Team:CSU Fort Collins/Notebook/Protocols=Gel

From 2014.igem.org

(Difference between revisions)
 
(16 intermediate revisions not shown)
Line 44: Line 44:
body {
body {
   line-height: 1;
   line-height: 1;
-
}
 
-
ol, ul {
 
-
  list-style: none;
 
}
}
blockquote, q {
blockquote, q {
Line 170: Line 167:
}
}
#cssmenu > ul > li:hover::after {
#cssmenu > ul > li:hover::after {
-
   border-top-color: #f2a76d;
+
   border-top-color: #E6B800;
}
}
#indicatorContainer {
#indicatorContainer {
Line 481: Line 478:
}
}
h2,h3,.toc,.show,.hide,.content{
h2,h3,.toc,.show,.hide,.content{
-
   color:#E6B800;
+
   color:#222222;
   font-family:'Bitter',serif;
   font-family:'Bitter',serif;
}
}
Line 493: Line 490:
   width:100%;
   width:100%;
   margin:auto;
   margin:auto;
 +
  border:none;
 +
  background-color:#efefef;
}
}
.toc .month-group { margin: 20px 0; }
.toc .month-group { margin: 20px 0; }
Line 586: Line 585:
.footer a{
.footer a{
     color:#efefef;
     color:#efefef;
 +
}
 +
#banner a:hover{
 +
opacity:0.8;
}
}
Line 597: Line 599:
<body style='margin:0'>
<body style='margin:0'>
-
<div id='banner'><center><img src='http://fbcdn-sphotos-e-a.akamaihd.net/hphotos-ak-xpa1/v/t1.0-9/10703497_10203586438474233_7822191246808094941_n.jpg?oh=f0decc1a5a8158cdcb804158ab37ab5c&oe=54CA26F0&__gda__=1421672678_3032b2e395a8cebe2ec61921b5725067' style='width:100%; margin-top:-400px'/>
+
<div id='banner'><center><img src='http://oi61.tinypic.com/vzhceb.jpg' style='width:100%; margin-top:-400px'/><a href='https://2014.igem.org/Main_Page'><img src='https://static.igem.org/mediawiki/2014/d/d7/Sysuchina_igemdeLogo.png' style="width:80px; position:absolute; top: 10px; right: 20px; opacity:0.95;"/></a>
</center></div>
</center></div>
Line 614: Line 616:
       <ul>
       <ul>
         <li><a href='/Team:CSU_Fort_Collins/Members/'><span>Members</span></a></li>
         <li><a href='/Team:CSU_Fort_Collins/Members/'><span>Members</span></a></li>
-
         <li class='last'><a href='/Team:CSU_Fort_Collins/Sponsors/'><span>Sponsors</span></a></li>
+
        <li><a href='/Team:CSU_Fort_Collins/Sponsors/'><span>Sponsors</span></a></li>
 +
         <li class='last'><a href='/Team:CSU_Fort_Collins/Acknowledgements/'><span>Acknowledgements</span></a></li>
       </ul>
       </ul>
   </li>
   </li>
Line 631: Line 634:
       <li class='has-sub'><a href='/Team:CSU_Fort_Collins/Notebook/DailyNotes'><span>Daily Notes</span></a>
       <li class='has-sub'><a href='/Team:CSU_Fort_Collins/Notebook/DailyNotes'><span>Daily Notes</span></a>
         <ul>
         <ul>
-
         <li class='has-sub'><a href='/Team:CSU_Fort_Collins/Notebook/Biosensor/'><span>Biosensor</span></a>
+
         <li class='has-sub'><a href='/Team:CSU_Fort_Collins/Notebook/Biosensor/Jun'><span>Biosensor</span></a>
             <ul>
             <ul>
-
               <li><a href="#"><span>June</span></a></li>
+
               <li><a href="/Team:CSU_Fort_Collins/Notebook/Biosensor/Jun"><span>June</span></a></li>
-
               <li><a href="#"><span>July</span></a></li>
+
               <li><a href="/Team:CSU_Fort_Collins/Notebook/Biosensor/Jul"><span>July</span></a></li>
-
              <li><a href='#'><span>August</span></a></li>
+
               <li class='last'><a href='/Team:CSU_Fort_Collins/Notebook/Biosensor/Aug'><span>August</span></a></li>
-
               <li class='last'><a href="#"><span>September</span></a></li>
+
             </ul>
             </ul>
         </li>
         </li>
-
         <li class='has-sub'><a href='/Team:CSU_Fort_Collins/Notebook/Breakdown/'><span>Breakdown</span></a>
