Team:Paris Saclay/Notebook/July/11

From 2014.igem.org

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(→Friday 11th July)
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[https://2014.igem.org/Team:Paris_Saclay/Protocols/Transformation_of_supercompetent_Ecoli_cells_with_CaCl2 Protocol]
[https://2014.igem.org/Team:Paris_Saclay/Protocols/Transformation_of_supercompetent_Ecoli_cells_with_CaCl2 Protocol]
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====3 - Electrophoresis====
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''Fabio & Marie''
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#Cm FNR-RBS-AmilCP term (1)
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#Cm FNR-RBS-AmilCP term (2)
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#B0015 (1)
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#B0015 (2)
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#PCola-GES 10^-2 (1)
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#PCola-GES 10^-2 (1)
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#PCola-GES 10^-2 (2)
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#PCola-GES 10^-2 (2)
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#PJBG-6409 10^-2 (1)
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#PJBG-6409 10^-2 (2)
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#PJBG-6409 10^-2 (2)
[https://2014.igem.org/Team:Paris_Saclay/Notebook Back to the calendar]
[https://2014.igem.org/Team:Paris_Saclay/Notebook Back to the calendar]

Revision as of 08:01, 15 July 2014

Contents

Friday 11th July

Lab work

1 - Fill in the WIKI (Notebook and Protocols)

Juliette & Romain

2 - Preparation of competent cells E.coli

Maher and Sean

Competent strains used:

  • DH5α
  • MG1655Z1

Sean did the day1, Maher did the day 2.

Protocol

3 - Electrophoresis

Fabio & Marie

  1. Cm FNR-RBS-AmilCP term (1)
  2. Cm FNR-RBS-AmilCP term (2)
  3. B0015 (1)
  4. B0015 (2)
  5. PCola-GES 10^-2 (1)
  6. PCola-GES 10^-2 (1)
  7. PCola-GES 10^-2 (2)
  8. PCola-GES 10^-2 (2)
  9. PJBG-6409 10^-2 (1)
  10. PJBG-6409 10^-2 (2)
  11. PJBG-6409 10^-2 (2)

Back to the calendar

Retrieved from "http://2014.igem.org/Team:Paris_Saclay/Notebook/July/11"