"
Team:Evry/Notebook/Transformation/25-07-2014
From 2014.igem.org
(Difference between revisions)
| (One intermediate revision not shown) | |||
| Line 10: | Line 10: | ||
<u> <b> <big><FONT COLOR=#003333>Construction of a new plasmid - Choice and amplification of the ORI</font></big></b> </u> <br> | <u> <b> <big><FONT COLOR=#003333>Construction of a new plasmid - Choice and amplification of the ORI</font></big></b> </u> <br> | ||
| - | Purification of these PCR products<br> | + | Purification of these PCR products.<br> |
Use “GenJEt” PCR Purification for it. The protocol is same that PCR purification except that the | Use “GenJEt” PCR Purification for it. The protocol is same that PCR purification except that the | ||
binding buffer is add (1μL for 1mg) and boil at 55°C during 10 mn.<br> | binding buffer is add (1μL for 1mg) and boil at 55°C during 10 mn.<br> | ||
After the purification, a new PCR is realized with 3μL to purificated fragments.<br> | After the purification, a new PCR is realized with 3μL to purificated fragments.<br> | ||
| - | Send to | + | Send to sequencing. <br> |
| + | <br> | ||
Latest revision as of 12:43, 17 October 2014
Construction of a new plasmid - Choice and amplification of the ORI
Purification of these PCR products.
Use “GenJEt” PCR Purification for it. The protocol is same that PCR purification except that the
binding buffer is add (1μL for 1mg) and boil at 55°C during 10 mn.
After the purification, a new PCR is realized with 3μL to purificated fragments.
Send to sequencing.
