Team:UT-Dallas/Notebook/8-28

From 2014.igem.org

(Difference between revisions)
(Created page with "{{CSS/Main}} <html xmlns="http://www.w3.org/1999/xhtml" xml:lang="en" lang="en" dir="ltr"> <h1 class="firstHeading" align="center">Sunday, August 28th, 2014</h1> <center><p><a h...")
 
(3 intermediate revisions not shown)
Line 1: Line 1:
{{CSS/Main}}
{{CSS/Main}}
<html xmlns="http://www.w3.org/1999/xhtml" xml:lang="en" lang="en" dir="ltr">
<html xmlns="http://www.w3.org/1999/xhtml" xml:lang="en" lang="en" dir="ltr">
 +
<!--navigation menu -->
 +
<table align="center">
 +
<td align="center" colspan="12">
-
<h1 class="firstHeading" align="center">Sunday, August 28th, 2014</h1>
+
<table  width="100%">
 +
<tr height="15px"></tr>
 +
<tr height="75px">
 +
 
 +
<td style="display: inline; padding: 15px; text-decoration: none; " align="center" height ="45px" onMouseOver="this.bgColor='#4E817B'" onMouseOut="this.bgColor='#70B9B0'" bgColor=#70B9B0> 
 +
<a href="https://2014.igem.org/Team:UT-Dallas"style="color:#ffffff; text-decoration: none; text-transform: uppercase; font-weight: bold;" >Home </a> </td>
 +
 
 +
<td style="display: inline; padding: 15px; text-decoration: none;" align="center" height ="45px" onMouseOver="this.bgColor='#4E817B'" onMouseOut="this.bgColor='#70B9B0'" bgColor=#70B9B0>
 +
<a href="https://2014.igem.org/Team:UT-Dallas/Team"style="color:#ffffff; text-decoration: none; text-transform: uppercase; font-weight: bold;"> Team </a> </td>
 +
 
 +
<td style="display: inline; padding: 15px; text-decoration: none;" align="center"  height ="45px"  onMouseOver="this.bgColor='#4E817B'" onMouseOut="this.bgColor='#70B9B0'" bgColor=#70B9B0>
 +
<a href="https://igem.org/Team.cgi?year=2014&team_name=UT-Dallas"style="color:#ffffff; text-decoration: none; text-transform: uppercase; font-weight: bold;"> Official Profile </a></td>
 +
 
 +
<td style="display: inline; padding: 15px; text-decoration: none;" align="center"  height ="45px" onMouseOver="this.bgColor='#4E817B'" onMouseOut="this.bgColor='#70B9B0'" bgColor=#70B9B0> 
 +
<a href="https://2014.igem.org/Team:UT-Dallas/Project"style="color:#ffffff; text-decoration: none; text-transform: uppercase; font-weight: bold;"> Project</a></td>
 +
 
 +
<td style="display: inline; padding: 15px; text-decoration: none;" align="center"  height ="45px" onMouseOver="this.bgColor='#4E817B'" onMouseOut="this.bgColor='#70B9B0'" bgColor=#70B9B0>
 +
<a href="https://2014.igem.org/Team:UT-Dallas/Parts"style="color:#ffffff; text-decoration: none; text-transform: uppercase; font-weight: bold;"> Parts</a></td>
 +
 
 +
<td style="display: inline; padding: 15px; text-decoration: none;" align="center" height ="45px" onMouseOver="this.bgColor='#4E817B'" onMouseOut="this.bgColor='#70B9B0'" bgColor=#70B9B0>
 +
<a href="https://2014.igem.org/Team:UT-Dallas/Modeling"style="color:#ffffff; text-decoration: none; text-transform: uppercase; font-weight: bold;"> Modeling</a></td>
 +
 
 +
<td style="display: inline; padding: 15px; text-decoration: none;" align="center" height ="45px" onMouseOver="this.bgColor='#4E817B'" onMouseOut="this.bgColor='#70B9B0'" bgColor=#70B9B0> 
 +
<a href="https://2014.igem.org/Team:UT-Dallas/Notebook"style="color:#ffffff; text-decoration: none; text-transform: uppercase; font-weight: bold;"> Notebook</a></td>
 +
 
 +
<td style="display: inline; padding: 15px; text-decoration: none;" align="center"  height ="45px" onMouseOver="this.bgColor='#4E817B'" onMouseOut="this.bgColor='#70B9B0'" bgColor=#70B9B0>
 +
<a href="https://2014.igem.org/Team:UT-Dallas/Safety"style=" color:#ffffff; text-decoration: none; text-transform: uppercase; font-weight: bold;"> Safety </a></td>
 +
 
