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Team:Evry/Notebook/Transformation/18-07-2014
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| - | <u> <b> <big><FONT COLOR=#003333>Construction of a new plasmid</font></big></b> </u> <br> | + | <u> <b> <big><FONT COLOR=#003333>Construction of a new plasmid - Choice and amplification of the promoter</font></big></b> </u> <br> |
We chose to amplify the tkt promoter of the transkelotase gene. We find this strong constitutive promoter with the genome browser on the genome of <i>Pseudovibrio FOBEG1</i>.<br> | We chose to amplify the tkt promoter of the transkelotase gene. We find this strong constitutive promoter with the genome browser on the genome of <i>Pseudovibrio FOBEG1</i>.<br> | ||
| - | PCR with primers 5 and 6 (see primers table in <a href="https://2014.igem.org/Team:Evry/Notebook/Protocols">Protocols</a>). | + | PCR with primers 5 and 6 (see primers table in <a href="https://2014.igem.org/Team:Evry/Notebook/Protocols">Protocols</a>), Q5 high fidelity enzyme and TM=55°C. |
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Latest revision as of 12:40, 17 October 2014
Construction of a new plasmid - Choice and amplification of the promoter
We chose to amplify the tkt promoter of the transkelotase gene. We find this strong constitutive promoter with the genome browser on the genome of Pseudovibrio FOBEG1.
PCR with primers 5 and 6 (see primers table in Protocols), Q5 high fidelity enzyme and TM=55°C.
