Team:INSA-Lyon/Parts

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<a href="https://2014.igem.org/Team:INSA-Lyon/Biology" class="hu-icon"><li class="iconmulti"  style="line-height:20px;  ">WETLAB SUMMARY</li></a>
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<a href="https://2014.igem.org/Team:INSA-Lyon/Biology" class="hu-icon"><li class="iconmulti">WETLAB SUMMARY</li></a>
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<a href="https://2014.igem.org/Team:INSA-Lyon/Results" class="hu-icon"><li class="iconmulti"  style="line-height:40px;  ">RESULTS</li></a>
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<a href="https://2014.igem.org/Team:INSA-Lyon/Results" class="hu-icon"><li class="icon">RESULTS</li></a>
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<a href="https://2014.igem.org/Team:INSA-Lyon/Notebook" class="hu-icon"><li class="iconmulti"  style="line-height:40px;  ">NOTEBOOK</li></a>
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<a href="https://2014.igem.org/Team:INSA-Lyon/Notebook" class="hu-icon"><li class="icon">NOTEBOOK</li></a>
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<a href="https://2014.igem.org/Team:INSA-Lyon/Parts" class="hu-icon"><li class="iconmulti"  style="line-height:40px;  ">PARTS</li></a>
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<a href="https://2014.igem.org/Team:INSA-Lyon/Protocol" class="hu-icon"><li class="icon">PROTOCOLS</li></a>
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<a href="https://2014.igem.org/Team:INSA-Lyon/DataPage" class="hu-icon"><li class="iconmulti"  style="line-height:40px;  ">DATA PAGE</li></a>
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<a href="https://2014.igem.org/Team:INSA-Lyon/DataPage" class="hu-icon"><li class="icon">DATA PAGE</li></a>
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<p>5 biobricks have been constructed, three for Nickel chelation, three for promoter characterization.</p></br></br></br><div>
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<p>5 biobricks have been constructed, thre for Nickel chelation, two for promoter characterization.</p></br>
<b>Biobricks for nickel chelation</b></br></br>
<b>Biobricks for nickel chelation</b></br></br>
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<p>The principal of the construction is shown in the figure below.</p></br></br></br>
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<p>The principal of the construction is shown in the figure below.</p></br></br></br></div>
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<img src= "https://static.igem.org/mediawiki/parts/6/63/1.JPG"></br>
<img src= "https://static.igem.org/mediawiki/parts/6/63/1.JPG"></br>
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<p>On a pSB1C3 backbone, two essential part have been assembled: a promoter and a reporter coding for CsgA.The promoter in this case is always the same: P70.However, the reporter is different for each construction.<div>
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<p>On a pSB1C3 backbone, two essential part have been assembled: a promoter and a reporter coding for CsgA.The promoter in this case is always the same: P70.However, the reporter is different for each construction.</br>
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The first CsgA is called CsgA WT, the sequence is exactly the same as we found in a wild type CsgA protein except one mutation in order to eliminate one restriction site. The construction is also called CsgA-WT.</br>
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The second CsgA is based on the same sequence but with a short sequence coding for a his tag(a peptide chain coding six histidines). This construction is called CsgA-His1.</br>
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The last CsgA is almost the same but with 2 his tag. This construction is called CsgA-His2.</br>
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<p>The first CsgA is called CsgA WT, the sequence is exactly the same as we found in a wild type CsgA protein except one mutation in order to eliminate one restriction site. The construction is also called CsgA.</p></br></br></br><div>
 
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<p>The second CsgA is based on the same sequence but with a short sequence coding for a his tag(a peptide chain coding six histidines). This construction is called His1.</p></br></br></br><div>
 
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<p>The last CsgA is almost the same but with 2 his tag. This construction is called His2.</p></br></br></br><div>
 
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<p>5 biobricks have been constructed, three for Nickel chelation, three for promoter characterization.</p></br></br></br><div>
 
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<b>Biobrick for promoter characterization</b></br></br>
 
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<p>The principal of the construction is shown in the figure below.</p></br></br></br>
 
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<img src= "https://static.igem.org/mediawiki/parts/f/fb/2.png"></br>
 
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<p>On a pKK backbone, two essential parts have been assembled: a promoter and a reporter.
 
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The reporter in this case is always the same: gfp. However, the promoter is different for each construction.<div>
 
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<p>The first promoter is P70, sequence found in the Resgistry. This construction is called p70::gfp</p></br></br></br><div>
 
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<p>The second promoter is PCurli, obtained from a PCR, sequence coding for the inter-genic regulation for curli production. This construction is called pcurli::gfp.</p></br></br></br><div>
 
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Latest revision as of 17:07, 17 October 2014

Curly'on - IGEM 2014 INSA-LYON

  • 5 biobricks have been constructed, thre for Nickel chelation, two for promoter characterization.


    Biobricks for nickel chelation

    The principal of the construction is shown in the figure below.





  • On a pSB1C3 backbone, two essential part have been assembled: a promoter and a reporter coding for CsgA.The promoter in this case is always the same: P70.However, the reporter is different for each construction.
    The first CsgA is called CsgA WT, the sequence is exactly the same as we found in a wild type CsgA protein except one mutation in order to eliminate one restriction site. The construction is also called CsgA-WT.
    The second CsgA is based on the same sequence but with a short sequence coding for a his tag(a peptide chain coding six histidines). This construction is called CsgA-His1.
    The last CsgA is almost the same but with 2 his tag. This construction is called CsgA-His2.

    • <groupparts>iGEM014 INSA-Lyon</groupparts>

    Retrieved from "http://2014.igem.org/Team:INSA-Lyon/Parts"