Team:Paris Bettencourt/Parts

From 2014.igem.org

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         <div id=project1><h6>Smell the roses</h6>
         <div id=project1><h6>Smell the roses</h6>
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         <div class=part id=part1><p><a href="http://parts.igem.org/Part:BBa_K1403003"><u>BBa_K1403003</a> - Limonene synthase</u></br></br>This part consists of a limonene synthase coding sequence downstream of a constitutive promoter.</br></br>Limonene syntheses enzyme converts farnesyl-diphosphate to (+)-limonene, which is a component of lemon scent./p></div>
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         <div class=part id=part1><p><a href="http://parts.igem.org/Part:BBa_K1403003"><u>BBa_K1403003</a> - Limonene synthase</u></br></br>Limonene syntheses enzyme converts farnesyl-diphosphate to (+)-limonene, which is a component of lemon scent./p></div>
         <div class=part id=part2><p><u><a href="http://parts.igem.org/Part:BBa_K1403006">BBa_K1403006</a> - Jasmonic acid carboxyl methyltransferase</u></br></br>Jasmonic acid carboxyl methyltransferase catalyzes the formation of methyl jasmonate from jasmonic acid</p></div>
         <div class=part id=part2><p><u><a href="http://parts.igem.org/Part:BBa_K1403006">BBa_K1403006</a> - Jasmonic acid carboxyl methyltransferase</u></br></br>Jasmonic acid carboxyl methyltransferase catalyzes the formation of methyl jasmonate from jasmonic acid</p></div>
  <div class=part id=part3><p><a href="http://parts.igem.org/Part:BBa_K1403009"><u>BBa_K1403009</a> - <i>bmst1</i></u></br></br> BMST1 enzyme that catalyzes production of methyl salicylate from salicylic acid. Methyl salicylate has a wintergreen smell.</p> </div>
  <div class=part id=part3><p><a href="http://parts.igem.org/Part:BBa_K1403009"><u>BBa_K1403009</a> - <i>bmst1</i></u></br></br> BMST1 enzyme that catalyzes production of methyl salicylate from salicylic acid. Methyl salicylate has a wintergreen smell.</p> </div>
         <div class=part id=part4><p><u><a href="http://parts.igem.org/Part:BBa_K1403012">BBa_K1403012</a> - alcohol acetyltransferase I</u></br></br>This enzyme converts isoamyl alcohol to isoamyl acetate producing banana odor</p></div>
         <div class=part id=part4><p><u><a href="http://parts.igem.org/Part:BBa_K1403012">BBa_K1403012</a> - alcohol acetyltransferase I</u></br></br>This enzyme converts isoamyl alcohol to isoamyl acetate producing banana odor</p></div>
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     <div class=part id=part5><p><a href="http://parts.igem.org/Part:BBa_K1403017"><u>BBa_K1403017</a> - <i>aldB</i></u></br></br>It stimulates acetolactate decarboxylase activities to transform acetolactate to acetoin, which smells like butter.</p></div>
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     <div class=part id=part5><p><a href="http://parts.igem.org/Part:BBa_K1403017"><u>BBa_K1403017</a> - <i>aldB</i></u></br></br>AldB stimulates acetolactate decarboxylase activities to transform acetolactate to acetoin, which smells like butter.</p></div>
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         <div class=part id=part6><p><a href="http://parts.igem.org/Part:BBa_K1403019"><u>BBa_K1403019</a> - <i>ilvBN</i></u></br></br><i>ilvBN</i> gene exists in natural wild type E.Coli strain. They codes for acetolactate synthase process. Here, we isolated it from MG1655 strain through PCR to transform it into targeted strain for increased basal production of acetolactate, which serves as precursor for acetoin production.</p></div>
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         <div class=part id=part6><p><a href="http://parts.igem.org/Part:BBa_K1403019"><u>BBa_K1403019</a> - <i>ilvBN</i></u></br></br><i>ilvBN</i> gene encodes for acetolactate synthase process. We used it to increase basal production of acetolactate, which serves as precursor for acetoin production.</p></div>
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         <div id=project2><h6>Something fishy</h6>
         <div id=project2><h6>Something fishy</h6>
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   <div class=part id=part9><p><u><a href="http://parts.igem.org/Part:BBa_K1403000">BBa_K1403000</a> - GFP under strong promoter in pSB1C3</u></br>Measurment kit test of Part:<a href="http://parts.igem.org/Part:BBa_J23101">BBa_J23101</a> in pSB1C3. This part is a tween of <a href="http://parts.igem.org/Part:BBa_I20260">BBa_I20260</a> but in a high copy pSB1C3 plasmid. </p></div>
   <div class=part id=part9><p><u><a href="http://parts.igem.org/Part:BBa_K1403000">BBa_K1403000</a> - GFP under strong promoter in pSB1C3</u></br>Measurment kit test of Part:<a href="http://parts.igem.org/Part:BBa_J23101">BBa_J23101</a> in pSB1C3. This part is a tween of <a href="http://parts.igem.org/Part:BBa_I20260">BBa_I20260</a> but in a high copy pSB1C3 plasmid. </p></div>
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  <div class=part id=part10><p><u><a href="http://parts.igem.org/Part:BBa_J23101">BBa_J23101</a>BBa_K1403000</a> - GFP generator under a weak promoter in pSB1C3</u></br>Measurment kit test of <a href="http://parts.igem.org/Part:BBa_J23115">BBa_J23115</a> in pSB1C3 or more precisely of <a href="http://parts.igem.org/Part:BBa_K823012">BBa_K823012</a> becuse it has two mismaches compared to <a href="http://parts.igem.org/Part:BBa_J23115">BBa_J23115</a> . Placed in a high coply plasmid,the GFP expression is under a weak Anderson promoter. </p></div>
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  <div class=part id=part10><p><u><a href="http://parts.igem.org/Part:BBa_J23101">BBa_J23101</a>BBa_K1403000</a> - GFP generator under a weak promoter in pSB1C3</u></br>Measurment kit test of <a href="http://parts.igem.org/Part:BBa_J23115">BBa_J23115</a> in pSB1C3. It is placed in a high coply plasmid,the GFP expression is under a weak Anderson promoter.</p></div>
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Revision as of 16:49, 17 October 2014

