Team:Tuebingen/Notebook/Protocols/TAE buffer

From 2014.igem.org

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<h1>Protocols</h1>
<h3>TAE buffer</h3>
<h3>TAE buffer</h3>
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<h4>Reagents</h4>
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<table border="0">
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  <colgroup>
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    <col width="100">
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    <col width="300">
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  </colgroup>
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  <tr>
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    <td style="text-align: center">242 g</td>
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    <td>Tris</td>
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  </tr>
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  <tr>
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    <td style="text-align: center">57.1 mL</td>
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    <td>pure acetic acid</td>
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  </tr>
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  <tr>
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    <td style="text-align: center">0.5 M</td>
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    <td>EDTA 0.5 M pH 8.0</td>
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  </tr>
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</table>
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<p>&nbsp;</p>
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<h4>Procedure</h4>
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<p>Mix all the reagents and add to 1000 ml with distillated water. Adjust to pH 8.3. Store at room temperature.</p>

Latest revision as of 15:27, 17 October 2014


Protocols

TAE buffer

Reagents

242 g Tris
57.1 mL pure acetic acid
0.5 M EDTA 0.5 M pH 8.0

 

Procedure

Mix all the reagents and add to 1000 ml with distillated water. Adjust to pH 8.3. Store at room temperature.

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