Team:TCU Taiwan/Project

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     <ul>
     <ul>
       <li><a href="https://2014.igem.org/Team:TCU_Taiwan/Team"><font size="3">Members</font></a></li>
       <li><a href="https://2014.igem.org/Team:TCU_Taiwan/Team"><font size="3">Members</font></a></li>
 +
      <li><a href="https://2014.igem.org/Team:TCU_Taiwan/Achievements"><font size="3">Achievements</font></a></li>
       <li><a href="https://igem.org/Team.cgi?id=1473" target="_blank"><font size="3">Official Team Profile</font></a></li>
       <li><a href="https://igem.org/Team.cgi?id=1473" target="_blank"><font size="3">Official Team Profile</font></a></li>
       <li><a href="https://2014.igem.org/Team:TCU_Taiwan/Contact"><font size="3">Contact</font></a></li>
       <li><a href="https://2014.igem.org/Team:TCU_Taiwan/Contact"><font size="3">Contact</font></a></li>
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onMouseOver="this.bgColor='#99CC69'" onMouseOut="this.bgColor='#FFCE5B'" bgColor=#FFCE5B>   
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style="color:#0B6D78; text-decoration: none;"><font face="Trebuchet MS" size="5">Topic 1</font></a> </td>
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style="color:#0B6D78; text-decoration: none;"><font face="Trebuchet MS" size="5">Protagonist</font></a> </td>
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style="color:#0B6D78; text-decoration: none;"><font face="Trebuchet MS" size="5">Super Cast</font></a> </td>
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<td style="border:0px solid black;" align="center"  width="33%" height ="35px"  
<td style="border:0px solid black;" align="center"  width="33%" height ="35px"  
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style="color:#0B6D78; text-decoration: none;"><font face="Trebuchet MS" size="5">Topic 3</font></a> </td>
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style="color:#0B6D78; text-decoration: none;"><font face="Trebuchet MS" size="5">The Story</font></a> </td>
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<td style="border:0px solid black;" align="center"  width="33%" height ="35px"  
<td style="border:0px solid black;" align="center"  width="33%" height ="35px"  
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<a href="#Program"
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style="color:#0B6D78; text-decoration: none;"><font face="Trebuchet MS" size="5">References</font></a> </td>
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style="color:#0B6D78; text-decoration: none;"><font face="Trebuchet MS" size="5">Program</font></a> </td>
</tr>
</tr>
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<tr>
<tr>
   <td align="right" height ="35px"><img src="https://static.igem.org/mediawiki/2014/b/b8/TCU-Phage.png" width="30%"/></td>
   <td align="right" height ="35px"><img src="https://static.igem.org/mediawiki/2014/b/b8/TCU-Phage.png" width="30%"/></td>
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<tr ><td coslpan="3" height="35px"></td></tr>
<tr ><td coslpan="3" height="35px"></td></tr>
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<tr><td colspan="3" style="background-color:#FFF2B5" height="20px"> <font face="Trebuchet MS" size="7" color="#A66B38" id="abstract">Abstract</font> </td> </tr>
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<tr><td colspan="3" style="background-color:#FFF2B5" height="20px"> <font face="Trebuchet MS" size="6" color="#A66B38" id="abstract">Abstract</font> </td> </tr>
<tr>
<tr>
   <td valign="top" height="10px">&nbsp;</td>
   <td valign="top" height="10px">&nbsp;</td>
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<table width="975px" align="center">
<table width="975px" align="center">
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<tr> <td> <h3 id="topic1">Topic 1</h3> </td> </tr>
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<tr> <td style="background-color:#FFF2B5" height="20px"> <h3 id="topic1"><font face="Trebuchet MS" size="6" color="#A66B38">Protagonist</font></h3> </td> </tr>
<tr>
<tr>
<!-- first column -->
<!-- first column -->
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<td width="30%">
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<td width="50%" colspan="3">
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<p>&nbsp;</p>
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<font size="4" face="Verdana" color="#333"><p>Trogene horse is a lovely wooden horse, but he carries a super  weapon inside, and this weapon can help greek soldiers defeat Troy city.</p></font>
</td>
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<td width="30"></td>
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<td align="center">
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<img src="https://static.igem.org/mediawiki/2014/b/b7/2014placeholderimage.jpg" width="300px" height="300px">
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<img src="https://static.igem.org/mediawiki/2014/c/c2/TCU-Logo-up.png" width="490" height="381">
</td>
</td>
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<table width="975px" align="center">
<table width="975px" align="center">
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<tr> <td> <h3 id="topic2">Topic 2</h3>  </td> </tr>
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<tr> <td style="background-color:#FFF2B5" height="20px"> <h3 id="topic2"><font face="Trebuchet MS" size="6" color="#A66B38" id="abstract">Super Cast</font></h3>  </td> </tr>
<tr>
<tr>
<!-- first column -->
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<td width="30%">
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<td align="center">
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<img src="https://static.igem.org/mediawiki/2014/b/b7/2014placeholderimage.jpg" width="300px" height="300px">
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<img src="https://static.igem.org/mediawiki/2014/c/cc/TCU-Anti.png" width="200px">
</td>
</td>
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<td width="5%"></td>
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<td width="10">&nbsp;</td>
 +
<td><font size="5" face="Verdana" color="#0092C7"><p>Antibiotics：</p></font><font size="4" face="Verdana" color="#333">
 +
  <p>A  group of greek soldiers who is eager to conquest troy city. </p>
 +
</font></td>
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<td width="30">&nbsp;</td>
<!-- second column -->
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<td width="30%">
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<td align="center">
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<img src="https://static.igem.org/mediawiki/2014/b/b7/2014placeholderimage.jpg" width="300px" height="300px">
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<img src="https://static.igem.org/mediawiki/2014/d/d3/TCU_Igem_cell.png" width="120">
</td>
</td>
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<td width="5%"></td>
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<td width="10">&nbsp;</td>
<!-- third column -->
<!-- third column -->
<td width="30%">
<td width="30%">
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<p>&nbsp;</p>
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<font size="5" face="Verdana" color="#0092C7"><p>Bacterium：</p></font><font size="4" face="Verdana" color="#333">
 +
<p>Troy  citizens who lives happily under the protection of troy wall. </p>
 +
</font>
</td>
</td>
</tr>
</tr>
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<table width="975px" align="center">
<table width="975px" align="center">
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<tr> <td> <h3 id="topic3">Topic 3</h3>  </td> </tr>
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<tr> <td> <h3 id="topic3">&nbsp;</h3>  </td> </tr>
<tr>
<tr>
<!-- first column -->
<!-- first column -->
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<td width="65%">
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<td align="center" width="30%">
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<p>&nbsp;</p>
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<p><img src="https://static.igem.org/mediawiki/2014/7/7e/TCU_WALL.png" width="300" height="300" /></p>
</td>
</td>
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<td width="5%"></td>
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<td width="30"></td>
<!-- second column -->
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<td width="30%">
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<td><font size="5" face="Verdana" color="#0092C7"><p>Troy Wall: </p></font><font size="4" face="Verdana" color="#333">
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<img src="https://static.igem.org/mediawiki/2014/b/b7/2014placeholderimage.jpg" width="300px" height="200px">
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  <p>A great wall of Troy city, it is the nightmare of  greek soldiers. </p>
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</td>
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</font></td>
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</tr>
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<tr>
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  <td align="center" height="30">&nbsp;</td>
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  <td></td>
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  <td>&nbsp;</td>
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</tr>
 +
<tr>
 +
  <td align="center"><img src="https://static.igem.org/mediawiki/2014/1/11/TCU_GODDESS.png" width="300" height="300" /></td>
 +
  <td></td>
 +
  <td><font size="5" face="Verdana" color="#0092C7"><p>TCU-Taiwan：</p></font><font size="4" face="Verdana" color="#333">
 +
    <p>Greek  soldiers&rsquo; goddess who designed secret agents can break troy wall and hide them  inside the Trogene Horse. With this, Trogene Horse become a powerful weapon of  greek soldiers.</p>
 +
  </font></td>
</tr>
</tr>
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</table>
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<table width="975" border="0" cellspacing="0" cellpadding="0" align="center">
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    <td height="30">&nbsp;</td>
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  <tr>
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    <td style="background-color:#FFF2B5" height="20px" align="center"><h3 id="Story"><font face="Trebuchet MS" size="6" color="#A66B38">The Story</font></h3> </td>
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  </tr>
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  <tr>
 +
    <td height="15">&nbsp;</td>
 +
  </tr>
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  <tr>
 +
    <td align="center">
 +
    <video width="640" height="360" controls>
 +
<source src="https://static.igem.org/mediawiki/2014/9/95/TCU-Taiwan_An_Introduction_of_Trogene_Horse_%28720p%29.mov" type="video/mp4">
 +
<iframe width="853" height="480" src="//www.youtube.com/embed/jMb2o3Bwiq8?showinfo=0" frameborder="0" allowfullscreen></iframe>
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<object>
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Your browser does not support the HTML5 video tag!
