
*RNase A(10 mg/ml)
*Phenol chloroform
*Chloroform
*95% Ethanol
*Tris-EDTA(TE)buffer:10 mM Tris-Cl(pH 7.5) 1 mM EDTA
*pBluescript SK(-)
*BBa_K1218011
*BBa_K914003
*BBa_I13521
Quick screening

*Quick screening buffer(Tris-Cl pH 12.6, 3% SDS)
*Phenol chloroform
*Chloroform
Enzyme digestion

*T4 ligase
*10X ligation buffer
*EcoRI
*PstI
*SpeI
*XbaI
*BSA 0.1%
Bacteria strains and others

*DH5a
*JM101
*0.8% and 2% agarose gel
*95% Ethanol
*6X loading dye
*1.5 ml centrifuge tube
*250 ml flask
*M13KO7(1*1011 pfu/ml)from NEW ENGLAND BioLabs
Polymerase chain reaction

*Top DNA polymerase
*dNTP(dATP, dCTP, dGTP, dTTP) 250 mM
*Tris-HCl(pH9.0) 10 mM
*KCl 30 mM
*MgCl2 1.5 mM
Instruments

*VCM-620, laminal flow
*Biofuge pico, Heraeus
* OS1500-H, TSK Oribital Shaking Iucubator
* Biowave
* Vortex-GENE 2
* Autoclave AS-1060L
* GenAmp PCR System 2400, Applied Biosystems
*BU-420, YIHDER
*5804R, eppendorf Centrifuge
Life Records
   
 
 
 
Study Together
 
 
 
Conference in NCTU
 
 
Trip in Chihsingtan Beach
 
 
 
 
^


    
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