Team:UT-Dallas/Notebook/8-19

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<h1 class="firstHeading" align="center">Wednesday, August 13, 2014</h1>
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<a href="https://2014.igem.org/Team:UT-Dallas"style="color:#ffffff; text-decoration: none; text-transform: uppercase; font-weight: bold;" >Home </a> </td>
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<a href="https://2014.igem.org/Team:UT-Dallas/Team"style="color:#ffffff; text-decoration: none; text-transform: uppercase; font-weight: bold;"> Team </a> </td>
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<a href="https://igem.org/Team.cgi?year=2014&team_name=UT-Dallas"style="color:#ffffff; text-decoration: none; text-transform: uppercase; font-weight: bold;"> Official Profile </a></td>
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<a href="https://2014.igem.org/Team:UT-Dallas/Project"style="color:#ffffff; text-decoration: none; text-transform: uppercase; font-weight: bold;"> Project</a></td>
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<a href="https://2014.igem.org/Team:UT-Dallas/Modeling"style="color:#ffffff; text-decoration: none; text-transform: uppercase; font-weight: bold;"> Modeling</a></td>
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<a href="https://2014.igem.org/Team:UT-Dallas/Notebook"style="color:#ffffff; text-decoration: none; text-transform: uppercase; font-weight: bold;"> Notebook</a></td>
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<a href="https://2014.igem.org/Team:UT-Dallas/Safety"style=" color:#ffffff; text-decoration: none; text-transform: uppercase; font-weight: bold;"> Safety </a></td>
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<a href="https://2014.igem.org/Team:UT-Dallas/Attributions"style="color:#ffffff; text-decoration: none; text-transform: uppercase; font-weight: bold;"> Attributions </a></td>
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<a href="https://2014.igem.org/Team:UT-Dallas/Human-Practices"style="color:#ffffff; text-decoration: none; text-transform: uppercase; font-weight: bold;"> Human Practices</a></td>
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<h1 class="firstHeading" align="center">Tuesday, August 19, 2014</h1>
<center><p><a href="https://2014.igem.org/wiki/index.php?title=Team:UT-Dallas/Notebook/8-19&action=edit "style="color:#87A96B" >Click here to edit the page </a> </p></center>
<center><p><a href="https://2014.igem.org/wiki/index.php?title=Team:UT-Dallas/Notebook/8-19&action=edit "style="color:#87A96B" >Click here to edit the page </a> </p></center>
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   Today is a short day. We are test digesting Tra's minipreped first batch of reporter vectors on Carb backbone in the weekend. Two clones failed so we picked 2 colonies from each failed clone plates and inoculate them. We will miniprep and test digesting them again tomorrow. We threw out the miniprep products and glycerol stocks for the failed one. We got some confusion with the gel because Tra loaded multiple wells with the same sample. (Sorry for the confusion! - Tra)</p>
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   Today is a short day. Yesterday, we picked 2 colonies from each failed clone plates and inoculate them; today, we minipreped and test digested them. They failed the test digest so we inoculate the rest of the colonies on the plates.</p>
<p class="tab">
<p class="tab">
We are sending the gRNA from the third batch and some other reporter vectors for sequencing.</p>
We are sending the gRNA from the third batch and some other reporter vectors for sequencing.</p>
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<p class="tab">
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We also digest RBS-Carb to get more Carb back bone for later use.</p>
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Yesterday, we digested more RBS-Carb; today, we ran it on gel and gel purified it. The concentration at the end was good.</p>
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We started the third batch last week with some gel digest testing of gRNA. Only one (tcpF) failed. We are building our reporter vectors today from PCR products.</p>
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Our clones on the plates transformed yesterday were good: many colonies on the plates and none on the negative.</p>
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<strike>We also had a lively discussion about bugs. Tra's new apartment has bed bugs. Ewww!</strike>
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<strike>Orientation! School is starting next week! (25/8) >.<</strike>
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<LI> Miniprep ctxA, ctxB.
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<LI> glycerol stock and Miniprep of ctxA, ctxB inoculated on 08/18/14.
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<LI> Test digest and ran gel for ctxA, ctxB.
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<LI> Test digest and ran gel for ctxA, ctxB and gel electrophoresis.
<LI> Inoculate 2 colonies from each plate transformed yesterday (second batch, reporter vectors on Carb).
<LI> Inoculate 2 colonies from each plate transformed yesterday (second batch, reporter vectors on Carb).
<LI> Prepared for sequencing: diluted to the correct volume and concentration.
<LI> Prepared for sequencing: diluted to the correct volume and concentration.
<LI> Purified RBS (digested overnight yesterday).
<LI> Purified RBS (digested overnight yesterday).
<LI> Transformed of overnight ligation yesterday (third batch, reporter vectors with promoters under Chlora).
<LI> Transformed of overnight ligation yesterday (third batch, reporter vectors with promoters under Chlora).
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<LI> Inoculate more colonies from ctxA, ctxB plates.
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Today's Task
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       <br> Rishika sitting on the table.  
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       <br> RBS-Carb Gel was good.  
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       <img src="https://static.igem.org/mediawiki/2014/thumb/b/bf/UTDallasAugust22i4.jpg/450px-UTDallasAugust22i4.jpg" style="border-width: 0px; display: inline; margin-left: 0px; margin-top: 0px;" height="400px">
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<br> Description of image from today
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      <br> Updating the wiki notebook from the slowest computer in the building because currently we can't log into any other computer. Rishika is gel-ing the ctx-s.
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Latest revision as of 13:00, 17 October 2014

Home Team Official Profile Project Parts Modeling Notebook Safety Attributions Human Practices

Tuesday, August 19, 2014

Click here to edit the page

Today is a short day. Yesterday, we picked 2 colonies from each failed clone plates and inoculate them; today, we minipreped and test digested them. They failed the test digest so we inoculate the rest of the colonies on the plates.

We are sending the gRNA from the third batch and some other reporter vectors for sequencing.

Yesterday, we digested more RBS-Carb; today, we ran it on gel and gel purified it. The concentration at the end was good.

Our clones on the plates transformed yesterday were good: many colonies on the plates and none on the negative.

Orientation! School is starting next week! (25/8) >.<

Today's tasks:
  • glycerol stock and Miniprep of ctxA, ctxB inoculated on 08/18/14.
  • Test digest and ran gel for ctxA, ctxB and gel electrophoresis.
  • Inoculate 2 colonies from each plate transformed yesterday (second batch, reporter vectors on Carb).
  • Prepared for sequencing: diluted to the correct volume and concentration.
  • Purified RBS (digested overnight yesterday).
  • Transformed of overnight ligation yesterday (third batch, reporter vectors with promoters under Chlora).
  • Inoculate more colonies from ctxA, ctxB plates.
Today's Task


RBS-Carb Gel was good.


Updating the wiki notebook from the slowest computer in the building because currently we can't log into any other computer. Rishika is gel-ing the ctx-s.

Retrieved from "http://2014.igem.org/Team:UT-Dallas/Notebook/8-19"