Team:Evry/Notebook/Transformation/23-07-2014

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Revision as of 12:40, 17 October 2014

Picture

Construction of a new plasmid - Choice and amplification of the ORI
PCR with

  • primers 49 and 50 (see primers table in Protocols)
  • Q5 high fidelity enzyme
  • TM=60°C.

    Migration of the PCR products, we have two bands per well.
    Purification of these PCR products (no sequencage)

    July 23

    • Retrieved from "http://2014.igem.org/Team:Evry/Notebook/Transformation/23-07-2014"