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Team:Paris Saclay/Notebook/July/11
From 2014.igem.org
(Difference between revisions)
(→2 - Transformation of competent cells E.coli) |
(→Friday 11th July) |
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*DH5α+ (given by Solenne) | *DH5α+ (given by Solenne) | ||
| - | + | BioBricks used: | |
*J23100 (Amp R) | *J23100 (Amp R) | ||
*J23106 (Amp R) | *J23106 (Amp R) | ||
*J23114 (Amp R) | *J23114 (Amp R) | ||
| - | + pET28a (Kan R) | + | + pET28a (Kan R) (pET28a is a plasmid used to verify that the 4 strains are competent). |
| + | |||
| + | For the 3 BB: | ||
| + | Negative control: without plasmid or BB. | ||
[https://2014.igem.org/Team:Paris_Saclay/Protocols/Transformation_of_supercompetent_Ecoli_cells_with_CaCl2 Protocol] | [https://2014.igem.org/Team:Paris_Saclay/Protocols/Transformation_of_supercompetent_Ecoli_cells_with_CaCl2 Protocol] | ||
[https://2014.igem.org/Team:Paris_Saclay/Notebook Back to the calendar] | [https://2014.igem.org/Team:Paris_Saclay/Notebook Back to the calendar] | ||
Revision as of 12:02, 12 July 2014
Contents |
Friday 11th July
Lab work
1 - Fill in the WIKI (Notebook and Protocols)
Juliette & Romain
2 - Transformation of competent cells E.coli
Fabio & Maher
Competent strains used:
- DH5α
- MG1655
- MG1655Z1
- DH5α+ (given by Solenne)
BioBricks used:
- J23100 (Amp R)
- J23106 (Amp R)
- J23114 (Amp R)
+ pET28a (Kan R) (pET28a is a plasmid used to verify that the 4 strains are competent).
For the 3 BB:
Negative control: without plasmid or BB.
