Team:Groningen/Template/MODULE/Notebook/secretion/week3

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The RBS, Double Terminators, and the promotor LAS Biobricks were already transformed and isolated.
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The clones were inoculated in liquid media were they would be prepared for mini-prep.
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The O/N culture was mini-prepped and checked on gel.
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Revision as of 08:30, 17 October 2014

6 - 8 of August
 
Goal: obtain all the biobricks necessary for the secreting systems of P. aeruginosa and S. aureus.
 
E. coli was chemically transformed with 3 biobricks  in order to obtain them:
 
BBa_I746104: P2-promotor
BBa_K081009: LasI (for the Detection part)
BBa_C0060: Aiia
 
 
After which 1, 10, 20, 50% was inoculated on Chloramphenicol LB-agar plates.
 
The RBS, Double Terminators, and the promotor LAS Biobricks were already transformed and isolated.
 
The clones were inoculated in liquid media were they would be prepared for mini-prep.
 
The O/N culture was mini-prepped and checked on gel.
 
 
 
 

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