Team:NCTU Formosa/Notebook/test2

From 2014.igem.org

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<div class="calendar">
<div class="calendar">
<ul class="calendar-week">  
<ul class="calendar-week">  
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<li>Sunday</li>
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<li>Sun.</li>
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<li>Monday</li>
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<li>Mon.</li>
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<li>Tuesday</li>
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<li>Tue.</li>
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<li>Wednesday</li>
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<li>Wed.</li>
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<li>Thursday</li>
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<li>Thu.</li>
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<li>Friday</li>
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<li>Fri.</li>
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<li>Saturday</li>
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<li>Sat.</li>
</ul>
</ul>
<ul class="calendar-day">
<ul class="calendar-day">
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<li>23</li>
<li>23</li>
<li>24</li>
<li>24</li>
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</ul>
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<ul class="calendar-day">
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<li>25</li>
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<li>26</li>
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<li>27</li>
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<li>28</li>
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<li>29</li>
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<li>30</li>
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<li>31</li>
</ul>
</ul>
</div>
</div>
<div class="calendar-note">
<div class="calendar-note">
-
<div class="single note"><p>
+
<div class="single note">
-
5/1
+
<p>
-
Cultivation of [PSB1C3]Cr, [BFP]Cr, [T7promoter]ArKr, [T7 polymerase]Cr and [B0015]Ar (G2)
+
<font color="#FF0000"><span style="font-size:1cm;">5/1</span></font><br>
-
Transform of [T7 polymerase]Cr (G2)</p>
+
</p>
 +
<p><font color="yellow">Cultivation of</font> [PSB1C3]Cr, [BFP]Cr, [T7promoter]ArKr, [T7 polymerase]Cr and [B0015]Ar (G2)</p>
 +
<p><font color="yellow">Transformation of</font> [T7 polymerase]Cr (G2)</p>
-
<p>5/2
+
<p><font color="#FF0000"><span style="font-size:1cm;">5/2</span></font><br></p>
-
Cultivation of [J23101] Ar,[K592100] Cr,[B0032] Ar,[B0015] Ar (G1)
+
<p><font color="yellow">Cultivation of</font> [J23101] Ar,[K592100] Cr,[B0032] Ar,[B0015] Ar (G1)</p>
-
Mini-prep of [PSB1C3]Cr, [BFP]Cr, [T7promoter]ArKr, [T7 polymerase]Cr and [B0015]Ar (G2)
+
<p><font color="yellow">Mini-prep of</font> [PSB1C3]Cr, [BFP]Cr, [T7promoter]ArKr, [T7 polymerase]Cr and [B0015]Ar (G2)</p>
-
Digestion of [PSB1C3]Cr(EP), [BFP]Cr(XP), [T7 polymerase]Cr(ES), [T7promoter]ArKr(ES) and [B0015]Ar*2(ES & XP) (G2)</p>
+
<p><font color="yellow">Digestion of</font> [PSB1C3]Cr(EP), [BFP]Cr(XP), [T7 polymerase]Cr(ES), [T7promoter]ArKr(ES) and [B0015]Ar*2(ES & XP) (G2)</p>
 +
 
