Team:UT-Dallas/Notebook/8-24

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{{CSS/Main}}
{{CSS/Main}}
<html xmlns="http://www.w3.org/1999/xhtml" xml:lang="en" lang="en" dir="ltr">
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<td style="display: inline; padding: 15px; text-decoration: none; " align="center" height ="45px" onMouseOver="this.bgColor='#4E817B'" onMouseOut="this.bgColor='#70B9B0'" bgColor=#70B9B0> 
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<a href="https://2014.igem.org/Team:UT-Dallas"style="color:#ffffff; text-decoration: none; text-transform: uppercase; font-weight: bold;" >Home </a> </td>
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<td style="display: inline; padding: 15px; text-decoration: none;" align="center" height ="45px" onMouseOver="this.bgColor='#4E817B'" onMouseOut="this.bgColor='#70B9B0'" bgColor=#70B9B0>
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<a href="https://2014.igem.org/Team:UT-Dallas/Team"style="color:#ffffff; text-decoration: none; text-transform: uppercase; font-weight: bold;"> Team </a> </td>
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<a href="https://igem.org/Team.cgi?year=2014&team_name=UT-Dallas"style="color:#ffffff; text-decoration: none; text-transform: uppercase; font-weight: bold;"> Official Profile </a></td>
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<td style="display: inline; padding: 15px; text-decoration: none;" align="center"  height ="45px" onMouseOver="this.bgColor='#4E817B'" onMouseOut="this.bgColor='#70B9B0'" bgColor=#70B9B0> 
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<a href="https://2014.igem.org/Team:UT-Dallas/Project"style="color:#ffffff; text-decoration: none; text-transform: uppercase; font-weight: bold;"> Project</a></td>
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<a href="https://2014.igem.org/Team:UT-Dallas/Parts"style="color:#ffffff; text-decoration: none; text-transform: uppercase; font-weight: bold;"> Parts</a></td>
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<td style="display: inline; padding: 15px; text-decoration: none;" align="center" height ="45px" onMouseOver="this.bgColor='#4E817B'" onMouseOut="this.bgColor='#70B9B0'" bgColor=#70B9B0>
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<a href="https://2014.igem.org/Team:UT-Dallas/Modeling"style="color:#ffffff; text-decoration: none; text-transform: uppercase; font-weight: bold;"> Modeling</a></td>
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<td style="display: inline; padding: 15px; text-decoration: none;" align="center" height ="45px" onMouseOver="this.bgColor='#4E817B'" onMouseOut="this.bgColor='#70B9B0'" bgColor=#70B9B0> 
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<a href="https://2014.igem.org/Team:UT-Dallas/Notebook"style="color:#ffffff; text-decoration: none; text-transform: uppercase; font-weight: bold;"> Notebook</a></td>
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<td style="display: inline; padding: 15px; text-decoration: none;" align="center"  height ="45px" onMouseOver="this.bgColor='#4E817B'" onMouseOut="this.bgColor='#70B9B0'" bgColor=#70B9B0>
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<a href="https://2014.igem.org/Team:UT-Dallas/Safety"style=" color:#ffffff; text-decoration: none; text-transform: uppercase; font-weight: bold;"> Safety </a></td>
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<td style="display: inline; padding: 15px; text-decoration: none;" align="center"  height ="45px" onMouseOver="this.bgColor='#4E817B'" onMouseOut="this.bgColor='#70B9B0'" bgColor=#70B9B0>
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<a href="https://2014.igem.org/Team:UT-Dallas/Attributions"style="color:#ffffff; text-decoration: none; text-transform: uppercase; font-weight: bold;"> Attributions </a></td>
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<a href="https://2014.igem.org/Team:UT-Dallas/Human-Practices"style="color:#ffffff; text-decoration: none; text-transform: uppercase; font-weight: bold;"> Human Practices</a></td>
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<td style="display: inline; padding: 5px;" align ="center"> <a href="https://2014.igem.org/Team:UT-Dallas"> <img src="https://static.igem.org/mediawiki/2014/e/e1/UTDmodifiedlogo.jpg" height="45px"></a></td>
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<td style="display: inline; padding: 5px;" align ="center"> <a href="https://2014.igem.org/Main_Page"> <img src="https://static.igem.org/mediawiki/igem.org/6/60/Igemlogo_300px.png" width="55px"></a> </td>
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<!------end nav-->
<h1 class="firstHeading" align="center">Sunday, August 24th, 2014</h1>
<h1 class="firstHeading" align="center">Sunday, August 24th, 2014</h1>
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   <p> Purified plasmid DNA from gRNA Vector clones and Carb resistant Reporter Vector clones for testing later this week.  </p>
   <p> Purified plasmid DNA from gRNA Vector clones and Carb resistant Reporter Vector clones for testing later this week.  </p>
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  <p> Test DNA! </P>
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<LI> Nanodrop to measure DNA concentration.
<LI> Nanodrop to measure DNA concentration.
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        <LI> Made P1 Buffer.
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<img class="pic" src="https://static.igem.org/mediawiki/2014/thumb/7/70/UTDallasAugust25i.jpg/800px-UTDallasAugust25i.jpg" width="300px" align="right"/>
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Today's Task
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       <img src="http://i.imgur.com/6gZs3aJ.jpg" height="400px">
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       <img src="https://static.igem.org/mediawiki/2014/thumb/d/dd/UTDallasAugust24.jpg/800px-UTDallasAugust24.jpg" height="400px">
       <br> Lots of Miniprep!
       <br> Lots of Miniprep!
       <div class="gallerylayer" opacity: 1; background: white;">
       <div class="gallerylayer" opacity: 1; background: white;">

Latest revision as of 14:33, 17 October 2014

Home Team Official Profile Project Parts Modeling Notebook Safety Attributions Human Practices

Sunday, August 24th, 2014

Click here to edit the page


Purified plasmid DNA from gRNA Vector clones and Carb resistant Reporter Vector clones for testing later this week.

Today's tasks:

  • Made glycerol stock and Miniprepped clones.
    • gRNA Vector clones: acfA (2), acfB (2), tcpF (2)
    • Carb Reporter Vector clones: tcpB (2), tcpQ (2), tcpR (1), tcpT (2)
    • No clones grew for acfB Carb Reporter Vector
  • Nanodrop to measure DNA concentration.
  • Made P1 Buffer.
Today's Task



Lots of Miniprep!