 +
<td style="display: inline; padding: 15px; text-decoration: none;" align="center"  height ="45px" onMouseOver="this.bgColor='#4E817B'" onMouseOut="this.bgColor='#70B9B0'" bgColor=#70B9B0>
 +
<a href="https://2014.igem.org/Team:UT-Dallas/Attributions"style="color:#ffffff; text-decoration: none; text-transform: uppercase; font-weight: bold;"> Attributions </a></td>
 +
 
 +
<td style="display: inline; padding: 15px; text-decoration: none;" align="center"  height ="45px" onMouseOver="this.bgColor='#4E817B'" onMouseOut="this.bgColor='#70B9B0'" bgColor=#70B9B0>
 +
<a href="https://2014.igem.org/Team:UT-Dallas/Human-Practices"style="color:#ffffff; text-decoration: none; text-transform: uppercase; font-weight: bold;"> Human Practices</a></td>
 +
 
 +
 
 +
<td style="display: inline; padding: 5px;" align ="center"> <a href="https://2014.igem.org/Team:UT-Dallas"> <img src="https://static.igem.org/mediawiki/2014/e/e1/UTDmodifiedlogo.jpg" height="45px"></a></td>
 +
<td style="display: inline; padding: 5px;" align ="center"> <a href="https://2014.igem.org/Main_Page"> <img src="https://static.igem.org/mediawiki/igem.org/6/60/Igemlogo_300px.png" width="55px"></a> </td>
 +
 
 +
</tr>
 +
</table>
 +
</tr>
 +
 
 +
 
 +
</tr>
 +
 +
 
 +
</td>
 +
 
 +
<tr> <td colspan="3"  height="15px"> </td></tr>
 +
<tr><td bgColor="#e7e7e7" colspan="3" height="1px"> </tr>
 +
<tr> <td colspan="3"  height="5px"> </td></tr>
 +
</table>
 +
 
 +
<!------end nav-->
 +
 
 +
<h1 class="firstHeading" align="center">Thursday, August 28th, 2014</h1>
<center><p><a href="https://2014.igem.org/wiki/index.php?title=Team:UT-Dallas/Notebook/8-28&action=edit" "style="color:#87A96B" >Click here to edit the page </a> </p></center>
<center><p><a href="https://2014.igem.org/wiki/index.php?title=Team:UT-Dallas/Notebook/8-28&action=edit" "style="color:#87A96B" >Click here to edit the page </a> </p></center>
Line 33: Line 91:
<LI> Made glycerol stock and Miniprepped clones.  
<LI> Made glycerol stock and Miniprepped clones.  
           <UL>
           <UL>
-
           <LI> Carb Reporter Vector clones: acfA (2), acfB (2), ctxA (2), tcpT (2)
+
           <LI> Carb Reporter Vector clones: tcpB (2), tcpF (2)
            
            
           </UL>
           </UL>
<LI> Nanodrop to measure DNA concentration.
<LI> Nanodrop to measure DNA concentration.
-
 
+
        <LI> Inoculated colonies from 8/27 Transformation of Carb Reporters in Carb broth.
 +
        <UL>
 +
        <LI> acfA (2), acfB(2), ctxA (2), tcpT (2)
</UL>
</UL>
       </td>
       </td>
Line 53: Line 113:
  <hr width="100%" align="center">
  <hr width="100%" align="center">
   <p>
   <p>
-
       <img src="http://i.imgur.com/tvZ5mTn.jpg" height="400px">
+
       <img src="https://static.igem.org/mediawiki/2014/thumb/e/e0/UTDallasAugust28i.jpg/800px-UTDallasAugust28i.jpg" height="400px">
-
       <br>  
+
       <br> Lab Preperation
       <div class="gallerylayer" opacity: 1; background: white;">
       <div class="gallerylayer" opacity: 1; background: white;">
        
        

Latest revision as of 14:41, 17 October 2014

Home Team Official Profile Project Parts Modeling Notebook Safety Attributions Human Practices

Thursday, August 28th, 2014

Click here to edit the page


Purified plasmid DNA from Carb Resistant Vector clones for testing later next week.

Today's tasks:

  • Made glycerol stock and Miniprepped clones.
    • Carb Reporter Vector clones: tcpB (2), tcpF (2)
  • Nanodrop to measure DNA concentration.
  • Inoculated colonies from 8/27 Transformation of Carb Reporters in Carb broth.
    • acfA (2), acfB(2), ctxA (2), tcpT (2)



Lab Preperation