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Team:Groningen/Template/MODULE/Notebook/characterisation/week3
From 2014.igem.org
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| - | Fusion of the NisA BioBrick (BBa_K1365000) and the Superfolded GFP BioBrick (BBa_K1365020) with a double terminator (BBa_B0015) | + | Fusion of the NisA BioBrick (<a href="http://parts.igem.org/Part:BBa_K1365000">BBa_K1365000</a>) and the Superfolded GFP BioBrick (<a href="http://parts.igem.org/Part:BBa_K1365020">BBa_K1365020</a>) with a double terminator (<a href="http://parts.igem.org/Part:BBa_B0015">BBa_B0015</a>) |
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<div class="item">The size of the plasmids were confirmed by single and double digestion of the plasmids (pSB1C3) with the NisA and Superfolded GFP BioBricks to confirm the sizes of the plasmids</div> | <div class="item">The size of the plasmids were confirmed by single and double digestion of the plasmids (pSB1C3) with the NisA and Superfolded GFP BioBricks to confirm the sizes of the plasmids</div> | ||
| - | <div class="item">Both, the NisA and the Superfolded GFP | + | <div class="item">Both, the NisA and the Superfolded GFP BioBricks, were ligated into pSB1A3 together with the double terminator as a second insert</div> |
<div class="item">The ligation was performed overnight, after which the ligated plasmids were transformed to two different <i>E. coli</i> DH5α strains</div> | <div class="item">The ligation was performed overnight, after which the ligated plasmids were transformed to two different <i>E. coli</i> DH5α strains</div> | ||
<div class="item">The size of the construct was determined by colony PCR</div> | <div class="item">The size of the construct was determined by colony PCR</div> | ||
Latest revision as of 17:46, 17 October 2014
September 15 - September 21
Fusion of the NisA BioBrick (BBa_K1365000) and the Superfolded GFP BioBrick (BBa_K1365020) with a double terminator (BBa_B0015)
The size of the plasmids were confirmed by single and double digestion of the plasmids (pSB1C3) with the NisA and Superfolded GFP BioBricks to confirm the sizes of the plasmids
Both, the NisA and the Superfolded GFP BioBricks, were ligated into pSB1A3 together with the double terminator as a second insert
The ligation was performed overnight, after which the ligated plasmids were transformed to two different E. coli DH5α strains
The size of the construct was determined by colony PCR
A double digestion was performed, and the constructs were further purified
