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Team:Groningen/Template/MODULE/Notebook/characterisation/week2
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Lisahielkema (Talk | contribs) (Created page with "<html> <!--Content module--> <div class="module contentmodule gridcell"> <div class="content"> <div class="wrapper"> <!-- TITLE SNIPPET START--> <div class="title"> 8 - 14 Sep...") |
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| - | Obtaining | + | Obtaining the NisA (BBa_K1365000) and Superfolded GFP (BBa_K1365020) BioBrick in larger concentration for further use |
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| - | <div class="item">Transformed | + | <div class="item">Transformed both, pSB1C3 containing the NisA BioBrick (BBa_K1365000) and pSB1C3 with the Superfolded GFP Biobrick (BBa_K1365020) to two different <i>E.col</i> DH5α strains</div> |
| - | <div class="item"> | + | <div class="item">The transformations were checked on gel, and the desired bands were observed, therefore the transformation was most likely successful</div> |
| - | <div class="item"> | + | <div class="item">The plasmids with the desired BioBricks were isolated out of <i>E. coli</i> DH5α, and glycerol stocks were made of the two strains containing the previous named parts</div> |
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Revision as of 16:17, 17 October 2014
8 - 14 September
Obtaining the NisA (BBa_K1365000) and Superfolded GFP (BBa_K1365020) BioBrick in larger concentration for further use
Transformed both, pSB1C3 containing the NisA BioBrick (BBa_K1365000) and pSB1C3 with the Superfolded GFP Biobrick (BBa_K1365020) to two different E.col DH5α strains
The transformations were checked on gel, and the desired bands were observed, therefore the transformation was most likely successful
The plasmids with the desired BioBricks were isolated out of E. coli DH5α, and glycerol stocks were made of the two strains containing the previous named parts
