Team:UT-Dallas/Notebook/8-18

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Today is a long day. We are test digesting Tra's minipreped first batch of reporter vectors on Carb backbone in the weekend. We are digesting second batch of reporter vectors, ligate and transform them.
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Hopefully tomorrow there will be colonies on our plates.
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We also digest RBS-Carb to get more Carb back bone for later use.
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<a href="https://2014.igem.org/Team:UT-Dallas"style="color:#ffffff; text-decoration: none; text-transform: uppercase; font-weight: bold;" >Home </a> </td>
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We started the third batch last week with some gel digest testing of gRNA. Only one (tcpF) failed. We are building our reporter vectors today from PCR products.
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<h1 class="firstHeading" align="center">Monday, August 18, 2014</h1>
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<center><p><a href="https://2014.igem.org/wiki/index.php?title=Team:UT-Dallas/Notebook/8-18&action=edit "style="color:#87A96B" >Click here to edit the page </a> </p></center>
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  Today is a long day. We are test digesting Tra's minipreped first batch of reporter vectors on Carb backbone from this past weekend. Two clones failed so we picked 2 colonies from each failed clone plates ctxA(2) and ctxB(2) and inoculated them. We will miniprep and test digest them again tomorrow. We threw out the miniprep products and glycerol stocks for the failed one. We got some confusion with the gel because Tra loaded multiple wells with the same sample.</p>
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We are digesting second batch of reporter vectors, and performing a 30 minute ligate on Carb backbone and then transform them. Hopefully tomorrow there will be colonies on our plates. We will pick the colonies, inoculate. The day after tomorrow we will glycerol stock them, miniprep and test digest our product. Hopefully we will have good product to complete parts of our BioBricks, (and we won't have to redo everything again) and we will keep them for later experiments.</p>
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We also digested RBS-Carb to get more Carb back bone for later use.</p>
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Lastly we did an overnight ligation for the third batch of reporters and promotors.</p>
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<LI> Inoculate more colonies (2) ctxA and (2) ctxB.
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<LI> Test digest first batch Carb-reporter vectors.
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<LI> Digest RBS-Carb.
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<LI> Digest, gel electrophoresis, gel purify, ligate on Carb-back bone, and transform 5 reporter vectors from the second batch.
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<LI> Overnight Ligation for third batch of reporters and promoters
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Todays Task
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      <br> Rishika sitting on the table.  
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Latest revision as of 12:53, 17 October 2014

Home Team Official Profile Project Parts Modeling Notebook Safety Attributions Human Practices

Monday, August 18, 2014

Click here to edit the page

Today is a long day. We are test digesting Tra's minipreped first batch of reporter vectors on Carb backbone from this past weekend. Two clones failed so we picked 2 colonies from each failed clone plates ctxA(2) and ctxB(2) and inoculated them. We will miniprep and test digest them again tomorrow. We threw out the miniprep products and glycerol stocks for the failed one. We got some confusion with the gel because Tra loaded multiple wells with the same sample.

We are digesting second batch of reporter vectors, and performing a 30 minute ligate on Carb backbone and then transform them. Hopefully tomorrow there will be colonies on our plates. We will pick the colonies, inoculate. The day after tomorrow we will glycerol stock them, miniprep and test digest our product. Hopefully we will have good product to complete parts of our BioBricks, (and we won't have to redo everything again) and we will keep them for later experiments.

We also digested RBS-Carb to get more Carb back bone for later use.

Lastly we did an overnight ligation for the third batch of reporters and promotors.
Today's tasks:
  • Inoculate more colonies (2) ctxA and (2) ctxB.
  • Test digest first batch Carb-reporter vectors.
  • Digest RBS-Carb.
  • Digest, gel electrophoresis, gel purify, ligate on Carb-back bone, and transform 5 reporter vectors from the second batch.
  • Overnight Ligation for third batch of reporters and promoters
Todays Task


Rishika sitting on the table.

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