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| + | <!-- Created by Missouri Miners iGEM 2014 https://2014.igem.org/Team:Missouri_Miners CC BY-SA 3.0 --> |
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- | <style type="text/css"> | + | <link rel="stylesheet" href="https://2014.igem.org/Template:Team:Missouri_Miners/CSS?action=raw&ctype=text/css" type="text/css" /> |
- | #groupparts {text-align: center; margin-left: auto; margin-right: auto;}
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| + | <title>Team:Missouri Miners - 2014.igem.org</title> |
- | <!--main content -->
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- | <table width="70%" align="center">
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- | | + | <meta name="description" content="iGEM Missouri Miners 2014: Fixing nitrogen nitrogen oxide pollutants in coal flue gases using synthetic biology" /> |
- | | + | <meta http-equiv="content-type" content="text/html; charset=utf-8" /> |
- | <!--welcome box --> | + | <meta name="viewport" content="width=device-width" /> |
- | <tr> | + | </head> |
- | <td style="border:1px solid black;" colspan="3" align="center" height="150px" bgColor=#FF404B>
| + | <body> |
- | <h1 >WELCOME TO iGEM 2014! </h1>
| + | <div id="logo"><img src="https://static.igem.org/mediawiki/2014/2/22/Missouri_Miners_logo.png" alt="Missouri Miners iGEM" /></div> |
- | <p>Your team has been approved and you are ready to start the iGEM season! | + | <div id="menu"> |
- | <br>On this page you can document your project, introduce your team members, document your progress <br> and share your iGEM experience with the rest of the world! </p>
| + | <div id="igem-logo"> |
- | <br>
| + | <a href="https://2014.igem.org/Main_Page"><img src="https://static.igem.org/mediawiki/2014/7/74/Missouri_Miners_igem-logo.png" alt="iGEM 2014 Home" /></a> |
- | <p style="color:#E7E7E7"> <a href="https://2014.igem.org/wiki/index.php?title=Team:Missouri_Miners/Parts&action=edit"style="color:#FFFFFF"> Click here to edit this page!</a> </p>
| + | </div> |
- | </td> | + | <a href="https://2014.igem.org/Team:Missouri_Miners">Home</a> |
- | </tr>
| + | <a href="https://2014.igem.org/Team:Missouri_Miners/About">About</a> |
- | | + | <a href="https://2014.igem.org/Team:Missouri_Miners/Project">Project</a> |
- | <tr> <td colspan="3" height="5px"> </td></tr> | + | <a href="https://2014.igem.org/Team:Missouri_Miners/Parts">Parts</a> |
- | <!-- end welcome box --> | + | <label /> |
- | <tr>
| + | <a href="https://2014.igem.org/Team:Missouri_Miners/Team">Team</a> |
- | | + | <a href="https://2014.igem.org/Team:Missouri_Miners/Notebook">Notebook</a> |
- | <!--navigation menu -->
| + | <a href="https://2014.igem.org/Team:Missouri_Miners/Safety">Safety</a> |
- | <td align="center" colspan="3">
| + | <a href="https://2014.igem.org/Team:Missouri_Miners/Outreach">Outreach</a> |
- | | + | </div> |
- | <table width="100%"> | + | <div id="page"> |
- | <tr heigth="15px"></tr>
| + | <h1>Parts</h1> |
- | <tr heigth="75px"> | + | <p><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1370000" style="color:blue">Part BBa_K1370000</a> is the isolated <i>hmp</i> gene from <i>Escherichia Coli</i>, and produces a protein, dihydropteridine reductase, which catalyzes the reduction of nitric oxide to nitrate. Synthesis of its product is elevated in response to nitrite or nitric oxide through transcriptional regulation.</p> |
- | | + | <br /> |
- | | + | <div id="references"> |
- | <td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7> | + | <p>Cruz-Ramos, H., Crack, J., Wu, G., Hughes, M. N., Scott, C., Thomson, A. J., ... & Poole, R. K. (2002). NO sensing by FNR: regulation of the Escherichia coli NO-detoxifying flavohaemoglobin, Hmp. The EMBO journal, 21(13), 3235-3244.</p> |
- | <a href="https://2014.igem.org/Team:Missouri_Miners"style="color:#000000">Home </a> </td> | + | <p>Gardner, P. R., Gardner, A. M., Martin, L. A., & Salzman, A. L. (1998). Nitric oxide dioxygenase: an enzymic function for flavohemoglobin. Proceedings of the National Academy of Sciences, 95(18), 10378-10383.</p> |
- | | + | <p>Hausladen, A., Gow, A. J., & Stamler, J. S. (1998). Nitrosative stress: metabolic pathway involving the flavohemoglobin. Proceedings of the National Academy of Sciences, 95(24), 14100-14105.</p> |
- | <td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7> | + | <p>Hmp fused nitric oxide dioxygenase/dihydropteridine reductase 2. (n.d.). Retrieved from http://www.ncbi.nlm.nih.gov/gene/947018</p> |
- | <a href="https://2014.igem.org/Team:Missouri_Miners/Team"style="color:#000000"> Team </a> </td> | + | </div> |
- | | + | </div> |
- | <td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
| + | </body> |
- | <a href="https://igem.org/Team.cgi?year=2014&team_name=Missouri_Miners"style="color:#000000"> Official Team Profile </a></td>
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- | <td style="border:1px solid black" align="center" height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
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- | <a href="https://2014.igem.org/Team:Missouri_Miners/Project"style="color:#000000"> Project</a></td> | + | |
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- | <td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
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- | <a href="https://2014.igem.org/Team:Missouri_Miners/Parts"style="color:#000000"> Parts</a></td> | + | |
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- | <td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
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- | <a href="https://2014.igem.org/Team:Missouri_Miners/Modeling"style="color:#000000"> Modeling</a></td> | + | |
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- | <td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
| + | |
- | <a href="https://2014.igem.org/Team:Missouri_Miners/Notebook"style="color:#000000"> Notebook</a></td> | + | |
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- | <td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
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- | <a href="https://2014.igem.org/Team:Missouri_Miners/Safety"style=" color:#000000"> Safety </a></td> | + | |
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- | <td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
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- | <a href="https://2014.igem.org/Team:Missouri_Miners/Attributions"style="color:#000000"> Attributions </a></td> | + | |
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- | <td align ="center"> <a href="https://2014.igem.org/Main_Page"> <img src="https://static.igem.org/mediawiki/igem.org/6/60/Igemlogo_300px.png" width="55px"></a> </td>