 +
<p><font color="#FF0000"><span style="font-size:1cm;">5/3</span></font><br></p>
 +
<p><font color="yellow">Mini-prep of</font> [J23101] Ar,[K592100] Cr,[B0032] Ar,[B0015] Ar (G1)</p>
 +
<p><font color="yellow">Mini-pep of</font> [B0032] Ar, [B0015] Ar,[BFP] Cr (G3)</p>
 +
<p><font color="yellow">Digestion of</font> [J23101] Ar (ES),[K592100]Cr(ES),[B0032] Ar(XP),[B0015] Ar(XP),[PSB1A3] Ar(EP),[PSB1C3] Cr(EP),[PSB1K3] Kr(EP) mini (G1)</p>
 +
<p><font color="yellow">Electrophoresis of</font> plasmid and digested product of [PSB1C3]Cr(EP), [BFP]Cr(XP),  [T7 polymerase]Cr(ES), [T7promoter]ArKr(ES) and  B0015*2(ES & XP) (G2)</p>
 +
<p><font color="yellow">Electrophoresis of</font> digested product of [J23101] Ar (ES),[K592100] Cr(ES),[B0032] Ar(XP),[B0015] Ar(XP),[PSB1A3] Ar(EP),[PSB1C3]Cr(EP),[PSB1K3] Kr(EP) (G1)</p>
 +
<p><font color="yellow">Cultivation of</font> [B0015] Ar, [PSB1A3] Ar, [PSB1K3] Kr (G1)</p>
 +
<p><font color="yellow">Ligation of</font> [BFP+B0032+PSB1K3]Kr (G2)</p>
 +
<p><font color="yellow">Ligation of</font> [J23101+B0032+PSB1C3] Cr (G1)</p>
 +
<p><font color="yellow">Transformation of</font> ligation product [BFP+B0032+PSB1K3]Kr (G2)</p>
 +
<p><font color="yellow">Transformation of</font> [T7polymerase]Cr and [B0015]Ar (G2)</p></div>
-
<p>5/3
 
-
Mini-prep of [J23101] Ar,[K592100] Cr,[B0032] Ar,[B0015] Ar (G1)
 
-
Mini-pep of [B0032] Ar, [B0015] Ar,[BFP] Cr (G3)
 
-
Digestion of [J23101] Ar (ES),[K592100]Cr(ES),[B0032] Ar(XP),[B0015] Ar(XP),[PSB1A3] Ar(EP),[PSB1C3] Cr(EP),[PSB1K3] Kr(EP) mini (G1)
 
-
Electrophoresis of plasmid and digested product of [PSB1C3]Cr(EP), [BFP]Cr(XP),  [T7 polymerase]Cr(ES), [T7promoter]ArKr(ES) and  B0015*2(ES & XP) (G2)
 
-
Electrophoresis of digested product of [J23101] Ar (ES),[K592100] Cr(ES),[B0032] Ar(XP),[B0015] Ar(XP),[PSB1A3] Ar(EP),[PSB1C3]Cr(EP),[PSB1K3] Kr(EP) (G1)
 
-
Cultivation of [B0015] Ar, [PSB1A3] Ar, [PSB1K3] Kr (G1)
 
-
Ligation: [BFP+B0032+PSB1K3]Kr (G2)
 
-
Ligation of [J23101+B0032+PSB1C3] Cr (G1)
 
-
Transform of ligation product [BFP+B0032+PSB1K3]Kr (G2)
 
