Team:UT-Dallas/Notebook/8-11

From 2014.igem.org

(Difference between revisions)
(Created page with "{{CSS/Main}} <html xmlns="http://www.w3.org/1999/xhtml" xml:lang="en" lang="en" dir="ltr"> <h1 class="firstHeading" align="center">Sunday, August 10, 2014</h1> <center><p><a hre...")
 
(12 intermediate revisions not shown)
Line 2: Line 2:
<html xmlns="http://www.w3.org/1999/xhtml" xml:lang="en" lang="en" dir="ltr">
<html xmlns="http://www.w3.org/1999/xhtml" xml:lang="en" lang="en" dir="ltr">
-
<h1 class="firstHeading" align="center">Sunday, August 10, 2014</h1>
+
<!--navigation menu -->
-
<center><p><a href="https://2014.igem.org/wiki/index.php?title=Team:UT-Dallas/Notebook/8-10&action=edit "style="color:#87A96B" >Click here to edit the page </a> </p></center>
+
<table align="center">
 +
<td align="center" colspan="12">
 +
 
 +
<table  width="100%">
 +
<tr height="15px"></tr>
 +
<tr height="75px">
 +
 
 +
<td style="display: inline; padding: 15px; text-decoration: none; " align="center" height ="45px" onMouseOver="this.bgColor='#4E817B'" onMouseOut="this.bgColor='#70B9B0'" bgColor=#70B9B0> 
 +
<a href="https://2014.igem.org/Team:UT-Dallas"style="color:#ffffff; text-decoration: none; text-transform: uppercase; font-weight: bold;" >Home </a> </td>
 +
 
 +
<td style="display: inline; padding: 15px; text-decoration: none;" align="center" height ="45px" onMouseOver="this.bgColor='#4E817B'" onMouseOut="this.bgColor='#70B9B0'" bgColor=#70B9B0>
 +
<a href="https://2014.igem.org/Team:UT-Dallas/Team"style="color:#ffffff; text-decoration: none; text-transform: uppercase; font-weight: bold;"> Team </a> </td>
 +
 
 +
<td style="display: inline; padding: 15px; text-decoration: none;" align="center"  height ="45px"  onMouseOver="this.bgColor='#4E817B'" onMouseOut="this.bgColor='#70B9B0'" bgColor=#70B9B0>
 +
<a href="https://igem.org/Team.cgi?year=2014&team_name=UT-Dallas"style="color:#ffffff; text-decoration: none; text-transform: uppercase; font-weight: bold;"> Official Profile </a></td>
 +
 
 +
<td style="display: inline; padding: 15px; text-decoration: none;" align="center"  height ="45px" onMouseOver="this.bgColor='#4E817B'" onMouseOut="this.bgColor='#70B9B0'" bgColor=#70B9B0> 
 +
<a href="https://2014.igem.org/Team:UT-Dallas/Project"style="color:#ffffff; text-decoration: none; text-transform: uppercase; font-weight: bold;"> Project</a></td>
 +
 
 +
<td style="display: inline; padding: 15px; text-decoration: none;" align="center"  height ="45px" onMouseOver="this.bgColor='#4E817B'" onMouseOut="this.bgColor='#70B9B0'" bgColor=#70B9B0>
 +
<a href="https://2014.igem.org/Team:UT-Dallas/Parts"style="color:#ffffff; text-decoration: none; text-transform: uppercase; font-weight: bold;"> Parts</a></td>
 +
 
 +
<td style="display: inline; padding: 15px; text-decoration: none;" align="center" height ="45px" onMouseOver="this.bgColor='#4E817B'" onMouseOut="this.bgColor='#70B9B0'" bgColor=#70B9B0>
 +
<a href="https://2014.igem.org/Team:UT-Dallas/Modeling"style="color:#ffffff; text-decoration: none; text-transform: uppercase; font-weight: bold;"> Modeling</a></td>
 +
 