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- | </table> | + | |
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- | <!--end navigation menu -->
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- | </td>
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- | <tr> <td colspan="3" height="15px"> </td></tr>
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- | <tr><td bgColor="#e7e7e7" colspan="3" height="1px"> </tr> | + | |
- | <tr> <td colspan="3" height="5px"> </td></tr>
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- | <!--Parts Submitted to the Registry -->
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- | <tr><td > <h3> Parts Submitted to the Registry </h3></td> | + | |
- | <td ></td >
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- | <td > <h3>What information do I need to start putting my parts on the Registry? </h3></td>
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- | </tr> | + | |
- | <tr> | + | |
- | <td width="45%" valign="top">
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- | <p> | + | |
- | An important aspect of the iGEM competition is the use and creation of standard biological parts. Each team will make new parts during iGEM and will submit them to the <a href="http://partsregistry.org"> Registry of Standard Biological Parts</a>. The iGEM software provides an easy way to present the parts your team has created. The "groupparts" tag will generate a table with all of the parts that your team adds to your team sandbox.
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- | <p>
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- | <strong>Note that if you want to document a part you need to document it on the <a href="http://partsregistry.org Registry"> Registry</a>, not on your team wiki.</strong> Future teams and other users and are much more likely to find parts on the Registry than on your team wiki.
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- | </p>
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- | <p>
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- | Remember that the goal of proper part documentation is to describe and define a part, so that it can be used without a need to refer to the primary literature. Registry users in future years should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for users who wish to know more.
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- | </p> | + | |
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- | | + | |
- | <h3>When should you put parts into the Registry?</h3>
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- | <p> | + | |
- | As soon as possible! We encourage teams to start completing documentation for their parts on the Registry as soon as you have it available. The sooner you put up your parts, the better recall you will have of all details surrounding your parts. Remember you don't need to send us the DNA to create an entry for a part on the Registry. However, you must send us the sample/DNA before the Jamboree. Only parts for which you have sent us samples/DNA are eligible for awards and medal requirements.
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- | </p>
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- | <td > </td>
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- | <p>
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- | The information needed to initially create a part on the Registry is:
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- | </p>
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- | <ol>
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- | <li>Part Name</li>
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- | <li>Part type</li>
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- | <li>Creator</li>
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- | <li>Sequence</li>
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- | <li>Short Description (60 characters on what the DNA does)</li>
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- | <li>Long Description (Longer description of what the DNA does)</li>
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- | <li>Design considerations</li>
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- | </ol>
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- | <p> | + | |
- | We encourage you to put up <em>much more</em> information as you gather it over the summer. If you have images, plots, characterization data and other information, please also put it up on the part page. Check out part <a href="http://parts.igem.org/Part:BBa_K404003">BBa_K404003</a> for an excellent example of a highly characterized part.
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- | </p> | + | |
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- | <p> | + | |
- | You can add parts to the Registry at our <a href="http://parts.igem.org/Add_a_Part_to_the_Registry"> Add a Part to the Registry</a> link.
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- | </p> | + | |
- | </td> | + | |
- | </tr> | + | |
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- | <tr> <td colspan="3" height="15px"> </td></tr>
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- | <tr><td colspan="3" > <h3> Parts Table</h3></td></tr>
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- | <tr><td width="45%" colspan="3" valign="top">
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- | Any parts your team has created will appear in this table below:</td></tr>
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- | </table> | + | |
| </html> | | </html> |
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| |
- | <groupparts>iGEM013 Missouri_Miners</groupparts>
| |
Parts
Part BBa_K1370000 is the isolated hmp gene from Escherichia Coli, and produces a protein, dihydropteridine reductase, which catalyzes the reduction of nitric oxide to nitrate. Synthesis of its product is elevated in response to nitrite or nitric oxide through transcriptional regulation.
Cruz-Ramos, H., Crack, J., Wu, G., Hughes, M. N., Scott, C., Thomson, A. J., ... & Poole, R. K. (2002). NO sensing by FNR: regulation of the Escherichia coli NO-detoxifying flavohaemoglobin, Hmp. The EMBO journal, 21(13), 3235-3244.
Gardner, P. R., Gardner, A. M., Martin, L. A., & Salzman, A. L. (1998). Nitric oxide dioxygenase: an enzymic function for flavohemoglobin. Proceedings of the National Academy of Sciences, 95(18), 10378-10383.
Hausladen, A., Gow, A. J., & Stamler, J. S. (1998). Nitrosative stress: metabolic pathway involving the flavohemoglobin. Proceedings of the National Academy of Sciences, 95(24), 14100-14105.
Hmp fused nitric oxide dioxygenase/dihydropteridine reductase 2. (n.d.). Retrieved from http://www.ncbi.nlm.nih.gov/gene/947018