-
Transform of [T7polymerase]Cr and [B0015]Ar (G2)</p><br></div>
 
<div  class="single note">
<div  class="single note">
 +
 +
 +
<p><font color="#FF0000"><span style="font-size:1cm;">5/4</span></font><br></p>
 +
<p><font color="yellow">Mini-prep of</font> [B0015] Ar, [PSB1A3] Ar, [PSB1K3] Kr (G1)</p>
 +
<p><font color="yellow">Mini-prep of</font> [B0015*3] Ar and [T7polymerase] Cr (G2)</p>
 +
<p><font color="yellow">Digestion of</font> [B0015] Ar(XP), [PSB1A3] Ar(EP), [PSB1K3] Kr (EP)(G1)</p>
 +
<p><font color="yellow">Digestion of</font> [B0015*3] Ar and [T7polymerase] Cr (G2)</p>
 +
<p><font color="yellow">Digestion of</font> [B0015] Ar(XP), [BFP] Cr(XP), [PSB1A3] Ar(EP), [PSB1C3] Cr(EP), [B0032] Ar (XP) (G3)</p>
 +
<p><font color="yellow">Transformation of</font> [P<sub>cons</sub> promoter] Ar, [RBS+BFP] Kr and [T7 promoter] Kr (G2)</p>
 +
<p><font color="yellow">Transformation of</font> [LacI] Ar (G3)</p>
 +
<p><font color="yellow">Electrophoresis of</font> plasmid and digested product of [B0015*3] Ar and [T7polymerase]Cr (G2)</p>
 +
<p><font color="yellow">Electrophoresis of</font> digested product of [B0015] Ar(XP), [PSB1A3] Ar(EP), [PSB1K3] Kr (EP), [K592100] Cr (ES)(G1)</p>
 +
<p><font color="yellow">Cultivation of</font> [B0015] Ar, [PSB1A3] Ar(G1)</p>
 +
<p><font color="yellow">Cultivation of</font> [Plac] Ar, [PSB1C3] Cr, [mGFP] Ar  (G3)</p>
 +
 +
<p><font color="#FF0000"><span style="font-size:1cm;">5/5</span></font><br></p>
 +
<p><font color="yellow">Mini-prep of</font> [PSB1A3] Ar,[B0015]Ar (G1)</p>
 +
<p><font color="yellow">Mini-prep of</font> [Plac] Ar, [PSB1C3] Cr, [mGFP] Ar (G3)</p>
 +
<p><font color="yellow">Digestion of</font> [PSB1A3] Ar(EP),[B0015]Ar(XP)(G1)</p>
 +
<p><font color="yellow">Electrophoresis of</font> digested product of [PSB1A3] Ar(EP),[B0015]Ar(XP),[PSB1K3]Kr(EP) (G1)</p>
 +
 +
<p>
 +
<font color="#FF0000"><span style="font-size:1cm;">5/6</span></font><br>
 +
</p>
 +
<p><font color="yellow">Mini-prep of</font> [LacI] Ar (G3)
 +
</p>
 +
<p>
 +
<font color="#FF0000"><span style="font-size:1cm;">5/7</span></font><br><p>
 +
<p><font color="yellow">Transformation of</font> [PT7polymerase]Cr and [J61048]Ar (G2)
 +
</p>
 +
<p>
 +
<font color="#FF0000"><span style="font-size:1cm;">5/8</span></font><br></p>
 +
<p>
 +
<font color="yellow">Transformation of</font> [B0015*3] Ar and [J61048*2] Ar (G2)</p>
 +
<p><font color="yellow">Transformation of</font> [B0015] Ar and [J61048] Ar (G3)</p>
 +
<p><font color="yellow">Transformation of</font> [B0015]Ar,[J61048]Ar,[J23101]Ar (G1)</p>
 +
<p><font color="yellow">Mini-prep of</font>  [PT7polymerase]Cr, [T7polymerase*2]Cr and [B0015*2]Ar (G2)</p>
 +
<p><font color="yellow">Digestion of</font> [T7polymerase] Kr (EP) and [B0015] Ar (ES) (G2)</p>
 +
<p><font color="yellow">Digestion of</font> [Plac] Ar (ES), [mGFP] Ar (ES), [LacI] Ar (ES), [PSB1C3] Cr (EP) (G3)</p>
 +
<p><font color="yellow">Electrophoresis of</font> digested product of [Plac] Ar (ES), [mGFP] Ar (ES), [LacI] Ar (ES), [PSB1C3] Cr (EP) (G3)
