Team:UT-Dallas/Notebook/8-10

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<h1 class="firstHeading" align="center">Sunday, August 10, 2014</h1>
<center><p><a href="https://2014.igem.org/wiki/index.php?title=Team:UT-Dallas/Notebook/8-10&action=edit "style="color:#87A96B" >Click here to edit the page </a> </p></center>
<center><p><a href="https://2014.igem.org/wiki/index.php?title=Team:UT-Dallas/Notebook/8-10&action=edit "style="color:#87A96B" >Click here to edit the page </a> </p></center>
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<LI> Miniprep potential transformants containing Reporter plasmids for ctxB, tcpJ, tcpP, and tcpS
<LI> Miniprep potential transformants containing Reporter plasmids for ctxB, tcpJ, tcpP, and tcpS
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<UL>  <LI> Clone inoculated from tcpS plate did not show expected yellow phenotype. 2 additional clones from the tcpS plate were inoculated in Chloramphenicol broth overnight to be purified and tested on Monday.  
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        <LI> Clone inoculated from tcpS plate did not show expected yellow phenotype. 2 additional clones from the tcpS plate were inoculated in Chloramphenicol broth overnight to be purified and tested on Monday.  
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       <br> Preparing cells for plasmid purification.
       <br> Preparing cells for plasmid purification.
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Latest revision as of 03:26, 17 October 2014

Home Team Official Profile Project Parts Modeling Notebook Safety Attributions Human Practices

Sunday, August 10, 2014

Click here to edit the page

Purified plasmid DNA from potential Reporter clones for later testing.

Test purified plasmid DNA for correct insert.

Today's tasks:

Miniprep

Inoculated more clones
  • Miniprep potential transformants containing Reporter plasmids for ctxB, tcpJ, tcpP, and tcpS
  • Clone inoculated from tcpS plate did not show expected yellow phenotype. 2 additional clones from the tcpS plate were inoculated in Chloramphenicol broth overnight to be purified and tested on Monday.


Preparing cells for plasmid purification.

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