+
         <li class='has-sub'><a href='/Team:CSU_Fort_Collins/Notebook/Breakdown/Jul'><span>Breakdown</span></a>
             <ul>
             <ul>
-
               <li><a href="#"><span>July</span></a></li>
+
               <li><a href="/Team:CSU_Fort_Collins/Notebook/Breakdown/Jul"><span>July</span></a></li>
-
               <li><a href='#'><span>August</span></a></li>
+
               <li><a href='/Team:CSU_Fort_Collins/Notebook/Breakdown/Aug'><span>August</span></a></li>
-
               <li class='last'><a href="#"><span>September</span></a></li>
+
               <li class='last'><a href="/Team:CSU_Fort_Collins/Notebook/Breakdown/Sep"><span>September</span></a></li>
             </ul>
             </ul>
         </li>
         </li>
-
         <li class='has-sub'><a href='/Team:CSU_Fort_Collins/Notebook/HVP/'><span>High-Value Product</span></a>
+
         <li class='has-sub'><a href='/Team:CSU_Fort_Collins/Notebook/HVP/Jun'><span>High-Value Product</span></a>
             <ul>
             <ul>
-
               <li><a href="#"><span>June</span></a></li>
+
               <li><a href="/Team:CSU_Fort_Collins/Notebook/HVP/Jun"><span>June</span></a></li>
-
              <li><a href="#"><span>July</span></a></li>
+
               <li class='last'><a href="/Team:CSU_Fort_Collins/Notebook/HVP/Jul"><span>July</span></a></li>
-
              <li><a href='#'><span>August</span></a></li>
+
-
               <li class='last'><a href="#"><span>September</span></a></li>
+
             </ul>
             </ul>
         </li>
         </li>
-
         <li li class='has-sub'><a href='/Team:CSU_Fort_Collins/Notebook/KillSwitch/'><span>Kill Switch</span></a>
+
         <li li class='has-sub'><a href='/Team:CSU_Fort_Collins/Notebook/KillSwitch/Jul'><span>Kill Switch</span></a>
             <ul>
             <ul>
-
               <li><a href="#"><span>July</span></a></li>
+
               <li><a href="/Team:CSU_Fort_Collins/Notebook/KillSwitch/Jul"><span>July</span></a></li>
-
              <li><a href='#'><span>August</span></a></li>
+
               <li class='last'><a href="/Team:CSU_Fort_Collins/Notebook/KillSwitch/Sep"><span>September</span></a></li>
-
               <li class='last'><a href="#"><span>September</span></a></li>
+
             </ul>
             </ul>
         </li>
         </li>
Line 665: Line 664:
   </ul>
   </ul>
   </li>
   </li>
-
   <li class='has-sub'><a href='/Team:CSU_Fort_Collins/HumPrac/'><span>Human Practices</span></a>
+
   <li class='has-sub'><a href='/Team:CSU_Fort_Collins/Collab/'><span>Human Practices</span></a>
       <ul>
       <ul>
         <li><a href='/Team:CSU_Fort_Collins/Collab/'><span>Collaboration</span></a></li>
         <li><a href='/Team:CSU_Fort_Collins/Collab/'><span>Collaboration</span></a></li>
Line 671: Line 670:
       </ul>
       </ul>
   </li>
   </li>
-
   <li class='has-sub'><a href='/Team:CSU_Fort_Collins/Achievements/'><span>Achievements</span></a>
+
   <li class='has-sub'><a href='/Team:CSU_Fort_Collins/Parts/'><span>Achievements</span></a>
       <ul>
       <ul>
         <li><a href='/Team:CSU_Fort_Collins/Parts/'><span>Parts</span></a></li>
         <li><a href='/Team:CSU_Fort_Collins/Parts/'><span>Parts</span></a></li>
Line 677: Line 676:
       </ul>
       </ul>
   </li>
   </li>
-
   <li><a href='/Team:CSU_Fort_Collins/Safety/'><span>Safety</span></a></li>
+
   <li class='last'><a href='/Team:CSU_Fort_Collins/Safety/'><span>Safety</span></a></li>
</ul>
</ul>
</div>
</div>
-
 
