Team:Rutgers
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Revision as of 03:41, 17 October 2014
The Next Step in DNA Synthesis
Abstract
- In today's world we use organic solvents and reactive amidites to synthesize all custom DNA.
- These methods are in need of improvement. The efficiencies are too low for many of today's applications.
- It may be prudent to begin investigating more natural, aqueous environments that are chemically less harmful to the DNA during synthesis.
- An aqueous synthesis environment would allow us to use the enzymes that have evolved alongside nucleic acids for billions of years.
WHY
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HOW
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Accomplishments
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Parts
Name |
Type |
Decription |
Length |
---|---|---|---|
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coding |
Mouse TdT |
1590 |
Students
Kenny Kostenbader Chem Eng |
Scott Lazaro Cell Bio & Neurosci |
Wilson Wong Mol Bio |
Jay Patel Chem Eng |
Faculty
Sagar Khare P.I. |
Andrew Laudisi Lab Manager |
Attributions
Dr Jones is a professor in the Rutgers Chemistry Department who researches modified nucleotides. He gave valuable feedback on our initial project ideas, and suggested a way to create (and characterize) 3'-acetylated thymidine triphosphate in our lab. We attempted this synthesis (involving pyridine and acetic anhydride) and got promising results (via LC/MS), but then we found out that TriLink (below) could simply synthesize and purify it for us for free. |
Arun Nayar was on last year's Rutgers iGEM team, so he helped train us with various lab protocols. |
All of the pictured results on the Project page represent assays that were designed jointly by the students and Dr Khare, and carried out completely by the students in the lab. Additional help was provided by other Rutgers students as well, namely: Diego Barreto, Wesley Okwemba, Neil Patel, Harsh Patel, and Samantha Ashley. |
Trilink Biotech supported the project by custom-synthesizing acetylated thymidine triphosphate, and supplying it free of charge. |
NEB Inc supported the project by supplying a Terminal Transferase enzyme kit and a dNTP set, both free of charge. |
Gen9 supported the project with a $500 donation. |
Protocols
Click to download a copy of protocols that we followed and/or wrote this summer: