"
Team:Paris Bettencourt/Parts
From 2014.igem.org
| Line 179: | Line 179: | ||
<div class=part id=part9><p><u><a href="http://parts.igem.org/Part:BBa_K1403000">BBa_K1403000</a> - GFP under strong promoter in pSB1C3</u></br>Measurment kit test of Part:<a href="http://parts.igem.org/Part:BBa_J23101">BBa_J23101</a> in pSB1C3. This part is a tween of <a href="http://parts.igem.org/Part:BBa_I20260">BBa_I20260</a> but in a high copy pSB1C3 plasmid. </p></div> | <div class=part id=part9><p><u><a href="http://parts.igem.org/Part:BBa_K1403000">BBa_K1403000</a> - GFP under strong promoter in pSB1C3</u></br>Measurment kit test of Part:<a href="http://parts.igem.org/Part:BBa_J23101">BBa_J23101</a> in pSB1C3. This part is a tween of <a href="http://parts.igem.org/Part:BBa_I20260">BBa_I20260</a> but in a high copy pSB1C3 plasmid. </p></div> | ||
</div> | </div> | ||
| - | <div class=part id=part10><p>Part | + | <div class=part id=part10><p><a href="http://parts.igem.org/Part:BBa_J23101">BBa_J23101</a>BBa_K1403000</a> - GFP generator under a weak promoter in pSB1C3</br>Measurment kit test of <a href="http://parts.igem.org/Part:BBa_J23115">BBa_J23115</a> in pSB1C3 or more precisely of <a href="http://parts.igem.org/Part:BBa_K823012">BBa_K823012</a> becuse it has two mismaches compared to <a href="http://parts.igem.org/Part:BBa_J23115">BBa_J23115</a> . Placed in a high coply plasmid,the GFP expression is under a weak Anderson promoter. </p></div> |
</div> | </div> | ||
| - | </body> | + | </body> |
</html> | </html> | ||
{{:Team:Paris_Bettencourt/Footer}} | {{:Team:Paris_Bettencourt/Footer}} | ||
Revision as of 16:43, 17 October 2014
| SMELL THE ROSES | SOMETHING FISHY | DON'T SWEAT IT | INTERLAB STUDY |
Smell the roses
BBa_K1403003 - Limonene synthaseThis part consists of a limonene synthase coding sequence downstream of a constitutive promoter.Limonene syntheses enzyme converts farnesyl-diphosphate to (+)-limonene, which is a component of lemon scent./p>
BBa_K1403006 - Jasmonic acid carboxyl methyltransferaseJasmonic acid carboxyl methyltransferase catalyzes the formation of methyl jasmonate from jasmonic acid
BBa_K1403009 - bmst1 BMST1 enzyme that catalyzes production of methyl salicylate from salicylic acid. Methyl salicylate has a wintergreen smell.
BBa_K1403012 - alcohol acetyltransferase IThis enzyme converts isoamyl alcohol to isoamyl acetate producing banana odor
BBa_K1403017 - aldBIt stimulates acetolactate decarboxylase activities to transform acetolactate to acetoin, which smells like butter.
BBa_K1403019 - ilvBNilvBN gene exists in natural wild type E.Coli strain. They codes for acetolactate synthase process. Here, we isolated it from MG1655 strain through PCR to transform it into targeted strain for increased basal production of acetolactate, which serves as precursor for acetoin production.
Something fishy
BBa_K1403015 - Trimethylamine monooxygenaseTrimethylamine monooxygenase enzyme degrades Trimethylamine (fish odor) into non-smelly compund. It also produces indigo (blue pigment) form tryptophan.
Don't sweat it
BBa_K1403018 - agaAagaA is a gene present in Corynebacterium striatum and it is the main responsible for body odor. It transforms 3-methyl-2-hexenoic acid into 3-hydroxy-3-methylhexanoic acid.
Interlab study
BBa_K1403000 - GFP under strong promoter in pSB1C3Measurment kit test of Part:BBa_J23101 in pSB1C3. This part is a tween of BBa_I20260 but in a high copy pSB1C3 plasmid.
BBa_J23101BBa_K1403000 - GFP generator under a weak promoter in pSB1C3Measurment kit test of BBa_J23115 in pSB1C3 or more precisely of BBa_K823012 becuse it has two mismaches compared to BBa_J23115 . Placed in a high coply plasmid,the GFP expression is under a weak Anderson promoter.
+33 1 44 41 25 22/25