 +
</p>
 +
<p>
 +
<font color="#FF0000"><span style="font-size:1cm;">5/9</span></font><br></p>
 +
<p><font color="yellow">Cultivation of</font> [B0015] Ar, [J61048] Ar  [LacI] Ar (G3)
 +
</p>
 +
<p>
 +
<font color="#FF0000"><span style="font-size:1cm;">5/10</span></font><br></p>
 +
<p><font color="yellow">Mini-prep of</font> [LacI] Ar (G3)
 +
</p>
<br></div>
<br></div>
<div class="single note">
<div class="single note">
 +
<p><font color="#FF0000"><span style="font-size:1cm;">5/11</span></font><br></p><p>
 +
<font color="yellow">Cultivation of</font> [J61048] Ar (G2) (ES)</p><p>
 +
<font color="yellow">Electrophoresis of</font> plasmid and digested product of [B0015]Ar(ES), [T7polymerase] Kr(only mini) and [T7promoter] Kr (EP)</p><p>
 +
<font color="yellow">Transformation of</font> [T7 promoter] Kr (G2)</p><p>
 +
</p>
 +
<p>
 +
<font color="#FF0000"><span style="font-size:1cm;">5/12</span></font><br></p>
 +
<p><font color="yellow">Digestion of</font> [T7polymerase] Kr (ES) and [J61048] Ar (ES)  (G2)</p><p>
 +
<font color="#FF0000"><span style="font-size:1cm;">5/13</span></font><br></p><p>
 +
<font color="yellow">Transformation of</font> [T7 promoter] Kr (G2)</p>
 +
<p>
 +
<font color="#FF0000"><span style="font-size:1cm;">5/15</span></font><br></p><p>
 +
<font color="yellow">Electrophoresis of</font> plasmid and digested product of [J61048]Ar (ES), [T7polymerase*3]Kr(ES & XP) and [PSBIK3]Kr(EP) (G2)</p><p>
 +
<font color="yellow">Cultivation of</font> [B0015]Ar, [J61048]Ar, [J23101]Ar, [PSB1K3]Kr (G1)</p><p>
 +
<font color="yellow">Mini-prep of</font> [PSB1K3]Kr (G1)</p><p>
 +
<font color="yellow">Digestion of</font> [PSB1K3]Kr (EP) (G1)</p>
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<br></div>
<br></div>
<div class="single note">
<div class="single note">
 +
<p>
 +
<font color="#FF0000"><span style="font-size:1cm;">5/19</span></font><br></p>
 +
<p><font color="yellow">Cultivation of</font> [J23101]Ar,[B0015]Ar,[J61048]Ar (G1)
 +
</p><p>
 +
<font color="#FF0000"><span style="font-size:1cm;">5/20</span></font><br></p><p>
 +
<font color="yellow">Transformation of</font>  [T7polymerase] Kr and [PSB1K3] Kr (G2)</p><p>
 +
<font color="yellow">Electrophoresis of</font> plasmid and digested product of [T7polymerase*6] Kr and [PSB1K3*4]Kr (G2)</p><p>
 +
<font color="yellow">Mini-prep of</font> [J23101]Ar, [B0015]Ar, [J61048]Ar (G1)</p><p>
 +
<font color="yellow">Digestion of</font> [J23101]Ar (ES), [B0015]Ar (XP), [J61048]Ar (XP) (G1)</p><p>
 +
</p>
 +
<p>
 +
<font color="#FF0000"><span style="font-size:1cm;">5/21</span></font><br></p>
 +
<p>
 +
<font color="yellow">Digestion of</font> [T7polymerase*6] Kr and [PSB1K3*2] Kr (G2)</p><p>
 +
<font color="yellow">Mini-prep of</font> [T7polymerase] Kr and [PSB1K3] Kr (G2)</p><p>
 +
<font color="yellow">Electrophoresis of</font> plasmid and digested product of [J23101]Ar(ES), [B0015]Ar(XP), [J61048]Ar(XP) and digested product [PSB1K3]Kr(EP) (G1)</p><p>
 +
<font color="yellow">Ligation of</font> [K592100+J61048+PSB1K3] Kr (G1)</p><p>
