"
Team:INSA-Lyon/Parts
From 2014.igem.org
| Line 16: | Line 16: | ||
<a href="https://2014.igem.org/Team:INSA-Lyon/Notebook" class="hu-icon"><li class="icon">NOTEBOOK</li></a> | <a href="https://2014.igem.org/Team:INSA-Lyon/Notebook" class="hu-icon"><li class="icon">NOTEBOOK</li></a> | ||
<a href="https://2014.igem.org/Team:INSA-Lyon/Protocol" class="hu-icon"><li class="icon">PROTOCOLS</li></a> | <a href="https://2014.igem.org/Team:INSA-Lyon/Protocol" class="hu-icon"><li class="icon">PROTOCOLS</li></a> | ||
| - | |||
<a href="https://2014.igem.org/Team:INSA-Lyon/DataPage" class="hu-icon"><li class="icon">DATA PAGE</li></a> | <a href="https://2014.igem.org/Team:INSA-Lyon/DataPage" class="hu-icon"><li class="icon">DATA PAGE</li></a> | ||
</ul> | </ul> | ||
Latest revision as of 17:07, 17 October 2014
-
-
PROJECT
PROJECT- PROJECT SUMMARY
- ACHIEVEMENTS
-
WETLAB
-
MODELING
-
HUMAN PRACTICE
HUMAN
PRACTICE- HUMAN PRACTICE SUMMARY
- SYNBIO PERCEPTION
- PROJECT'S INTEREST
-
TEAM
5 biobricks have been constructed, thre for Nickel chelation, two for promoter characterization.
Biobricks for nickel chelationThe principal of the construction is shown in the figure below.
On a pSB1C3 backbone, two essential part have been assembled: a promoter and a reporter coding for CsgA.The promoter in this case is always the same: P70.However, the reporter is different for each construction. The first CsgA is called CsgA WT, the sequence is exactly the same as we found in a wild type CsgA protein except one mutation in order to eliminate one restriction site. The construction is also called CsgA-WT. The second CsgA is based on the same sequence but with a short sequence coding for a his tag(a peptide chain coding six histidines). This construction is called CsgA-His1. The last CsgA is almost the same but with 2 his tag. This construction is called CsgA-His2.