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<a href="https://static.igem.org/mediawiki/2014/9/95/TCU-Taiwan_An_Introduction_of_Trogene_Horse_%28720p%29.mov"><br>Download the video.</a>
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</object>
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</video>
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    </td>
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  </tr>
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</table>
<!-- references -->
<!-- references -->
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<tr ><td height="35px"></td></tr>
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<tr><td> <h3 id="references">References</h3>
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<table width="975" border="0" cellspacing="0" cellpadding="0" align="center">
   <tr>
   <tr>
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     <td  style="background-color:#FFF2B5" height="20px"><font face="Trebuchet MS" size="7" color="#A66B38" id="abstract">Program</font></td>
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     <td  style="background-color:#FFF2B5" height="20px" align="center"><font face="Trebuchet MS" size="6" color="#A66B38" id="Program">Program</font></td>
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         <td align="center" width="15">&nbsp;</td>
         <td align="center" width="15">&nbsp;</td>
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         <td align="center" width="250" height="250"><img src="https://static.igem.org/mediawiki/2014/e/e5/TCU-Part-logo.png" alt="Parts" width="128"onmouseover="this.width=this.width*1.2;Partsbox.color='#90B849';" onmouseout="this.width=this.width/1.2;Partsbox.color='#333';"/></td>
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         <td align="center" width="250" height="200"><a href="https://2014.igem.org/Team:TCU_Taiwan/Parts"><img src="https://static.igem.org/mediawiki/2014/e/e5/TCU-Part-logo.png" alt="Parts" width="128" onmouseover="this.width=this.width*1.2;Partsbox.color='#90B849';" onmouseout="this.width=this.width/1.2;Partsbox.color='#333';"/></a></td>
         <td width="20">&nbsp;</td>
         <td width="20">&nbsp;</td>
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         <td align="center" width="250"><img src="https://static.igem.org/mediawiki/2014/4/4d/TCU_Blueprint-128.png" width="128" height="128"  alt="System Design"/></td>
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         <td align="center" width="250"><a href="https://2014.igem.org/Team:TCU_Taiwan/SystemDesign"><img src="https://static.igem.org/mediawiki/2014/4/4d/TCU_Blueprint-128.png" width="128"alt="System Design" onmouseover="this.width=this.width*1.2;Systembox.color='#90B849';" onmouseout="this.width=this.width/1.2;Systembox.color='#333';"/></a></td>
         <td width="20">&nbsp;</td>
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         <td align="center" width="250"><img src="https://static.igem.org/mediawiki/2014/2/24/TCU_Microscope-128.png" alt="M13 Phage" width="128" height="128" /></td>
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         <td align="center" width="250"><a href="https://2014.igem.org/Team:TCU_Taiwan/M13Phage"><img src="https://static.igem.org/mediawiki/2014/2/24/TCU_Microscope-128.png" alt="M13 Phage" width="128" onmouseover="this.width=this.width*1.2;M13box.color='#90B849';" onmouseout="this.width=this.width/1.2;M13box.color='#333';" /></a></td>
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         <td align="center" width="250" height="250"><img src="https://static.igem.org/mediawiki/2014/4/43/TCU_Gear-128.png" alt="CRISPR" width="128" height="128" /></td>
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         <td align="center" width="250" height="200"><a href="https://2014.igem.org/Team:TCU_Taiwan/CRISPR"><img src="https://static.igem.org/mediawiki/2014/4/43/TCU_Gear-128.png" alt="CRISPR" width="128" onmouseover="this.width=this.width*1.2;CRISPRbox.color='#90B849';" onmouseout="this.width=this.width/1.2;CRISPRbox.color='#333';"/></a></td>
         <td>&nbsp;</td>
         <td>&nbsp;</td>
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         <td align="center"><img src="https://static.igem.org/mediawiki/2014/4/4a/TCU_1413400508_Briefcase.png" alt="Future Works" width="128" height="128" /></td>
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         <td align="center"><a href="https://2014.igem.org/Team:TCU_Taiwan/FutureWorks"><img src="https://static.igem.org/mediawiki/2014/4/4a/TCU_1413400508_Briefcase.png" alt="Future Works" width="128" onmouseover="this.width=this.width*1.2;Futurebox.color='#90B849';" onmouseout="this.width=this.width/1.2;Futurebox.color='#333';"/></a></td>
         <td>&nbsp;</td>
         <td>&nbsp;</td>
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         <td align="center"><img src="https://static.igem.org/mediawiki/2014/d/dd/TCU-Human-logo.png" alt="Human Pratics" width="128" height="128" /></td>
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         <td align="center"><a href="https://2014.igem.org/Team:TCU_Taiwan/HumanPratics"><img src="https://static.igem.org/mediawiki/2014/d/dd/TCU-Human-logo.png" alt="Human Pratics" width="128" onmouseover="this.width=this.width*1.2;Humanbox.color='#90B849';" onmouseout="this.width=this.width/1.2;Humanbox.color='#333';"/></a></td>
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  <td style="background-color:#FFF2B5" height="20px"><font face="Trebuchet MS" size="7" color="#A66B38">Trogene Horse</font></td>
 