 +
</p>
 +
<p>
 +
<font color="#FF0000"><span style="font-size:1cm;">5/22</span></font><br>
 +
</p>
 +
<p><font color="yellow">Electrophoresis of</font> plasmid and digested product of [T7polymerase] Kr (ES) and [PSB1K3]Kr (EP) (G2)
 +
</p>
 +
<p>
 +
<font color="#FF0000"><span style="font-size:1cm;">5/23</span></font><br>
 +
</p>
 +
<p>
 +
<font color="yellow">Transformation of</font> ligation product [K592100+J61048+PSB1K3]Kr (G1)</p><p>
 +
</p>
 +
<p>
 +
<font color="#FF0000"><span style="font-size:1cm;">5/24</span></font><br>
 +
</p>
 +
<p>
 +
<font color="yellow">Digestion of</font> [J61048] Ar (XP) (G2)</p><p>
 +
<font color="yellow">PCR of</font> [BFP+B0032*4] Kr and [T7 promoter*1] Ar Kr (G2)</p><p>
 +
<font color="yellow">Electrophoresis of</font> PCR products [BFP+B0032*4] Kr and [T7 promoter*1] Ar Kr, [J61048] Ar  (G2)</p><p>
 +
</p>
 +
<br></div>
 +
<div class="single note">
 +
<p>
 +
<font color="#FF0000"><span style="font-size:1cm;">5/27</span></font><br>
 +
</p>
 +
<p>
 +
<font color="yellow">Electrophoresis of</font> digested product of [mGFP] Ar (ES), [J61048] Ar (XP) ,[B0015] Ar (XP) (G3)
 +
</p>
 +
<p>
 +
<font color="yellow">Digestion of</font> [J61048] Ar (XP) and [T7polymerase] Kr (ES) (G2)
 +
</p>
 +
<p>
 +
<font color="yellow">Cultivation of</font> [PSB1K3*3] Kr(G2)
 +
</p>
 +
<p>
 +
<font color="#FF0000"><span style="font-size:1cm;">5/28</span></font><br>
 +
</p>
 +
<p>
 +
<font color="yellow">PCR of</font> [PSBIK3*5]Kr , [PSBIA3*5]Ar , [PSBIC3*5]Cr ,[J61048*5]Ar ,[B0015*5]Ar and [BFP*5](G1)
 +
</p>
 +
<p>
 +
<font color="yellow">Mini-prep of</font> [PSB1K3*3] Kr(G2)
 +
</p>
 +
<p>
 +
<font color="yellow">Digestion of</font>  [PSB1K3*3] Kr (EP) (G2)
 +
</p>
 +
<p>
 +
<font color="yellow">Electrophoresis of</font> plasmid and digested product of [PSB1K3] Kr (EP), [T7polymerase] Kr (ES), [J61048] Ar (XP) (G2)
 +
</p>
 +
<p>
 +
<font color="#FF0000"><span style="font-size:1cm;">5/29</span></font><br>
 +
</p>
 +
<p>
 +
<font color="yellow">Digestion of </font> [mGFP] Ar (ES), [J61048] Ar (XP) ,[B0015] Ar (XP) (G3)
 +
</p>
 +
<p>
 +
<font color="yellow">Transformation of </font> [J23101] Ar (G1)
 +
</p>
 +
<p>
 +
<font color="yellow">Transformation of </font> [T7 promoter] Ar Kr (G2)
 +
</p>
 +
<p>
 +
<font color="yellow">Electrophoresis of</font> plasmid and digested product of [J61048] Ar (ES), [T7polymerase] Kr (ES) (G2)
 +
</p>
 +
<p>
 +
<font color="yellow">Ligation of</font> [J61048+J61048] Cr, [T7polymerase+J61048] Kr, [RBS+BFP] Kr (G2)
 +
</p>
 +
<p>
 +
<font color="yellow">Ligation of </font> [Plac+B0032+PSB1K3]  Kr (G3)
 +
</p>
 +
<p>
 +
<font color="#FF0000"><span style="font-size:1cm;">5/30</span></font><br>
 +
</p>
 +
<p>
 +
<font color="yellow">Transformation of</font> ligation product (G2)
 +
</p>
 +
<p>
 +
<font color="yellow">Transformation of</font> ligation product [Plac+B0032+PSB1K3]  Kr (G3)
 +
</p>
<br></div>
<br></div>
</div>
</div>
<div class="clear"></div>
<div class="clear"></div>
</div>
</div>

