"
Team:Paris Saclay/Notebook/June/30
From 2014.igem.org
(Difference between revisions)
(→Monday 30th June) |
(→Monday 30th June) |
||
| Line 11: | Line 11: | ||
1mL of an overnight culture in 100mL of LB | 1mL of an overnight culture in 100mL of LB | ||
| + | |||
====2 - Réhydration of selected biobricks:==== | ====2 - Réhydration of selected biobricks:==== | ||
| Line 26: | Line 27: | ||
* BBQ_J45014 | * BBQ_J45014 | ||
* BBQ_K517003 | * BBQ_K517003 | ||
| + | |||
====3 - Transformations :==== | ====3 - Transformations :==== | ||
| Line 33: | Line 35: | ||
We used the DH5α provided by our advisors for the aforementioned Biobricks. | We used the DH5α provided by our advisors for the aforementioned Biobricks. | ||
Spread on petri dishes with the concentration 10^-1 and 10^-2. | Spread on petri dishes with the concentration 10^-1 and 10^-2. | ||
| + | |||
====4 - Preparations:==== | ====4 - Preparations:==== | ||
Revision as of 09:17, 11 July 2014
Contents |
Monday 30th June
Lab work
1 - Culture or the supercompetents:
Mathias
- MG1655
- MG1655Z1
1mL of an overnight culture in 100mL of LB
2 - Réhydration of selected biobricks:
Juliette
- BBQ_I9026
- BBQ_K592025
- BBQ_K786002
- BBQ_K786003
- BBQ_K778002
- BBQ_K592011 {2 times}
- BBQ_K592009
- BBQ_K1033910 {idem}
- BBQ_J45014
- BBQ_K517003
3 - Transformations :
Maher & Mathias
We used the DH5α provided by our advisors for the aforementioned Biobricks. Spread on petri dishes with the concentration 10^-1 and 10^-2.
4 - Preparations:
Marie, Mathieu & Romain
- Aliquots of antibiotics
- Petri dishes
People there:
- Instructors : Solenne.
- Students : Arnaud, Juliette, Maher, Marie, Mathias, Mathieu, Pierre, Romain.
