Team:Pitt/Skin Probiotic/Cathelicidin/Results

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<h2 id = "results">Results</h2>
<h2 id = "results">Results</h2>
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<p><b>Cathelicidin Part:</b></p>
 
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<p><br>mRFP1 Part:</b></p>
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<p><b>mRFP1 Part:</b></p>
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Revision as of 08:58, 17 October 2014

Results

mRFP1 Part:

Blue Promoter (FixK2) Part:



Lanes:              1         2         3         4       5       6       7

In Lane 1 of this gel is the 1 kb+ ladder. Lanes 3, 5, and 7 all contain the mRFP1 plasmid digested with XbaI and PstI. Each lane has two bands. The first at around ~2100 bp corresponds to the vector backbone. The second is a little above 700 bp, and corresponds to mRFP1 (770 bp).

Lanes:                      1    2    3    4    5

Lane 1 has the 1 kb+ ladder. Lanes 4 and 5 have the FixK2 part digested with XbaI and PstI. The band seen between 200 and 300 bp corresponds to FixK2 (250 bp).

Blue Sensor (YF1 and FixJ) Part:



Lanes:              1  2  3

In Lane 1 of this gel is the 1 kb+ ladder. Lanes 3, 5, and 7 all contain the mRFP1 plasmid digested with XbaI and PstI. Each lane has two bands. The first at around ~2100 bp corresponds to the vector backbone. The second is a little above 700 bp, and corresponds to mRFP1 (770 bp).

Hsp60 (no RBS) and Hsp60 (RBS) parts:



Lanes: 1         2         3         4       5       6       7

Lanes 1 and 5 have the 1 kb + ladder. Lanes 2, 3, and 4 have the Hsp60 promoter without RBS (~350 bp). Lanes 6 and 7 have the Hsp60 promoter with RBS (~380 bp).


Conclusions




Page currently under Construction.





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