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| | <tr style="background-color:#CCD6EA; "><td colspan="2"> | | <tr style="background-color:#CCD6EA; "><td colspan="2"> |
| | <p class="title"><font color="#002B9B"> | | <p class="title"><font color="#002B9B"> |
| - | Results | + | $Results$ |
| | </font></p> | | </font></p> |
| | </td></tr> | | </td></tr> |
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| | + | <section style="margin: -40px"></section> |
| | <section style="text-align: justify; margin: 50px"> | | <section style="text-align: justify; margin: 50px"> |
| - | <h1>Characterization of our biobrick ccdB</h1>
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| - | <h2> X. 01/09/14-07/09/14 </h2>
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| - | We finished with a killing ccdB test for the characterization of pSB_1C3-ccdB: this is ok :)</p>
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| - | In the two following screens (the first is with presence of glucose, the second with arabinose), the first line corresponds to $\EColi$ without Tox-plasmid and without A-Tox plasmid (no plasmid),
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| - | the second to $\EColi$ with Tox-plasmid but without A-Tox plasmid (pBAD33::ccdB), the third to $\EColi$ without Tox-plasmid but with A-Tox plasmid (pKK233::ccdA), and the last to $\EColi$ with the two plasmids (pKK233::ccdA & pBAD33::ccdB).
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| - | The columns vary by a dilution factor (from left to right: $10^{0}$, $10^{-2}$, $10^{-3}$, $10^{-4}$, $10^{-6}$).</p>
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| - | $E.Coli \hspace{0.2cm}+\hspace{0.2cm} glucose:\hspace{1cm}$
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| - | <img src="https://static.igem.org/mediawiki/2014/d/dd/Test-ccd-iGEM-2-%2B.png"></p>
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| - | $E.Coli \hspace{0.2cm}+\hspace{0.2cm} arabinose:\hspace{0.65cm}$
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| - | <img src="https://static.igem.org/mediawiki/2014/1/11/Test-ccd-iGEM-3-%2B.png"></p>
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| - | <!--
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| - | <img src="https://static.igem.org/mediawiki/2014/a/a8/Test-ccd-iGEM-1-%2B.png"></p>
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| - | <img src="https://static.igem.org/mediawiki/2014/0/04/Test-ccd-iGEM-4-%2B.png"></p>
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| - | -->
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| - | <p>With these two killing assay screens, we see that the glucose represses the toxin (line two: bacteria survive)</p>
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| - | and arabinose inducts the production of toxin, and bacteria death.</p>
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| - | <!-- <h1>Comparison with Modelling </h1> -->
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| - | </section>
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| | </td> | | </td> |
$~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
\newcommand{\MyColi}{{\small Mighty\hspace{0.12cm}Coli}}
\newcommand{\Stabi}{\small Stabi}$
$\newcommand{\EColi}{\small E.coli}
\newcommand{\SCere}{\small S.cerevisae}\\[0cm]
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
\newcommand{\PI}{\small PI}$
$\newcommand{\Igo}{\Large\mathcal{I}}
\newcommand{\Tgo}{\Large\mathcal{T}}
\newcommand{\Ogo}{\Large\mathcal{O}}
~$
"
- Université Libre de Bruxelles -
