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Team:Paris Saclay/Notebook/July/9
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| + | =Wednesday 9th July= | ||
| + | ==Lab work== | ||
| + | ====Plasmid DNA Extraction==== | ||
| + | ''by Romain and Sean'' | ||
| - | + | Plasmids used: DH5αZ1 pSB1C3 RBS amilCP Ter clone 9, A and B. | |
| - | + | [https://2014.igem.org/Team:Paris_Saclay/Protocols/Extraction_of_the_Genomic_DNA_from_Bacteria_by_using_NucleoSpin®_Tissue Protocol] | |
| - | '' | + | ====Electrophoresis==== |
| + | ''by Arnaud, Romain and Sean'' | ||
| - | Plasmids used: DH5αZ1 | + | Plasmids used: |
| + | *DH5αZ1 pSB1C3 RBS amilCP Ter clone 9, A. | ||
| + | *DH5αZ1 pSB1C3 RBS amilCP Ter clone 9, B. | ||
| + | *XL1-Blue pCola-ges Kan | ||
| + | *pJBEI-6409 Cm | ||
| - | [https:// | + | [https://2014.igem.org/Team:Paris_Saclay/Protocols/Gel_Electrophoresis Protocol] |
| - | ==== | + | ====New culture==== |
| - | + | ''by Romain'' | |
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| - | '' | + | |
New liquid cultures of: | New liquid cultures of: | ||
*FNR-RBS-amilCp-Ter DH5αZ1 P5B1C3 Cm | *FNR-RBS-amilCp-Ter DH5αZ1 P5B1C3 Cm | ||
| - | *BBa B0015 dble ter | + | *BBa B0015 dble ter pSB1C3 Cm |
| - | We incubate cultures at 37°C. | + | 4x 5ml LB + 20μl Chloramphenicol + a little of bacteria. We incubate cultures at 37°C. |
| - | == | + | ==Reunion== |
Conference with the team to discuss about the project. The handled topics were: | Conference with the team to discuss about the project. The handled topics were: | ||
* Definition of Biobricks to make | * Definition of Biobricks to make | ||
| Line 41: | Line 37: | ||
* Sponsoring | * Sponsoring | ||
* Wiki's progress | * Wiki's progress | ||
| - | * | + | * Modeling's progress |
| + | ==Photo of the Day== | ||
| + | [[File:Paris Saclay 9_july.jpg|600px|center]] | ||
| - | + | '''Members present:''' | |
* Instructors and advisors: Alice, Olivier, Solenne and Sylvie. | * Instructors and advisors: Alice, Olivier, Solenne and Sylvie. | ||
* Students: Arnaud, Fabio, Juliette, Maher, Marie, Mathias, Mathieu, Pierre, Romain and Sean. | * Students: Arnaud, Fabio, Juliette, Maher, Marie, Mathias, Mathieu, Pierre, Romain and Sean. | ||
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| + | {{Team:Paris_Saclay/notebook_footer}} | ||
Latest revision as of 13:57, 17 October 2014
Contents |
Wednesday 9th July
Lab work
Plasmid DNA Extraction
by Romain and Sean
Plasmids used: DH5αZ1 pSB1C3 RBS amilCP Ter clone 9, A and B.
Electrophoresis
by Arnaud, Romain and Sean
Plasmids used:
- DH5αZ1 pSB1C3 RBS amilCP Ter clone 9, A.
- DH5αZ1 pSB1C3 RBS amilCP Ter clone 9, B.
- XL1-Blue pCola-ges Kan
- pJBEI-6409 Cm
New culture
by Romain
New liquid cultures of:
- FNR-RBS-amilCp-Ter DH5αZ1 P5B1C3 Cm
- BBa B0015 dble ter pSB1C3 Cm
4x 5ml LB + 20μl Chloramphenicol + a little of bacteria. We incubate cultures at 37°C.
Reunion
Conference with the team to discuss about the project. The handled topics were:
- Definition of Biobricks to make
- Planning of internships
- Definition of Biobricks to render
- Understanding the construction of consecutive genes
- Organisation and planning of experiences to do
- Sponsoring
- Wiki's progress
- Modeling's progress
Photo of the Day
Members present:
- Instructors and advisors: Alice, Olivier, Solenne and Sylvie.
- Students: Arnaud, Fabio, Juliette, Maher, Marie, Mathias, Mathieu, Pierre, Romain and Sean.