SMELL THE ROSES

SOMETHING FISHY


DON'T SWEAT IT

INTERLAB STUDY

Smell the roses

BBa_K1403003 - Limonene synthase

Limonene syntheses enzyme converts farnesyl-diphosphate to (+)-limonene, which is a component of lemon scent./p>

BBa_K1403006 - Jasmonic acid carboxyl methyltransferase

Jasmonic acid carboxyl methyltransferase catalyzes the formation of methyl jasmonate from jasmonic acid

BBa_K1403009 - bmst1

BMST1 enzyme that catalyzes production of methyl salicylate from salicylic acid. Methyl salicylate has a wintergreen smell.

BBa_K1403012 - alcohol acetyltransferase I

This enzyme converts isoamyl alcohol to isoamyl acetate producing banana odor

BBa_K1403017 - aldB

AldB stimulates acetolactate decarboxylase activities to transform acetolactate to acetoin, which smells like butter.

BBa_K1403019 - ilvBN

ilvBN gene encodes for acetolactate synthase process. We used it to increase basal production of acetolactate, which serves as precursor for acetoin production.

Something fishy

BBa_K1403015 - Trimethylamine monooxygenase
Trimethylamine monooxygenase enzyme degrades Trimethylamine (fish odor) into non-smelly compund. It also produces indigo (blue pigment) form tryptophan.

Don't sweat it

BBa_K1403018 - agaA
agaA is a gene present in Corynebacterium striatum and it is the main responsible for body odor. It transforms 3-methyl-2-hexenoic acid into 3-hydroxy-3-methylhexanoic acid.

Interlab study

BBa_K1403000 - GFP under strong promoter in pSB1C3
Measurment kit test of Part:BBa_J23101 in pSB1C3. This part is a tween of BBa_I20260 but in a high copy pSB1C3 plasmid.

BBa_J23101BBa_K1403000 - GFP generator under a weak promoter in pSB1C3
Measurment kit test of BBa_J23115 in pSB1C3. It is placed in a high coply plasmid,the GFP expression is under a weak Anderson promoter.

Centre for Research and Interdisciplinarity (CRI)
Faculty of Medicine Cochin Port-Royal, South wing, 2nd floor
Paris Descartes University
24, rue du Faubourg Saint Jacques
75014 Paris, France
+33 1 44 41 25 22/25
paris-bettencourt-igem@googlegroups.com
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