Team:Paris Saclay/Notebook/July/11

From 2014.igem.org

(Difference between revisions)
(Friday 11th July)
(Friday 11th July)
Line 7: Line 7:
''Juliette & Romain''
''Juliette & Romain''
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====2 - Transformation of competent cells E.coli====
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====2 - Preparation of competent cells E.coli====
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''Fabio & Maher''
+
''Maher and Sean''
Competent strains used:  
Competent strains used:  
*DH5α
*DH5α
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*MG1655
 
*MG1655Z1
*MG1655Z1
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*DH5α+ (given by Solenne)
 
-
(The strains DH5α, MG1655 and MG1655Z1 were prepared by ''Mathias & Mathieu'')
 
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BioBricks used:
+
Sean did the day1, Maher did the day 2.
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*J23100 (Amp R)(Dilutions: Concentrated, 0 and 10^-1)
+
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*J23106 (Amp R)(Dilutions: Concentrated, 0 and 10^-1)
+
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*J23114 (Amp R)(Dilutions: Concentrated, 0 and 10^-1)
+
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+ pET28a (Kan R)(Dilutions: 0, 10^-1 and 10^-2)
+
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(pET28a is a plasmid used to verify that the 4 strains are competent).
+
-
 
+
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The negative controles:
+
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*pET28a: without plasmid, nothing grows.
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*J23100, J23106, J23114: without the BioBrick, nothing brows.
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-
 
+
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Results:
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*pET28a: Nothing grows with the strains DH5α, MG1655, MG1655Z1. But the strain DH5α+ grows well.
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*J23100, J23106, J23114: The strain DH5α+ grow well and the 3 others strains grow less. They have to be more concentrated.
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[https://2014.igem.org/Team:Paris_Saclay/Protocols/Transformation_of_supercompetent_Ecoli_cells_with_CaCl2 Protocol]
[https://2014.igem.org/Team:Paris_Saclay/Protocols/Transformation_of_supercompetent_Ecoli_cells_with_CaCl2 Protocol]
[https://2014.igem.org/Team:Paris_Saclay/Notebook Back to the calendar]
[https://2014.igem.org/Team:Paris_Saclay/Notebook Back to the calendar]

Revision as of 07:46, 15 July 2014

Contents

Friday 11th July

Lab work

1 - Fill in the WIKI (Notebook and Protocols)

Juliette & Romain

2 - Preparation of competent cells E.coli

Maher and Sean

Competent strains used:

  • DH5α
  • MG1655Z1

Sean did the day1, Maher did the day 2.

Protocol

Back to the calendar