 +
<td style="display: inline; padding: 15px; text-decoration: none;" align="center" height ="45px" onMouseOver="this.bgColor='#4E817B'" onMouseOut="this.bgColor='#70B9B0'" bgColor=#70B9B0> 
 +
<a href="https://2014.igem.org/Team:UT-Dallas/Notebook"style="color:#ffffff; text-decoration: none; text-transform: uppercase; font-weight: bold;"> Notebook</a></td>
 +
 
 +
<td style="display: inline; padding: 15px; text-decoration: none;" align="center"  height ="45px" onMouseOver="this.bgColor='#4E817B'" onMouseOut="this.bgColor='#70B9B0'" bgColor=#70B9B0>
 +
<a href="https://2014.igem.org/Team:UT-Dallas/Safety"style=" color:#ffffff; text-decoration: none; text-transform: uppercase; font-weight: bold;"> Safety </a></td>
 +
 
 +
<td style="display: inline; padding: 15px; text-decoration: none;" align="center"  height ="45px" onMouseOver="this.bgColor='#4E817B'" onMouseOut="this.bgColor='#70B9B0'" bgColor=#70B9B0>
 +
<a href="https://2014.igem.org/Team:UT-Dallas/Attributions"style="color:#ffffff; text-decoration: none; text-transform: uppercase; font-weight: bold;"> Attributions </a></td>
 +
 
 +
<td style="display: inline; padding: 15px; text-decoration: none;" align="center"  height ="45px" onMouseOver="this.bgColor='#4E817B'" onMouseOut="this.bgColor='#70B9B0'" bgColor=#70B9B0>
 +
<a href="https://2014.igem.org/Team:UT-Dallas/Human-Practices"style="color:#ffffff; text-decoration: none; text-transform: uppercase; font-weight: bold;"> Human Practices</a></td>
 +
 
 +
 
 +
<td style="display: inline; padding: 5px;" align ="center"> <a href="https://2014.igem.org/Team:UT-Dallas"> <img src="https://static.igem.org/mediawiki/2014/e/e1/UTDmodifiedlogo.jpg" height="45px"></a></td>
 +
<td style="display: inline; padding: 5px;" align ="center"> <a href="https://2014.igem.org/Main_Page"> <img src="https://static.igem.org/mediawiki/igem.org/6/60/Igemlogo_300px.png" width="55px"></a> </td>
 +
 
 +
</tr>
 +
</table>
 +
</tr>
 +
 
 +
 
 +
</tr>
 +
 +
 
 +
</td>
 +
 
 +
<tr> <td colspan="3"  height="15px"> </td></tr>
 +
<tr><td bgColor="#e7e7e7" colspan="3" height="1px"> </tr>
 +
<tr> <td colspan="3"  height="5px"> </td></tr>
 +
</table>
 +
 
 +
<!------end nav-->
 +
 
 +
<h1 class="firstHeading" align="center">Monday, August 11, 2014</h1>
 +
<center><p><a href="https://2014.igem.org/wiki/index.php?title=Team:UT-Dallas/Notebook/8-11&action=edit "style="color:#87A96B" >Click here to edit the page </a> </p></center>
Line 11: Line 70:
  <td>  
  <td>  
  <hr width="100%" align="center">
  <hr width="100%" align="center">
-
  <p> Purified plasmid DNA from potential Reporter clones for later testing.  </p>
+
 
-
   <p> Test purified plasmid DNA for correct insert. </P>
+
<style type="text/css">
 +
.tab{text-indent:40px;}
 +
  </style>
 +
<p class="tab">
 +
   A short meeting (2 hours) to discuss our progress so far and further prospective of our project.</p>
 +
<p class="tab">
 +
  We have a set of reporter vectors that we grew and harvested the DNA last week. Today, to qualitatively check that we got what we want, we are going to test digest our reporter vectors. After the check, we will send our second batch of sequencing order.</p>
 +
<p class="tab">Kristina brought lots of food: cookies, muffins, etc. We also have a dry ice party at the end of lab day!
 +
</p>
    
