Team:ITB Indonesia/DegMod

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<li>TEAM
<li>TEAM
<ul>
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<li><a href="https://igem.org/Team.cgi?year=2014" >OFFICIAL TEAM PROFILE</a></li>
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<li><a href="https://igem.org/Team.cgi?id=1387" >OFFICIAL TEAM PROFILE</a></li>
<li><a href="https://2014.igem.org/Team:ITB_Indonesia/Team">TEAM MEMBER</a></li>
<li><a href="https://2014.igem.org/Team:ITB_Indonesia/Team">TEAM MEMBER</a></li>
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<li><a href="https://2014.igem.org/Team:ITB_Indonesia/RepMod">REPORTER MODULE</a></li>
<li><a href="https://2014.igem.org/Team:ITB_Indonesia/RepMod">REPORTER MODULE</a></li>
<li><a href="https://2014.igem.org/Team:ITB_Indonesia/SelfMod">SELF REGULATORY MODULE</a></li>
<li><a href="https://2014.igem.org/Team:ITB_Indonesia/SelfMod">SELF REGULATORY MODULE</a></li>
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<li><a href="https://2014.igem.org/Team:ITB_Indonesia/FutureSystem">FUTURE SYSTEM</a></li>
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<li>MODELING</li>
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<li><a href="https://2014.igem.org/Team:ITB_Indonesia/Modeling">MODELING</a></li>
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<li>NOTEBOOK</li>
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<li>WETLAB
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<li><a href="https://2014.igem.org/Team:ITB_Indonesia/protocol">PROTOCOL</a></li>
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<li><a href="https://2014.igem.org/Team:ITB_Indonesia/Parts">PARTS</a></li>
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<li><a href="https://2014.igem.org/Team:ITB_Indonesia/Attributions">ATTRIBUTIONS</a></li>
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<li><a href="https://2014.igem.org/Team:ITB_Indonesia/Safety">SAFETY</a></li>
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<li><a href="https://2014.igem.org/Team:ITB_Indonesia/Data">DATA</a></li>
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<li><a href="https://2014.igem.org/Team:ITB_Indonesia/ProteinModel">PROTEIN MODEL</a></li>
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<li><a href="https://2014.igem.org/Team:ITB_Indonesia/Achievement">ACHIEVEMENT</a></li>
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<li>NOTEBOOK
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<li><a href="https://2014.igem.org/Team:ITB_Indonesia/nb-wetlab">WETLAB</a></li>
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<li>HUMAN PRACTICE
<li>HUMAN PRACTICE
<ul>
<ul>
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<li><a href="https://2014.igem.org/Team:ITB_Indonesia/OnMedia">MEDIA</a></li>
<li><a href="https://2014.igem.org/Team:ITB_Indonesia/OnMedia">MEDIA</a></li>
<li><a href="https://2014.igem.org/Team:ITB_Indonesia/Biosafety">BIOSAFETY SEMINAR</a></li>
<li><a href="https://2014.igem.org/Team:ITB_Indonesia/Biosafety">BIOSAFETY SEMINAR</a></li>
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<li>ON PROGRESS</li>
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<li><a href="https://2014.igem.org/Team:ITB_Indonesia/MeetUp">INDONESIA TEAMS MEET UP</a></li>
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<li><a href="https://2014.igem.org/Team:ITB_Indonesia/Kids">FUN WITH KIDS</a></li>
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<li><a href="https://2014.igem.org/Team:ITB_Indonesia/UPI">SHARING SYNBIO IN UPI</a></li>
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<br>
<h1>Degradation Module</h1>
<h1>Degradation Module</h1>
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<p>Team ITB_Indonesia tries to make a module to degrade PET. We use LC-Cutinase fused with ompA and Lpp signal sequence. LC-Cutinase will break the ester bonds on PET structure through esterase activity of the enzyme and generate ethylene glycol and terephthalic acid as product. We plan to expose this enzyme on the surface membrane of bacteria thus making the enzyme immobile and more thermostable. On the other hand, PET which has high molecular mass can be degraded directly without need to pass through bacteria cell membrane. This molecular machine then named as whole cell biocatalyst.</p>
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<p align="justify">Team ITB_Indonesia tries to make a module to degrade PET. We use LC-Cutinase fused with ompA and Lpp signal sequence. LC-Cutinase will break the ester bonds on PET structure through esterase activity of the enzyme and generate ethylene glycol and terephthalic acid as product. We plan to expose this enzyme on the surface membrane of bacteria thus making the enzyme immobile and more thermostable. On the other hand, PET which has high molecular mass can be degraded directly without need to pass through bacteria cell membrane. This molecular machine then named as whole cell biocatalyst.</p>
<img src="https://static.igem.org/mediawiki/2014/3/30/Degradation-module.jpg">
<img src="https://static.igem.org/mediawiki/2014/3/30/Degradation-module.jpg">

Latest revision as of 03:15, 17 October 2014


Degradation Module

Team ITB_Indonesia tries to make a module to degrade PET. We use LC-Cutinase fused with ompA and Lpp signal sequence. LC-Cutinase will break the ester bonds on PET structure through esterase activity of the enzyme and generate ethylene glycol and terephthalic acid as product. We plan to expose this enzyme on the surface membrane of bacteria thus making the enzyme immobile and more thermostable. On the other hand, PET which has high molecular mass can be degraded directly without need to pass through bacteria cell membrane. This molecular machine then named as whole cell biocatalyst.



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