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Team:Goettingen/proLP
From 2014.igem.org
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<h4><a href="https://2014.igem.org/Team:Goettingen/protocol_Plasmid_Con">Plasmid Construction</a></h4> | <h4><a href="https://2014.igem.org/Team:Goettingen/protocol_Plasmid_Con">Plasmid Construction</a></h4> | ||
<ul> | <ul> | ||
| + | <li><a href="https://2014.igem.org/Team:Goettingen/protocol_Plasmid_Con#rest_DNA">Restriction of DNA</a></li> | ||
| + | <li><a href="https://2014.igem.org/Team:Goettingen/protocol_Plasmid_Con#liga_DNA">Ligation of DNA fragments</a></li> | ||
<li><a href="https://2014.igem.org/Team:Goettingen/protocol_Plasmid_Con#BP">BP recombination reaction</a></li> | <li><a href="https://2014.igem.org/Team:Goettingen/protocol_Plasmid_Con#BP">BP recombination reaction</a></li> | ||
<li><a href="https://2014.igem.org/Team:Goettingen/protocol_Plasmid_Con#LR">LR recombination reaction</a></li> | <li><a href="https://2014.igem.org/Team:Goettingen/protocol_Plasmid_Con#LR">LR recombination reaction</a></li> | ||
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<li><a href="https://2014.igem.org/Team:Goettingen/protocol_Colony#Y2H_robot">Y2H Assay with Robot</a></li> | <li><a href="https://2014.igem.org/Team:Goettingen/protocol_Colony#Y2H_robot">Y2H Assay with Robot</a></li> | ||
</ul> | </ul> | ||
| + | <h4><a href="https://2014.igem.org/Team:Goettingen/protocol_protein">Protein Assessment</a></li> | ||
| + | <ul> | ||
| + | <li><a href="https://2014.igem.org/Team:Goettingen/protocol_protein#SDS">SDS PAGE</a></li> | ||
| + | <li><a href="https://2014.igem.org/Team:Goettingen/protocol_protein#Coo">Coomassie Staining</a></li> | ||
| + | <li><a href="https://2014.igem.org/Team:Goettingen/protocol_protein#P_Exp">Protein Expression</a></li> | ||
| + | <li><a href="https://2014.igem.org/Team:Goettingen/protocol_protein#Puri">Protein Purification</a></li> | ||
| + | <li><a href="https://2014.igem.org/Team:Goettingen/protocol_protein#Dialysis">Protein Dialysis </a></li> | ||
| + | <li><a href="https://2014.igem.org/Team:Goettingen/protocol_protein#Bradford">Bradford Assay</a></li> | ||
| + | </ul> | ||
<h4><a href="https://2014.igem.org/Team:Goettingen/protocol_protein">Protein Assessment</a></li> | <h4><a href="https://2014.igem.org/Team:Goettingen/protocol_protein">Protein Assessment</a></li> | ||
<ul> | <ul> | ||
Revision as of 12:50, 17 October 2014
Overview
PCR Methods
Plasmid Construction
- Restriction of DNA
- Ligation of DNA fragments
- BP recombination reaction
- LR recombination reaction
- SEAMLESS Cloning
- Peptide Library construction
Plasmid Transformation
- E.coli competent cells
- Plasmid isolation (E.coli)
- E.coil transformation
- Plasmid isolation (Yeast)
- Yeast transformation