Latest revision as of 14:21, 17 October 2014

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5/1

Cultivation of [PSB1C3]Cr, [BFP]Cr, [T7promoter]ArKr, [T7 polymerase]Cr and [B0015]Ar (G2)

Transformation of [T7 polymerase]Cr (G2)

5/2

Cultivation of [J23101] Ar,[K592100] Cr,[B0032] Ar,[B0015] Ar (G1)

Mini-prep of [PSB1C3]Cr, [BFP]Cr, [T7promoter]ArKr, [T7 polymerase]Cr and [B0015]Ar (G2)

Digestion of [PSB1C3]Cr(EP), [BFP]Cr(XP), [T7 polymerase]Cr(ES), [T7promoter]ArKr(ES) and [B0015]Ar*2(ES & XP) (G2)

5/3

Mini-prep of [J23101] Ar,[K592100] Cr,[B0032] Ar,[B0015] Ar (G1)

Mini-pep of [B0032] Ar, [B0015] Ar,[BFP] Cr (G3)

Digestion of [J23101] Ar (ES),[K592100]Cr(ES),[B0032] Ar(XP),[B0015] Ar(XP),[PSB1A3] Ar(EP),[PSB1C3] Cr(EP),[PSB1K3] Kr(EP) mini (G1)

Electrophoresis of plasmid and digested product of [PSB1C3]Cr(EP), [BFP]Cr(XP), [T7 polymerase]Cr(ES), [T7promoter]ArKr(ES) and B0015*2(ES & XP) (G2)

Electrophoresis of digested product of [J23101] Ar (ES),[K592100] Cr(ES),[B0032] Ar(XP),[B0015] Ar(XP),[PSB1A3] Ar(EP),[PSB1C3]Cr(EP),[PSB1K3] Kr(EP) (G1)

Cultivation of [B0015] Ar, [PSB1A3] Ar, [PSB1K3] Kr (G1)

Ligation of [BFP+B0032+PSB1K3]Kr (G2)

Ligation of [J23101+B0032+PSB1C3] Cr (G1)

Transformation of ligation product [BFP+B0032+PSB1K3]Kr (G2)

Transformation of [T7polymerase]Cr and [B0015]Ar (G2)

5/4

Mini-prep of [B0015] Ar, [PSB1A3] Ar, [PSB1K3] Kr (G1)

Mini-prep of [B0015*3] Ar and [T7polymerase] Cr (G2)

Digestion of [B0015] Ar(XP), [PSB1A3] Ar(EP), [PSB1K3] Kr (EP)(G1)

Digestion of [B0015*3] Ar and [T7polymerase] Cr (G2)

Digestion of [B0015] Ar(XP), [BFP] Cr(XP), [PSB1A3] Ar(EP), [PSB1C3] Cr(EP), [B0032] Ar (XP) (G3)

Transformation of [Pcons promoter] Ar, [RBS+BFP] Kr and [T7 promoter] Kr (G2)

Transformation of [LacI] Ar (G3)

Electrophoresis of plasmid and digested product of [B0015*3] Ar and [T7polymerase]Cr (G2)

Electrophoresis of digested product of [B0015] Ar(XP), [PSB1A3] Ar(EP), [PSB1K3] Kr (EP), [K592100] Cr (ES)(G1)

Cultivation of [B0015] Ar, [PSB1A3] Ar(G1)

Cultivation of [Plac] Ar, [PSB1C3] Cr, [mGFP] Ar (G3)

5/5

Mini-prep of [PSB1A3] Ar,[B0015]Ar (G1)

Mini-prep of [Plac] Ar, [PSB1C3] Cr, [mGFP] Ar (G3)

Digestion of [PSB1A3] Ar(EP),[B0015]Ar(XP)(G1)

Electrophoresis of digested product of [PSB1A3] Ar(EP),[B0015]Ar(XP),[PSB1K3]Kr(EP) (G1)

5/6

Mini-prep of [LacI] Ar (G3)

5/7

Transformation of [PT7polymerase]Cr and [J61048]Ar (G2)

5/8

Transformation of [B0015*3] Ar and [J61048*2] Ar (G2)

Transformation of [B0015] Ar and [J61048] Ar (G3)

Transformation of [B0015]Ar,[J61048]Ar,[J23101]Ar (G1)

Mini-prep of [PT7polymerase]Cr, [T7polymerase*2]Cr and [B0015*2]Ar (G2)

Digestion of [T7polymerase] Kr (EP) and [B0015] Ar (ES) (G2)

Digestion of [Plac] Ar (ES), [mGFP] Ar (ES), [LacI] Ar (ES), [PSB1C3] Cr (EP) (G3)

Electrophoresis of digested product of [Plac] Ar (ES), [mGFP] Ar (ES), [LacI] Ar (ES), [PSB1C3] Cr (EP) (G3)

