Team:UT-Dallas/Notebook/8-08

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<h1 class="firstHeading" align="center">Friday, August 08, 2014</h1>
<h1 class="firstHeading" align="center">Friday, August 08, 2014</h1>
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<LI> Glycerol Stock of P+tcpS+RBS+eYFP
<LI> Glycerol Stock of P+tcpS+RBS+eYFP
<LI> Transformation of reporter vectors for ctxA, tcpJ, tcpP, tcpS
<LI> Transformation of reporter vectors for ctxA, tcpJ, tcpP, tcpS
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         <LI> Test digested gRNA vectors with 2% gel for i5.22.5,8,21,12,3,24.
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         <LI> Test digested gRNA vectors with 2% gel for i5.22.5,i5.22.8,i5.22.21,i5.22.12,i5.22.3,i5.22.24.
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<LI> Test digested tcpS reporter vectors.
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       <img src="https://static.igem.org/mediawiki/2014/thumb/d/d5/UTDiGEM_Test_digest_August_8_2014.JPG/700px-UTDiGEM_Test_digest_August_8_2014.JPG" height="400px">
       <img src="https://static.igem.org/mediawiki/2014/thumb/d/d5/UTDiGEM_Test_digest_August_8_2014.JPG/700px-UTDiGEM_Test_digest_August_8_2014.JPG" height="400px">
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       <br> Our weird fish bone gel test using 2 log ladder.
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       <br> Our weird fish bone gel test. Look at the 2 log ladder!
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       <img src="<---------image from today----------->" style="border-width: 0px; display: inline; margin-left: 0px; margin-top: 0px;" height="400px">
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       <img src="https://static.igem.org/mediawiki/2014/thumb/4/40/UTDiGEM_August8_Gel.JPG/800px-UTDiGEM_August8_Gel.JPG" style="border-width: 0px; display: inline; margin-left: 0px; margin-top: 0px;" height="400px">
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  <br> Description of image from today
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  <br> This is the nice 2% gel with 50bp ladder.
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Latest revision as of 06:34, 17 October 2014

Home Team Official Profile Project Parts Modeling Notebook Safety Attributions Human Practices

Friday, August 08, 2014

Click here to edit the page

We have a set of reporter vectors that we grew and harvested the DNA last week. Today, to qualitatively check that we got what we want, we are going to test digest our reporter vectors. After the check, we will send our second batch of sequencing order.
Today's tasks:
  • Glycerol Stock of P+tcpS+RBS+eYFP
  • Transformation of reporter vectors for ctxA, tcpJ, tcpP, tcpS
  • Test digested gRNA vectors with 2% gel for i5.22.5,i5.22.8,i5.22.21,i5.22.12,i5.22.3,i5.22.24.
  • Test digested tcpS reporter vectors.


Our weird fish bone gel test. Look at the 2 log ladder!


This is the nice 2% gel with 50bp ladder.

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