Team:Groningen/Template/MODULE/Notebook/toolbox/appendix/table3

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(Difference between revisions)

Latest revision as of 16:00, 17 October 2014

Primers for making BioBricks out of genes with illegal restriction sites

The primers are denoted with the position of the illegal restriction site that they should remove. Illegal EcoRI sites are abbreviated with an E, XbaI sites with an X, SpeI site with an S and PstI sites with a P.

Gene	Primer	Sequence
NisT	Prefix forward	GTTTCTTCGAATTCGCGGCCGCTTCTAGATGGATGAAGTGAAAGAATTTACATCA
	S980 reverse	TTGTTCCATAAACAAACTTGTATTATAAATGATGTAAATA
	S980 forward	TATTTACATCATTTATAATACAAGTTTGTTTATGGAACAA
	S1210 reverse	CCATAGTTGGTTGATATAATCCTGAAATTATCTTTACTTGTGTAC
	S1210 forward	GTACACAAGTAAAGATAATTTCAGGATTATATCAACCAACTATGG
	Suffix reverse	GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTATTCATCATTATCCT
	Suffix forward	ATGAATAATAATACTAGTAGCGGCCGCTGCAGGAAGAAACAAAAGGGCAA
	Prefix reverse	CACTTCATCCATCTAGAAGCGGCCGCGAATTCGAAGAAACATCCTTAGCG
NisP	Prefix forward	GTTTCTTCGAATTCGCGGCCGCTTCTAGGTGAAAAAAATACTAGGTTT
	P225 reverse	AGTCCTTGTTGTCGATTTTACTGCTGGTGACTGCGCCCCT
	P225 forward	AGGGGCGCAGTCACCAGCAGTAAAATCGACAACAAGGACT
	E347 reverse	AACGCTATCTCTCTTACTAAATTCAGAACTAACTTGAGTT
	E347 forward	AACTCAAGTTAGTTCTGAATTTAGTAAGAGAGATAGCGTT
	E1657 reverse	CAATACTCTATTCCCATTAACTTCTGGACTATTCATTAGA
	E1657 forward	TCTAATGAATAGTCCAGAAGTTAATGGGAATAGAGTATTG
	Suffix reverse	GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATCAATTTTTAGTCTTTC
	Suffix forward	AAATTGATAATACTAGTAGCGGCCGCTGCAGGAAGAAACAAAAGGGCAA
	Prefix reverse	CCTAGTATTTTTTTCATCTAGAAGCGGCCGCGAATTCGAAGAAACATCCTTAGCG
NisFEG	Prefix forward	GTTTCTTCGAATTCGCGGCCGCTTCTAGATGCAGGTAAAAATTCAAAA
	P186 reverse	TCCAGTATCAGCAGAAATTAAACCAAACAAAATTTTCATC
	P186 forward	GATGAAAATTTTGTTTGGTTTAATTTCTGCTGATACTGGA
	E336/X349 reverse	CCTGTTTCTGCCAAACCAATCACTTCTAGTGTTTCATGTATTCTCTTA
	E336/X349 forward	TAAGAGAATACATGAAACACTAGAAGTGATTGGTTTGGCAGAAACAGG
	Suffix reverse	GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTATCTAATCTTTTTTT
	Suffix forward	TAGATAATAATACTAGTAGCGGCCGCTGCAGGAAGAAACAAAAGGGCAA
	Prefix reverse	TTTTACCTGCATCTAGAAGCGGCCGCGAATTCGAAGAAACATCCTTAGCG