Line 686: Line 684:
<div class='wrapper'>
<div class='wrapper'>
-
   <center>
+
   <center><h1>Gel Electrophoresis Protocol</h1>
-
    <h1>Welcome to CSU Fort Collin's 2014 Wiki</h1>
+
-
 
+
-
    <div class="container">
+
-
      <h3>Gel Electrophoresis Protocol</h3>
+
-
        
+
<div class='show'>Show Table of Contents</div>
-
    </div>
+
  <div class='toc'>
-
  </center>
+
    <div class='hide'>Hide Table of Contents</div><br><br>
-
</div>
+
       <a href='#' id='ftoc'>&#8592; Full Table of Contents</a><br>
 +
      <div class="month-group">
 +
        <a href='#'>Gibson Assembly</a>
 +
      </div>
 +
      <div class="month-group">
 +
        <a href='#'>Cloning Genes</a>
 +
      </div>
 +
      <div class="month-group">
 +
        <a href='#'>Plasmid Miniprep</a>
 +
      </div>
 +
      <div class="month-group">
 +
        <a href='#'>Yeast DNA Extraction</a>
 +
      </div>
 +
      <div class="month-group">
 +
        <a href='#'>Gel Electrophoresis</a>
 +
      </div>
 +
      <div class="month-group">
 +
        <a href='#'>PCR Product Purification</a>
 +
      </div>
 +
  </div>
-
<div class="footer">
+
  <div class='page'>
-
     <center><table><center><td><p>© Colorado State University iGEM 2014 | Colorado State University, Campus Delivery 1370, Fort Collins, CO 80523-1370 | <a href="/Team:CSU_Fort_Collins/Contact/" > Contact Us </a>| <a href="/Team:CSU_Fort_Collins/Sponsors/"> Become a Sponsor </a> | <a href="http://www.facebook.com/csu.igem.2014" >Facebook</a> | <a href="http://twitter.com/CSU_iGEM" >Twitter</a></td></center></p>
+
     <p>
-
 
+
-
</table></center>
+
-
</div>
+
 +
      <ol>
 +
          <li>Using a balance, mass out 1.0 gram of agarose. Mix this with 100 mL of TAE 1X Buffer.</li>
 +
          <li>Microwave the mixture, mixing intermittently, until all the agarose is dissolved and the mixture is homogeneous.</li>
 +
          <li>Pour the mixture into a gel plate and insert an 8-lane comb. Allow to cool and solidify in the fridge or on the benchtop</li>
 +
          <li>Mix your samples to run in the gel. Use 10 &#956;L of the DNA Ladder combined with 2 &#956;L SYBR Green in the first lane and for all samples, mix 5 &#956;L of sample with 5 &#956;L of nuclease-free water and 2 &#956;L of SYBR Green/Dye Mixture.</li>
 +
          <li>Place gel in tray into the gel electrophoresis apparatus; Wells should be located towards the negative (conventionally black) end. Fill apparatus with 1X TAE until the gel is completely submerged. Do not overfill.</li>
 +
          <li>Load 10 &#956;L of each sample into the appropriate wells.</li>
 +
          <li>Connect wiring and run gel electrophoresis for 1 hour.</li>
 +
          <li>Analyze gel in UV light and see if samples match the expected size using the ladder as a guide.</li>
 +
    </ol>
 +
 +
    </p>
 +
    <br><br>
 +
 +
    <center><a href='/Team:CSU_Fort_Collins/Notebook/Protocols=Isolation' id='navi' style='margin-left:-20px; margin-right:40px'> Previous</a> <a href='/Team:CSU_Fort_Collins/Notebook/Protocols=Purify' id='navi'>Next </a></center>
 +
  </div>
 +
 +
<br><br>
 +
</div>

Latest revision as of 17:50, 17 October 2014

CSU iGEM 2014

Gel Electrophoresis Protocol

Show Table of Contents
Hide Table of Contents


← Full Table of Contents
Gibson Assembly
Cloning Genes
Plasmid Miniprep
Yeast DNA Extraction
Gel Electrophoresis
PCR Product Purification

  1. Using a balance, mass out 1.0 gram of agarose. Mix this with 100 mL of TAE 1X Buffer.
  2. Microwave the mixture, mixing intermittently, until all the agarose is dissolved and the mixture is homogeneous.
  3. Pour the mixture into a gel plate and insert an 8-lane comb. Allow to cool and solidify in the fridge or on the benchtop
  4. Mix your samples to run in the gel. Use 10 μL of the DNA Ladder combined with 2 μL SYBR Green in the first lane and for all samples, mix 5 μL of sample with 5 μL of nuclease-free water and 2 μL of SYBR Green/Dye Mixture.
  5. Place gel in tray into the gel electrophoresis apparatus; Wells should be located towards the negative (conventionally black) end. Fill apparatus with 1X TAE until the gel is completely submerged. Do not overfill.
  6. Load 10 μL of each sample into the appropriate wells.
  7. Connect wiring and run gel electrophoresis for 1 hour.
  8. Analyze gel in UV light and see if samples match the expected size using the ladder as a guide.



Previous Next


Retrieved from "http://2014.igem.org/Team:CSU_Fort_Collins/Notebook/Protocols%3DGel"