    
  </td>
  </td>
Line 23: Line 90:
     <tr>
     <tr>
       <th><font size="3">Today's tasks:</font>
       <th><font size="3">Today's tasks:</font>
-
<p>Miniprep</p>
 
-
<p>Inoculated more clones</p>
 
       </th>       
       </th>       
     </tr>
     </tr>
Line 33: Line 98:
       <hr width="100%" align="center">
       <hr width="100%" align="center">
<UL>
<UL>
-
<LI> Miniprep potential transformants containing Reporter plasmids for ctxB, tcpJ, tcpP, and tcpS
+
<LI> Miniprep tcpS clones
-
         <LI> Clone inoculated from tcpS plate did not show expected yellow phenotype. 2 additional clones from the tcpS plate were inoculated in Chloramphenicol broth overnight to be purified and tested on Monday.  
+
<LI> Test digest Reporter plasmids for ctxA, tcpJ, tcpP, and tcpS
 +
         <LI> Streak M13.
 +
<LI> Ligate + transform chromoproteins [P+RBS+chromo]
 +
<LI> Set up overnight ligation for tcpD, toxT.
</UL>
</UL>
       </td>
       </td>
<td>
<td>
-
<img class="pic" src="http://i.imgur.com/61AwAAW.jpg" width="300px" align="right"/>
+
 
 +
<img class="pic" src="https://static.igem.org/mediawiki/2014/0/08/August_11_2_%282%29.jpg" width="300px" align="right"/>
</tr>
</tr>
   </tbody>
   </tbody>
</table>
</table>
 +
 +
<table width="50%" align="center">
<table width="50%" align="center">
Line 48: Line 119:
  <hr width="100%" align="center">
  <hr width="100%" align="center">
   <p>
   <p>
-
       <img src="http://i.imgur.com/wEYQB0n.jpg" height="400px">
+
       <img src="https://static.igem.org/mediawiki/2014/6/64/Megan.jpg" style="border-width: 0px; display: inline; margin-left: 0px; margin-top: 0px" height="400px">
-
       <br> Preparing cells for plasmid purification.
+
       <br> Megan Preparing cells for plasmid purification.
       <div class="gallerylayer" opacity: 1; background: white;">
       <div class="gallerylayer" opacity: 1; background: white;">
-
       <img src="<---------image from today----------->" style="border-width: 0px; display: inline; margin-left: 0px; margin-top: 0px;" height="400px">
+
       <img src="https://static.igem.org/mediawiki/2014/5/54/UTDallasAugust11.jpg" style="border-width: 0px; display: inline; margin-left: 0px; margin-top: 0px;" height="400px">
-
  <br> Description of image from today
+
  <br> Megan and tra playing with dry ice during down time
-
       <img src="<---------image from today----------->" style="border-width: 0px; display: none;" height="400px">
+
        
       </div>
       </div>

Latest revision as of 04:04, 17 October 2014

Home Team Official Profile Project Parts Modeling Notebook Safety Attributions Human Practices

Monday, August 11, 2014

Click here to edit the page

A short meeting (2 hours) to discuss our progress so far and further prospective of our project.

We have a set of reporter vectors that we grew and harvested the DNA last week. Today, to qualitatively check that we got what we want, we are going to test digest our reporter vectors. After the check, we will send our second batch of sequencing order.

Kristina brought lots of food: cookies, muffins, etc. We also have a dry ice party at the end of lab day!

Today's tasks:
  • Miniprep tcpS clones
  • Test digest Reporter plasmids for ctxA, tcpJ, tcpP, and tcpS
  • Streak M13.
  • Ligate + transform chromoproteins [P+RBS+chromo]
  • Set up overnight ligation for tcpD, toxT.


Megan Preparing cells for plasmid purification.


Megan and tra playing with dry ice during down time

Retrieved from "http://2014.igem.org/Team:UT-Dallas/Notebook/8-11"