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Team:Paris Saclay/Notebook/July/11
From 2014.igem.org
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(→Friday 11th July) |
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| Line 18: | Line 18: | ||
BioBricks used: | BioBricks used: | ||
| - | *J23100 (Amp R) | + | *J23100 (Amp R)(Dilutions: Concentrated, 0 and 10^-1) |
| - | *J23106 (Amp R) | + | *J23106 (Amp R)(Dilutions: Concentrated, 0 and 10^-1) |
| - | *J23114 (Amp R) | + | *J23114 (Amp R)(Dilutions: Concentrated, 0 and 10^-1) |
| - | + pET28a (Kan R) (pET28a is a plasmid used to verify that the 4 strains are competent). | + | + pET28a (Kan R)(Dilutions: 0, 10^-1 and 10^-2) |
| + | (pET28a is a plasmid used to verify that the 4 strains are competent). | ||
The negative controles: | The negative controles: | ||
| Line 29: | Line 30: | ||
Results: | Results: | ||
*pET28a: Nothing grows with the strains DH5α, MG1655, MG1655Z1. But the strain DH5α+ grows well. | *pET28a: Nothing grows with the strains DH5α, MG1655, MG1655Z1. But the strain DH5α+ grows well. | ||
| - | *J23100, J23106, J23114: The strain DH5α+ grow well and the 3 others strains grow less. | + | *J23100, J23106, J23114: The strain DH5α+ grow well and the 3 others strains grow less. They have to be more concentrated. |
[https://2014.igem.org/Team:Paris_Saclay/Protocols/Transformation_of_supercompetent_Ecoli_cells_with_CaCl2 Protocol] | [https://2014.igem.org/Team:Paris_Saclay/Protocols/Transformation_of_supercompetent_Ecoli_cells_with_CaCl2 Protocol] | ||
[https://2014.igem.org/Team:Paris_Saclay/Notebook Back to the calendar] | [https://2014.igem.org/Team:Paris_Saclay/Notebook Back to the calendar] | ||
Revision as of 14:00, 12 July 2014
Contents |
Friday 11th July
Lab work
1 - Fill in the WIKI (Notebook and Protocols)
Juliette & Romain
2 - Transformation of competent cells E.coli
Fabio & Maher
Competent strains used:
- DH5α
- MG1655
- MG1655Z1
- DH5α+ (given by Solenne)
BioBricks used:
- J23100 (Amp R)(Dilutions: Concentrated, 0 and 10^-1)
- J23106 (Amp R)(Dilutions: Concentrated, 0 and 10^-1)
- J23114 (Amp R)(Dilutions: Concentrated, 0 and 10^-1)
+ pET28a (Kan R)(Dilutions: 0, 10^-1 and 10^-2) (pET28a is a plasmid used to verify that the 4 strains are competent).
The negative controles:
- pET28a: without plasmid, nothing grows.
- J23100, J23106, J23114: without the BioBrick, nothing brows.
Results:
- pET28a: Nothing grows with the strains DH5α, MG1655, MG1655Z1. But the strain DH5α+ grows well.
- J23100, J23106, J23114: The strain DH5α+ grow well and the 3 others strains grow less. They have to be more concentrated.