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<font size="3" face="Verdana" color="#333"><p>To access this, we use a special <a href="http://en.wikipedia.org/wiki/Phagemid" target="_blank">phagemid</a>  for phage display. The phagemid contains a additional phage replication origin.  When helper phage (a phage whose own ori is knock-out) infection take place,  phagemid can be packaged as genome for progeny. Since we put an <a href="http://en.wikipedia.org/wiki/Antibiotic_resistance" target="_blank">antibiotic</a>  resistance gene targeting <a href="http://en.wikipedia.org/wiki/CRISPR" target="_blank">CRISPR</a> system on our phagemid, this phage display can  spread our CRISPR among bacterium nearby and remove their resistance  efficiently.</p></font>
 
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<font size="3" face="Verdana" color="#333"><p>Trogene Horse is a new concept combining  phage therapy with CRISPR, and this combination can solve both safety  concern(phage therapy) and efficiency problem(CRISPR). We believe our project  can deal with bacterial resistance problem on agriculture, and hope it can  access on medicine one day.</p></font></td>
 
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        <td align="center" width="250" height="250"><img src="https://static.igem.org/mediawiki/2014/e/e5/TCU-Part-logo.png" alt="Parts" width="128"onmouseover="this.width=this.width*1.2;Partsbox.color='#90B849'" onmouseout="this.width=this.width/1.2;Partsbox.color='#333'"/></td>
 
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        <td align="center" width="250"><img src="https://static.igem.org/mediawiki/2014/4/4d/TCU_Blueprint-128.png" width="128" height="128"  alt="System Design"/></td>
 
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        <td align="center" width="250"><img src="https://static.igem.org/mediawiki/2014/2/24/TCU_Microscope-128.png" alt="M13 Phage" width="128" height="128" /></td>
 
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        <td align="center" width="250" height="250"><img src="https://static.igem.org/mediawiki/2014/4/43/TCU_Gear-128.png" alt="CRISPR" width="128" height="128" /></td>
 
