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Team:Groningen/Template/MODULE/Notebook/bandage/week3
From 2014.igem.org
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Before we continue with all the growth experiments we first want to check what percentage of gel works the best for our purposes. | Before we continue with all the growth experiments we first want to check what percentage of gel works the best for our purposes. | ||
We will start the experiments with these percentages: 10 - 15 - 20 - 25 and 30 % acrylamid. | We will start the experiments with these percentages: 10 - 15 - 20 - 25 and 30 % acrylamid. | ||
| + | For our purposes the 20% acrylamid gel works best, it doesn't break so fast and isn't that hard. | ||
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| + | <div class="hspacer"> </div> | ||
| + | <!-- PARAGRAPH SNIPPET END--> | ||
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| + | <!-- PARAGRAPH SNIPPET START--> | ||
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| + | After we decided the 20% gel works best we've decided to continue with the freeze drying and encapsulation of cells. | ||
| + | We've created 1 large 20% acrylamid gel which contains 1 ml of overnight culture. | ||
| + | This gel is split up 4 ways. 2 of those we're freeze dried, and the other 2 we're put in the stove overnight (30 °C). | ||
| + | |||
| + | These gels were checked on a phase contrast microscope the next day. It was clearly there were a lot of cells and that they were moving. We'll continue these experiments as soon as we have a GFP strain. | ||
</div> | </div> | ||
Revision as of 08:26, 17 October 2014
21 July - 25 July
Growing new overnight cultures and creating some chemicals for the first attempt of pouring some cells in a Gel.
Creating our first polyacrylamide Hydrogel with Lactococcus lactis in it. hoping for growth and survival.
Before we continue with all the growth experiments we first want to check what percentage of gel works the best for our purposes.
We will start the experiments with these percentages: 10 - 15 - 20 - 25 and 30 % acrylamid.
For our purposes the 20% acrylamid gel works best, it doesn't break so fast and isn't that hard.
After we decided the 20% gel works best we've decided to continue with the freeze drying and encapsulation of cells.
We've created 1 large 20% acrylamid gel which contains 1 ml of overnight culture.
This gel is split up 4 ways. 2 of those we're freeze dried, and the other 2 we're put in the stove overnight (30 °C).
These gels were checked on a phase contrast microscope the next day. It was clearly there were a lot of cells and that they were moving. We'll continue these experiments as soon as we have a GFP strain.