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+<!-- CAPTION OPTIONAL START-->
+<div class="caption">
+The primers are denoted with the position of the
+illegal restriction site that they should remove. Illegal EcoRI sites
+are abbreviated with an E, XbaI sites with an X, SpeI site with an S and
+PstI sites with a P.
+</div>
+<div class="hspacer">&nbsp;</div>
+<!-- CAPTION OPTIONAL END-->
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@@ Line 25: / Line 34: @@
    </tr>
    <tr>
-     <td rowspan="8">NisT</td>
+     <td>NisT</td>
      <td>Prefix forward</td>
      <td>GTTTCTTCGAATTCGCGGCCGCTTCTAGATGGATGAAGTGAAAGAATTTACATCA</td>
    </tr>
    <tr>
+    <td>&nbsp;</td>
      <td>S980 reverse</td>
      <td>TTGTTCCATAAACAAACTTGTATTATAAATGATGTAAATA</td>
    </tr>
    <tr>
+    <td>&nbsp;</td>
      <td>S980 forward</td>
      <td>TATTTACATCATTTATAATACAAGTTTGTTTATGGAACAA</td>
    </tr>
    <tr>
+    <td>&nbsp;</td>
      <td>S1210 reverse</td>
      <td>CCATAGTTGGTTGATATAATCCTGAAATTATCTTTACTTGTGTAC</td>
    </tr>
    <tr>
+    <td>&nbsp;</td>
      <td>S1210 forward</td>
      <td>GTACACAAGTAAAGATAATTTCAGGATTATATCAACCAACTATGG</td>
    </tr>
    <tr>
+    <td>&nbsp;</td>
      <td>Suffix reverse</td>
      <td>GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTATTCATCATTATCCT</td>
    </tr>
    <tr>
+    <td>&nbsp;</td>
      <td>Suffix forward</td>
      <td>ATGAATAATAATACTAGTAGCGGCCGCTGCAGGAAGAAACAAAAGGGCAA</td>
    </tr>
    <tr>
+    <td>&nbsp;</td>
      <td>Prefix reverse</td>
      <td>CACTTCATCCATCTAGAAGCGGCCGCGAATTCGAAGAAACATCCTTAGCG</td>
    </tr>
    <tr>
-     <td rowspan="10">NisP</td>
+     <td>NisP</td>
      <td>Prefix forward</td>
      <td>GTTTCTTCGAATTCGCGGCCGCTTCTAGGTGAAAAAAATACTAGGTTT</td>
    <tr>
+    <td>&nbsp;</td>
      <td>P225 reverse</td>
      <td>AGTCCTTGTTGTCGATTTTACTGCTGGTGACTGCGCCCCT</td>
    </tr>
    <tr>
+    <td>&nbsp;</td>
      <td>P225 forward</td>
      <td>AGGGGCGCAGTCACCAGCAGTAAAATCGACAACAAGGACT</td>
    </tr>
    <tr>
+    <td>&nbsp;</td>
      <td>E347 reverse</td>
      <td>AACGCTATCTCTCTTACTAAATTCAGAACTAACTTGAGTT</td>
    </tr>
    <tr>
+    <td>&nbsp;</td>
      <td>E347 forward</td>
      <td>AACTCAAGTTAGTTCTGAATTTAGTAAGAGAGATAGCGTT</td>
    </tr>
    <tr>
+    <td>&nbsp;</td>
      <td>E1657 reverse</td>
      <td>CAATACTCTATTCCCATTAACTTCTGGACTATTCATTAGA</td>
    </tr>
    <tr>
+    <td>&nbsp;</td>
      <td>E1657 forward</td>
      <td>TCTAATGAATAGTCCAGAAGTTAATGGGAATAGAGTATTG</td>
    </tr>
    <tr>
+    <td>&nbsp;</td>
      <td>Suffix reverse</td>
      <td>GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATCAATTTTTAGTCTTTC</td>
    </tr>
    <tr>
+    <td>&nbsp;</td>
      <td>Suffix forward</td>
      <td>AAATTGATAATACTAGTAGCGGCCGCTGCAGGAAGAAACAAAAGGGCAA</td>
    </tr>
    <tr>
+    <td>&nbsp;</td>
      <td>Prefix reverse</td>
      <td>CCTAGTATTTTTTTCATCTAGAAGCGGCCGCGAATTCGAAGAAACATCCTTAGCG</td>
    </tr>
    <tr>
-     <td rowspan="8">NisFEG</td>
+     <td>NisFEG</td>
      <td>Prefix forward</td>
      <td>GTTTCTTCGAATTCGCGGCCGCTTCTAGATGCAGGTAAAAATTCAAAA</td>
    </tr>
    <tr>
+    <td>&nbsp;</td>
      <td>P186 reverse</td>
      <td>TCCAGTATCAGCAGAAATTAAACCAAACAAAATTTTCATC</td>
    </tr>
    <tr>
+    <td>&nbsp;</td>
      <td>P186 forward</td>
      <td>GATGAAAATTTTGTTTGGTTTAATTTCTGCTGATACTGGA</td>
    </tr>
    <tr>
+    <td>&nbsp;</td>
      <td>E336/X349 reverse</td>
      <td>CCTGTTTCTGCCAAACCAATCACTTCTAGTGTTTCATGTATTCTCTTA</td>
    </tr>
    <tr>
+    <td>&nbsp;</td>
      <td>E336/X349 forward</td>
      <td>TAAGAGAATACATGAAACACTAGAAGTGATTGGTTTGGCAGAAACAGG</td>
    </tr>
-      <tr>
+  <tr>
+    <td>&nbsp;</td>
      <td>Suffix reverse</td>
      <td>GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTATCTAATCTTTTTTT</td>
    </tr>
    </tr>
-      <tr>
+  <tr>
+    <td>&nbsp;</td>
      <td>Suffix forward</td>
      <td>TAGATAATAATACTAGTAGCGGCCGCTGCAGGAAGAAACAAAAGGGCAA</td>
    </tr>
    <tr>
+    <td>&nbsp;</td>
      <td>Prefix reverse</td>
      <td>TTTTACCTGCATCTAGAAGCGGCCGCGAATTCGAAGAAACATCCTTAGCG</td>
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