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        <td align="center"><img src="https://static.igem.org/mediawiki/2014/4/4a/TCU_1413400508_Briefcase.png" alt="Future Works" width="128" height="128" /></td>
 
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    <a href="https://2014.igem.org/Team:TCU_Taiwan/Contact" title="Contact Us"><img src="https://static.igem.org/mediawiki/2014/8/84/TCU-Contact-bot.png" width="80" onMouseMove="this.src=Image0.src;" onMouseOut="this.src='https://static.igem.org/mediawiki/2014/8/84/TCU-Contact-bot.png'"></a>
 
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    <a href="mailto: tcutaiwan@gmail.com" target="_blank" title="Gmail"><img src="https://static.igem.org/mediawiki/2014/c/c4/TCU%E3%84%A6Gmail.png" width="30"></a>&nbsp;
 
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    <a href="https://www.facebook.com/TCUiGEM?ref=ts&fref=ts"  target="_blank" title="Facebook"><img src="https://static.igem.org/mediawiki/2014/b/b9/TCU-Facebook.png" width="30"></a>&nbsp;<a href="https://www.youtube.com/channel/UCr40ZmaFNJGx7tUufCXK_OQ" target="_blank" title="YouTube"><img src="https://static.igem.org/mediawiki/2014/2/29/TCU_Youtube.png" width="30"></a>&nbsp;<a href="http://tcuigem.wordpress.com/" target="_blank" title="Wordpress"><img src="https://static.igem.org/mediawiki/2014/f/f0/TCU-Wordpress.png" width="30"></a>
 
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          <td><a href="https://2014.igem.org/Team:TCU_Taiwan/Team" title="Team Members"><img src="https://static.igem.org/mediawiki/2014/1/1a/TCU-Team-logo.png" width="100"onMouseOver="this.width=this.width*1.1;Members.color='#333'" onMouseOut="this.width=this.width/1.1;Members.color='#F2DE79'"></a></td>
 
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          <td><a href="https://2014.igem.org/Team:TCU_Taiwan/Project" title="Project"><img src="https://static.igem.org/mediawiki/2014/f/f4/TCU-Project-logo.png" width="100"  onMouseOver="this.width=this.width*1.1;Project.color='#333'" onMouseOut="this.width=this.width/1.1;Project.color='#F2DE79'"></a></td>
 

Latest revision as of 13:32, 17 October 2014


 
Project
  
   
Trogene Horse  
Abstract
Protagonist
Super Cast
The Story
Program
Abstract
   

To access this, we use a special phagemid for phage display. The phagemid contains a additional phage replication origin. When helper phage (a phage whose own ori is knock-out) infection take place, phagemid can be packaged as genome for progeny. Since we put an antibiotic resistance gene targeting CRISPR system on our phagemid, this phage display can spread our CRISPR among bacterium nearby and remove their resistance efficiently.

Trogene Horse is a new concept combining phage therapy with CRISPR, and this combination can solve both safety concern(phage therapy) and efficiency problem(CRISPR). We believe our project can deal with bacterial resistance problem on agriculture, and hope it can access on medicine one day.

Protagonist

Trogene horse is a lovely wooden horse, but he carries a super weapon inside, and this weapon can help greek soldiers defeat Troy city.

Super Cast
 

Antibiotics：

A group of greek soldiers who is eager to conquest troy city.

   

Bacterium：

Troy citizens who lives happily under the protection of troy wall.

 

Troy Wall:

A great wall of Troy city, it is the nightmare of greek soldiers.

   

TCU-Taiwan：

Greek soldiers’ goddess who designed secret agents can break troy wall and hide them inside the Trogene Horse. With this, Trogene Horse become a powerful weapon of greek soldiers.
 

The Story
 
Program
 
  Parts   System Design   M13 Phage  
             
  Parts   System Design   M13 Phage  
             
  CRISPR   Future Works   Human Pratics  
             
  CRISPR   Future Work   Human Pratics  
 
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Team Members Project Parts Human Pratics Modeling Safety Notebook Attributions

Lost the way? Use it to help you if you're lost.

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