5/9

Cultivation of [B0015] Ar, [J61048] Ar [LacI] Ar (G3)

5/10

Mini-prep of [LacI] Ar (G3)


5/11

Cultivation of [J61048] Ar (G2) (ES)

Electrophoresis of plasmid and digested product of [B0015]Ar(ES), [T7polymerase] Kr(only mini) and [T7promoter] Kr (EP)

Transformation of [T7 promoter] Kr (G2)

5/12

Digestion of [T7polymerase] Kr (ES) and [J61048] Ar (ES) (G2)

5/13

Transformation of [T7 promoter] Kr (G2)

5/15

Electrophoresis of plasmid and digested product of [J61048]Ar (ES), [T7polymerase*3]Kr(ES & XP) and [PSBIK3]Kr(EP) (G2)

Cultivation of [B0015]Ar, [J61048]Ar, [J23101]Ar, [PSB1K3]Kr (G1)

Mini-prep of [PSB1K3]Kr (G1)

Digestion of [PSB1K3]Kr (EP) (G1)


5/19

Cultivation of [J23101]Ar,[B0015]Ar,[J61048]Ar (G1)

5/20

Transformation of [T7polymerase] Kr and [PSB1K3] Kr (G2)

Electrophoresis of plasmid and digested product of [T7polymerase*6] Kr and [PSB1K3*4]Kr (G2)

Mini-prep of [J23101]Ar, [B0015]Ar, [J61048]Ar (G1)

Digestion of [J23101]Ar (ES), [B0015]Ar (XP), [J61048]Ar (XP) (G1)

5/21

Digestion of [T7polymerase*6] Kr and [PSB1K3*2] Kr (G2)

Mini-prep of [T7polymerase] Kr and [PSB1K3] Kr (G2)

Electrophoresis of plasmid and digested product of [J23101]Ar(ES), [B0015]Ar(XP), [J61048]Ar(XP) and digested product [PSB1K3]Kr(EP) (G1)

Ligation of [K592100+J61048+PSB1K3] Kr (G1)

5/22

Electrophoresis of plasmid and digested product of [T7polymerase] Kr (ES) and [PSB1K3]Kr (EP) (G2)

5/23

Transformation of ligation product [K592100+J61048+PSB1K3]Kr (G1)

5/24

Digestion of [J61048] Ar (XP) (G2)

PCR of [BFP+B0032*4] Kr and [T7 promoter*1] Ar Kr (G2)

Electrophoresis of PCR products [BFP+B0032*4] Kr and [T7 promoter*1] Ar Kr, [J61048] Ar (G2)


5/27

Electrophoresis of digested product of [mGFP] Ar (ES), [J61048] Ar (XP) ,[B0015] Ar (XP) (G3)

Digestion of [J61048] Ar (XP) and [T7polymerase] Kr (ES) (G2)

Cultivation of [PSB1K3*3] Kr(G2)

5/28

PCR of [PSBIK3*5]Kr , [PSBIA3*5]Ar , [PSBIC3*5]Cr ,[J61048*5]Ar ,[B0015*5]Ar and [BFP*5](G1)

Mini-prep of [PSB1K3*3] Kr(G2)

Digestion of [PSB1K3*3] Kr (EP) (G2)

Electrophoresis of plasmid and digested product of [PSB1K3] Kr (EP), [T7polymerase] Kr (ES), [J61048] Ar (XP) (G2)

5/29

Digestion of [mGFP] Ar (ES), [J61048] Ar (XP) ,[B0015] Ar (XP) (G3)

Transformation of [J23101] Ar (G1)

Transformation of [T7 promoter] Ar Kr (G2)

Electrophoresis of plasmid and digested product of [J61048] Ar (ES), [T7polymerase] Kr (ES) (G2)

Ligation of [J61048+J61048] Cr, [T7polymerase+J61048] Kr, [RBS+BFP] Kr (G2)

Ligation of [Plac+B0032+PSB1K3] Kr (G3)

5/30

Transformation of ligation product (G2)

Transformation of ligation product [Plac+B0032+PSB1K3] Kr (G3)


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