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Revision as of 03:49, 17 October 2014
Protocols
This summer, the iGEM team of Nevada came up with a new strategy for rapid response protein degradation in yeast cells through our Bioorthoginal Auxin Inducible Trigger Switch (or BAITSwitch). With usual protein degradation being a very slow process which can take several hours, or even days. And the perfect system being similar to that of a light switch; where a response is seen immediately after a trigger is toggled. This year we attempted to select a specifically labeled protein for immediate degradation via the Bioorthoginal auxin-inducible-degron (AID) System (AUTHOR GOES HERE). This Auxinsystem has been published and used by previous iGEM teams, but has never been combined with its parallel system, jasmonic acid, in non-plant cells. However, before we could start our journey we needed to understand some very unique characteristics about Protein Degradation.