Team:ULB-Brussels

From 2014.igem.org

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<tr style="background-color:rgb(245,245,245); box-shadow: 1px 1px 12px #555;"><p class="title"> Welcome to our website</p></tr>
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<section style="text-align: justify"></br></br></br><p>This year, the <i>Université Libre de Bruxelles</i> (ULB) competes again in the International Genetically Engineered Machines contest, which is a competition of synthetic biology organized by the Massachussetts Institute of Technology. This edition of 2014 will mark the 10th anniversary of the IGEM and will be characterized by a "Giant Jamboree", where all teams around the world will be invited in Boston without regional pre-selection !</p>
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<p>Our team will present a new system which aims to fight against the emergence of sub-populations of cells that are less or no productive in bioreactors by coupling recombinant protein production with a essential protein, thus addicting cells to production. We hope this system will increase the yields of production by some percents. For more details, please see the page dedicated to the project !</p>
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<p>On this website, you can also find all the informations about our sponsors and the evolution of the project. Feel free to browse!</p></section>
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<section style="text-align: justify; margin: 25px"><p>$\Tgo\hspace{-0.145cm}$his year, the Université Libre de Bruxelles competes again in the International Genetically Engineered Machines contest, a competition of synthetic biology organized by the Massachussetts Institute of Technology. Year 2014 will mark the 10th anniversary of the IGEM and will feature a "Giant Jamboree", where all teams will be invited in Boston without regional pre-selection.<br><br>
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Our aim is to enhance recombinant protein production in bioreactors by addicting microorganisms to the production of a protein of interest (PI).  
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We built $\MyColi$, a recombinant <i>Escherichia Coli</i> expressing a toxin and the PI. This protein will be on the same open reading frame as an antoxin, a protein able to neutralize the effects of the toxin. Stressed subpopulations might not express the PI (and thus the antitoxin) to a sufficient level, allowing the toxin to kill unproductive cells. Our work will consist on building and modelling this system throughout the summer.  </p></section>
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Latest revision as of 18:11, 17 October 2014

$~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ \newcommand{\MyColi}{{\small Mighty\hspace{0.12cm}Coli}} \newcommand{\Stabi}{\small Stabi}$ $\newcommand{\EColi}{\small E.coli} \newcommand{\SCere}{\small S.cerevisae}\\[0cm] ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ \newcommand{\PI}{\small PI}$ $\newcommand{\Igo}{\Large\mathcal{I}} \newcommand{\Tgo}{\Large\mathcal{T}} \newcommand{\Ogo}{\Large\mathcal{O}} ~$ Example of a hierarchical menu in CSS

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- Université Libre de Bruxelles -


$Welcome$ 'to our Website'


$\Tgo\hspace{-0.145cm}$his year, the Université Libre de Bruxelles competes again in the International Genetically Engineered Machines contest, a competition of synthetic biology organized by the Massachussetts Institute of Technology. Year 2014 will mark the 10th anniversary of the IGEM and will feature a "Giant Jamboree", where all teams will be invited in Boston without regional pre-selection.

Our aim is to enhance recombinant protein production in bioreactors by addicting microorganisms to the production of a protein of interest (PI). We built $\MyColi$, a recombinant Escherichia Coli expressing a toxin and the PI. This protein will be on the same open reading frame as an antoxin, a protein able to neutralize the effects of the toxin. Stressed subpopulations might not express the PI (and thus the antitoxin) to a sufficient level, allowing the toxin to kill unproductive cells. Our work will consist on building and modelling this system throughout the summer.



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