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Team:BYU Provo/Notebook/CRISPR/julyaug
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<p><u>7/09/2014 - Garrett Jensen.</u> | <p><u>7/09/2014 - Garrett Jensen.</u> | ||
<br/>- Today I ran a PCR on the DNA that Skip isolated from S. thermophilus on June 26 using the universal 16S ribosomal primers. I used taq polymerase instead of Redtaq because the redtaq has been having some issues lately. If there is microbial DNA present then we may be able to get PCR for the CRISPR to work from it. In the meantime I am waiting for our M17 media to show up. | <br/>- Today I ran a PCR on the DNA that Skip isolated from S. thermophilus on June 26 using the universal 16S ribosomal primers. I used taq polymerase instead of Redtaq because the redtaq has been having some issues lately. If there is microbial DNA present then we may be able to get PCR for the CRISPR to work from it. In the meantime I am waiting for our M17 media to show up. | ||
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| + | <p><u>7/10/2014 - Garrett Jensen. </u> | ||
| + | - I ran out that PCR on a gel and there was a Band!!!! That means that there is good DNA present. I started a PCR reaction using taq polymerase and our Crispr primers to see if I can get a product for the CRISPR system. If I can then I will set it up again with phusion. We still have not received the m17 media however. | ||
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Revision as of 18:55, 11 July 2014
BYU 2014 Notebook |
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7/07/2014 - Garrett Jensen.
- Today I looked around for the M17 media that supposedly was delivered 2 weeks ago. Brother Lee and Dr. Robison both said that it was not in their lab but they are the only ones who signed out a package from Fischer Scientific 2 weeks ago.
7/09/2014 - Garrett Jensen.
- Today I ran a PCR on the DNA that Skip isolated from S. thermophilus on June 26 using the universal 16S ribosomal primers. I used taq polymerase instead of Redtaq because the redtaq has been having some issues lately. If there is microbial DNA present then we may be able to get PCR for the CRISPR to work from it. In the meantime I am waiting for our M17 media to show up.
7/10/2014 - Garrett Jensen.
- I ran out that PCR on a gel and there was a Band!!!! That means that there is good DNA present. I started a PCR reaction using taq polymerase and our Crispr primers to see if I can get a product for the CRISPR system. If I can then I will set it up again with phusion. We still have not received the m17 media however.
