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GFP＋HlyA(BBa_K1414000) and HlyA＋GFP(BBA_K1414001)

[Figure 1]

GFP

We used "BBa_E0040". Length of "BBa_E0040"is 920bp. Following figure 1 is result of the GFP PCR. As a result,We were able to confirm a band about 920bp by GFP PCR.

[Figure 2]

HlyA

We used "BBa_K554002". Length of "BBa_K554002" is 186bp. Following figure 2 is result of the HlyA PCR. As a result,We were able to confirm a band about 186bp by HlyA PCR.

[Figure 3]

[Figure 4]

GFP＋HlyA and HlyA＋GFP

GFP ligated with HlyA.Length of GFP＋HlyA is 920bp
Following figure 3 is GFP＋HlyA.
Following figure 4 is HlyA＋GFP
We submitted these parts.
But the function of these parts,we were not able to confirm it.
Because we did not have time to experiment.

@@ Line 82: / Line 82: @@
 =='''<font size="5">HlyA</font>'''==
 <font size=4>
-We used "BBa_K554002".<br>
+We used "BBa_K554002".
-Length of "BBa_K554002" is 186bp.<br>
+Length of "BBa_K554002" is 186bp.
-Following figure 2 is result of the HlyA PCR.<br>
+Following figure 2 is result of the HlyA PCR.
 As a result,We were able to confirm a band about 186bp by HlyA PCR.
 </font>

Team:KAIT Japan/Results

From 2014.igem.org

Revision as of 08:41, 17 October 2014

Contents

Results

GFP＋HlyA(BBa_K1414000) and HlyA＋GFP(BBA_K1414001)

GFP

HlyA

GFP＋HlyA and HlyA＋GFP

Creating parts of STAT3

Creating parts of IL-10α,IL-10β

Creating parts of HRV,IL-5Ra,IL-5Rb,AraC,Arac Promoter

GFP