Team:ULB-Brussels

From 2014.igem.org

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<section style="text-align: justify; margin: 25px"><p>$\Tgo\hspace{-0.145cm}$his year, the Université Libre de Bruxelles competes again in the International Genetically Engineered Machines contest, a competition of synthetic biology organized by the Massachussetts Institute of Technology. Year 2014 will mark the 10th anniversary of the IGEM and will feature a "Giant Jamboree", where all teams will be invited in Boston without regional pre-selection.<br><br>
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Our aim is to enhance recombinant protein production in bioreactors by addicting microorganisms to the production of a protein of interest (PI).  
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<tr style="background-color:rgb(245,245,245);"><td colspan="3"> <h3> Requirements </h3></td></tr>
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We built $\MyColi$, a recombinant <i>Escherichia Coli</i> expressing a toxin and the PI. This protein will be on the same open reading frame as an antoxin, a protein able to neutralize the effects of the toxin. Stressed subpopulations might not express the PI (and thus the antitoxin) to a sufficient level, allowing the toxin to kill unproductive cells. Our work will consist on building and modelling this system throughout the summer. </p></section>
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<p> Please be sure to keep these links, your audience will want to find your: </p>
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<li><a href="https://2014.igem.org/Team:ULB-Brussels">Home</a> </li>
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<li><a href="https://2014.igem.org/Team:ULB-Brussels/Team">Team</a> </li>
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<li><a href="https://igem.org/Team.cgi?year=2013&team_name=ULB-Brussels">Official Team Profile</a> </li>
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<li><a href="https://2014.igem.org/Team:ULB-Brussels/Project">Project</a> </li>
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<li><a href="https://2014.igem.org/Team:ULB-Brussels/Parts">Parts</a> </li>
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<li><a href="https://2014.igem.org/Team:ULB-Brussels/Modeling">Modeling</a> </li>
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<li><a href="https://2014.igem.org/Team:ULB-Brussels/Notebook">Notebook</a> </li>
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<li><a href="https://2014.igem.org/Team:ULB-Brussels/Safety">Safety</a> </li>
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<li><a href="https://2014.igem.org/Team:ULB-Brussels/Attributions">Attributions</a> </li>
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<p>There are a few wiki requirements teams must follow:</p>
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<li>All pages, images and files must be hosted on the <a href ="https://2014.igem.org/Special:Upload">  2014.igem.org server</a>. </li>
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<li>All pages must be created under the team’s name space.</li>
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<li>As part of your documentation, keep the links from the menu to the left. </li>
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<li>The <a href="https://static.igem.org/mediawiki/igem.org/6/60/Igemlogo_300px.png"> iGEM logo </a> should be placed on the upper part of every page and should link to <a href="https://2014.igem.org/Main_Page">2014.igem.org</a>.</li>
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<p>Visit the <a href="https://2014.igem.org/Wiki_How-To"> Wiki How To page </a> for a complete list of requirements, tips and other useful information. </p>
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<tr style="background-color:rgb(245,245,245);"><td colspan="3" > <h3> Tips  </h3></td></tr>
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<p>We are currently working on providing teams with some easy to use design templates.
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<br> In the meantime you can also view other team wikis for inspiration! Here are some very good examples</p>
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<li> <a href="https://2013.igem.org/Team:SDU-Denmark/"> 2013 SDU Denmark </a> </li>
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<li> <a href="https://2013.igem.org/Team:SYSU-China">2013 SYSU China</a> </li>
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<li> <a href="https://2013.igem.org/Team:Shenzhen_BGIC_ATCG"> 2013 Shenxhen BGIG ATCG </a></li>
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<li> <a href="https://2013.igem.org/Team:Colombia_Uniandes">2013 Colombia Unianades </a></li>
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<li> <a href="https://2013.igem.org/Team:Lethbridge">2013 Lethbridge</a></li>
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<p>For a full wiki list, you can visit <a href="https://igem.org/Team_Wikis?year=2013">iGEM 2013 web sites </a> and <a href="https://igem.org/Team_Wikis?year=2012">iGEM 2012 web sites</a>  lists. </p>
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<p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
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<li>State your accomplishments! Tell people what you have achieved from the start. </li>
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<li>Be clear about what you are doing and what you plan to do.</li>
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<li>You have a global audience! Consider the different backgrounds that your users come from.</li>
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<li>Make sure information is easy to find; nothing should be more than 3 clicks away.  </li>
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<li>Avoid using very small fonts and low contrast colors; information should be easy to read.  </li>
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<li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="">iGEM 2013 calendar</a> </li>
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<li>Have lots of fun! </li>
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<tr style="background-color:rgb(245,245,245);"><td colspan="3"> <p><br/></p><img width="75px" src="http://www.ulb.ac.be/dre/com/docs/sceau-a-quadri.jpg" alt="">&nbsp;&nbsp;Université Libre de Bruxelles - Scientia Vincere Tenebras </td></tr>
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Latest revision as of 18:11, 17 October 2014

$~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ \newcommand{\MyColi}{{\small Mighty\hspace{0.12cm}Coli}} \newcommand{\Stabi}{\small Stabi}$ $\newcommand{\EColi}{\small E.coli} \newcommand{\SCere}{\small S.cerevisae}\\[0cm] ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ \newcommand{\PI}{\small PI}$ $\newcommand{\Igo}{\Large\mathcal{I}} \newcommand{\Tgo}{\Large\mathcal{T}} \newcommand{\Ogo}{\Large\mathcal{O}} ~$ Example of a hierarchical menu in CSS

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- Université Libre de Bruxelles -


$Welcome$ 'to our Website'


$\Tgo\hspace{-0.145cm}$his year, the Université Libre de Bruxelles competes again in the International Genetically Engineered Machines contest, a competition of synthetic biology organized by the Massachussetts Institute of Technology. Year 2014 will mark the 10th anniversary of the IGEM and will feature a "Giant Jamboree", where all teams will be invited in Boston without regional pre-selection.

Our aim is to enhance recombinant protein production in bioreactors by addicting microorganisms to the production of a protein of interest (PI). We built $\MyColi$, a recombinant Escherichia Coli expressing a toxin and the PI. This protein will be on the same open reading frame as an antoxin, a protein able to neutralize the effects of the toxin. Stressed subpopulations might not express the PI (and thus the antitoxin) to a sufficient level, allowing the toxin to kill unproductive cells. Our work will consist on building and modelling this system throughout the summer.



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