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Team:Tuebingen/Notebook/Protocols/PCR
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| - | + | <h1>Protocols</h1> | |
<h3>Polymerase chain reaction</h3> | <h3>Polymerase chain reaction</h3> | ||
| + | <h4>Reagents</h4> | ||
| + | <table border="0"> | ||
| + | <colgroup> | ||
| + | <col width="100"> | ||
| + | <col width="300"> | ||
| + | </colgroup> | ||
| + | |||
| + | <tr> | ||
| + | <td style="text-align: center"> </td> | ||
| + | <td>Template (10-100 ng)</td> | ||
| + | </tr> | ||
| + | |||
| + | <tr> | ||
| + | <td style="text-align: center">1 µL</td> | ||
| + | <td>Primer forward (10 µM)</td> | ||
| + | </tr> | ||
| + | |||
| + | <tr> | ||
| + | <td style="text-align: center">1 µL</td> | ||
| + | <td>Primer reverse (10 µM)</td> | ||
| + | </tr> | ||
| + | |||
| + | <tr> | ||
| + | <td style="text-align: center">1 µL</td> | ||
| + | <td>dNTP mix (10 mM)</td> | ||
| + | </tr> | ||
| + | |||
| + | <tr> | ||
| + | <td style="text-align: center">5 µL</td> | ||
| + | <td>10x PCR buffer</td> | ||
| + | </tr> | ||
| + | |||
| + | <tr> | ||
| + | <td style="text-align: center">0.5 µL</td> | ||
| + | <td>Pfu Polymerase (1 U/µL)</td> | ||
| + | </tr> | ||
| + | |||
| + | <tr> | ||
| + | <td style="text-align: center">to 50 µL</td> | ||
| + | <td>H20</td> | ||
| + | </tr> | ||
| + | </table> | ||
| + | |||
| + | <p> </p> | ||
| + | |||
| + | <h4>Procedure</h4> | ||
| + | <p>The reagents are mixed in a PCR-tube in which the polymerase is added last. Run the cycler | ||
| + | |||
| + | using the appropriate program for the used polymerases and primers.</p> | ||
Latest revision as of 15:26, 17 October 2014
Protocols
Polymerase chain reaction
Reagents
| Template (10-100 ng) | |
| 1 µL | Primer forward (10 µM) |
| 1 µL | Primer reverse (10 µM) |
| 1 µL | dNTP mix (10 mM) |
| 5 µL | 10x PCR buffer |
| 0.5 µL | Pfu Polymerase (1 U/µL) |
| to 50 µL | H20 |
Procedure
The reagents are mixed in a PCR-tube in which the polymerase is added last. Run the cycler using the appropriate program for the used polymerases and primers.
