Template:Nevada/Main2

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                             <p>This summer, the iGEM team of Nevada came up with a new strategy for rapid response protein degradation in yeast cells through our <u>B</u>ioorthoginal <u>A</u>uxin <u>I</u>nducible <u>T</u>rigger <u>Switch</u> (or BAITSwitch). With usual protein degradation being a very slow process which can take several hours, or even days. And the perfect system being similar to that of a light switch; where a response is seen immediately after a trigger is toggled.
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                             <p>This summer the Nevada iGEM team used (and learned!) many protocols involving cloning techniques, working with yeast, growth assays, etc. We hope that other teams can benefit from our experiences. </p>
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                            This year we attempted to select a specifically labeled protein for immediate degradation via the Bioorthoginal auxin-inducible-degron (AID) System (AUTHOR GOES HERE). This Auxinsystem has been published and used by previous iGEM teams, but has never been combined with its parallel system, jasmonic acid, in non-plant cells. However, before we could start our journey we needed to understand some very unique characteristics about Protein Degradation. </p>
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<a href="https://static.igem.org/mediawiki/2014/f/f8/UNR_Primer_and_PCR_Protocols.pdf" title = "Protocols">PCR and Primer Protocols</a><br>
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<a href=" https://static.igem.org/mediawiki/2014/7/79/E_Coli_Transformation_%28post_Gibson_Assembly%29.pdf" title = "Protocols"><i>E. coli</i> Transformation</a><br>
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<a href=" https://static.igem.org/mediawiki/2014/8/8b/UNR_Yeast_Media_Dropout_Recipes_Sheet1.pdf" title = "Protocols">Yeast Media Recipes</a><br>
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<a href=" https://static.igem.org/mediawiki/2014/5/52/UNR_Western_Blot_Protocol_to_detect_GFP_in_yeast.pdf" title = "Protocols">Western Blot Protocols</a><br>
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Latest revision as of 04:09, 17 October 2014

Nevada iGEM 2014

Protocols

This summer the Nevada iGEM team used (and learned!) many protocols involving cloning techniques, working with yeast, growth assays, etc. We hope that other teams can benefit from our experiences.


PCR and Primer Protocols
E. coli Transformation
Yeast Media Recipes
Western Blot Protocols
Team Nevada

The BAITswitch

The Bioorthoginal Auxin Induceable Trigger Switch

Subscribe

Stay updated on everything happening with Team Nevada as they journey toward the iGEM Jamboree .

Our Sponsors

Here is a list of all of the people who have helped make Team Nevada's iGEM expierence possible.

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