Team:Pitt/Skin Probiotic/Cathelicidin/Results

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<p style = "border: 2px solid black;">In Lane 1 of this gel is the 1 kb+ ladder. Lanes 3, 5, and 7 all contain the mRFP1 plasmid digested with XbaI and PstI. Each lane has two bands. The first at around ~2100 bp corresponds to the vector backbone. The second is a little above 700 bp, and corresponds to mRFP1 (770 bp).</p></td>
<p style = "border: 2px solid black;">In Lane 1 of this gel is the 1 kb+ ladder. Lanes 3, 5, and 7 all contain the mRFP1 plasmid digested with XbaI and PstI. Each lane has two bands. The first at around ~2100 bp corresponds to the vector backbone. The second is a little above 700 bp, and corresponds to mRFP1 (770 bp).</p></td>
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<td>Lanes:&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;1&#160;&#160;&#160;&#160;&#160;&#160;&#160;2&#160;&#160;&#160;&#160;&#160;&#160;&#160;3&#160;&#160;&#160;&#160;&#160;&#160;&#160;4&#160;&#160;&#160;&#160;&#160;5<br>
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<img src = "https://static.igem.org/mediawiki/2014/2/22/Pitt_cath_results2.jpg" style = "width:2.44in;height:auto;"></td>
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<p style = "border: 2px solid black;">Lane 1 has the 1 kb+ ladder. Lanes 4 and 5 have the FixK2 part digested with XbaI and PstI. The band seen between 200 and 300 bp corresponds to FixK2 (250 bp).</p></td>
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Revision as of 08:20, 17 October 2014

Results

Cathelicidin Part:





mRFP1 Part:

Lanes:              1         2         3         4       5       6       7

In Lane 1 of this gel is the 1 kb+ ladder. Lanes 3, 5, and 7 all contain the mRFP1 plasmid digested with XbaI and PstI. Each lane has two bands. The first at around ~2100 bp corresponds to the vector backbone. The second is a little above 700 bp, and corresponds to mRFP1 (770 bp).

Lanes:         1       2       3       4     5

Lane 1 has the 1 kb+ ladder. Lanes 4 and 5 have the FixK2 part digested with XbaI and PstI. The band seen between 200 and 300 bp corresponds to FixK2 (250 bp).


Conclusions




Page currently under Construction.





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