"
Team:Cambridge-JIC/Guide/Transformation/Outline
From 2014.igem.org
(Difference between revisions)
(→Outline) |
|||
| (One intermediate revision not shown) | |||
| Line 1: | Line 1: | ||
| + | {{:Team:Cambridge-JIC/Templates/header_prototype3}} | ||
=Outline= | =Outline= | ||
Agrobacterium mediated transformation is extensively used in many plant and fungal chassis. For Marchantia, two main protocols have been developed to optimise speed and efficiency of transformation: | Agrobacterium mediated transformation is extensively used in many plant and fungal chassis. For Marchantia, two main protocols have been developed to optimise speed and efficiency of transformation: | ||
| - | |||
| - | |||
| - | -[[Team:Cambridge-JIC/Guide/Transformation/AgarTrap| agar trap]] method. This requires only a ventilated space under full illumination at room temperature. | + | -the [[Team:Cambridge-JIC/Guide/Transformation/Cocultivation| cocultivation]] method. This requires a shaker capable of shaking a 100ml conical flask at 130rpm under full illumination. |
| + | |||
| + | -the [[Team:Cambridge-JIC/Guide/Transformation/AgarTrap| agar trap]] method. This requires only a ventilated space under full illumination at room temperature. | ||
==Timing== | ==Timing== | ||
| Line 12: | Line 13: | ||
'''For each method, your timing will be optimal if you [[Team:Cambridge-JIC/Guide/Care/PreparingSpores| prepare spores]] and [[Team:Cambridge-JIC/Guide/Transformation/TransformingAgro| transform agrobacteria]] on day 1. | '''For each method, your timing will be optimal if you [[Team:Cambridge-JIC/Guide/Care/PreparingSpores| prepare spores]] and [[Team:Cambridge-JIC/Guide/Transformation/TransformingAgro| transform agrobacteria]] on day 1. | ||
''' | ''' | ||
| + | <html><br><br><br><br><br><br></html> | ||
Latest revision as of 15:34, 17 October 2014
Outline
Agrobacterium mediated transformation is extensively used in many plant and fungal chassis. For Marchantia, two main protocols have been developed to optimise speed and efficiency of transformation:
-the cocultivation method. This requires a shaker capable of shaking a 100ml conical flask at 130rpm under full illumination.
-the agar trap method. This requires only a ventilated space under full illumination at room temperature.
Timing
The cocultivation method requires spores to be (at least) 5 days old prior to cocultivation with agrobacterium, and the agar trap 3 days old. Agrobacteria takes 3 days to grow after transformation.
For each method, your timing will be optimal if you prepare spores and transform agrobacteria on day 1.
