Team:Paris Saclay/Notebook/July/9

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*DH5αZ1 P5B1C3 RBS amilCP Ter clone 9, A.
*DH5αZ1 P5B1C3 RBS amilCP Ter clone 9, A.
*DH5αZ1 P5B1C3 RBS amilCP Ter clone 9, B.
*DH5αZ1 P5B1C3 RBS amilCP Ter clone 9, B.
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*XL1 Blue pCola-ges Kan
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*XL1-Blue pCola-ges Kan
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*PJBEI-6409 Cm
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*pJBEI-6409 Cm
[https://2013.igem.org/Team:Paris_Saclay/gel Protocol]
[https://2013.igem.org/Team:Paris_Saclay/gel Protocol]

Revision as of 08:11, 10 July 2014

Contents

Wednesday 9th July

Lab work

1 - Plasmid DNA Extraction:

Romain & Sean

Plasmids used: DH5αZ1 P5B1C3 RBS amilCP Ter clone 9, A and B.

Protocol

2 - Electrophoresis:

Arnaud & Romain & Sean

Plasmids used:

  • DH5αZ1 P5B1C3 RBS amilCP Ter clone 9, A.
  • DH5αZ1 P5B1C3 RBS amilCP Ter clone 9, B.
  • XL1-Blue pCola-ges Kan
  • pJBEI-6409 Cm

Protocol

3 - New culture:

Romain

New liquid cultures of:

  • FNR-RBS-amilCp-Ter DH5αZ1 P5B1C3 Cm
  • BBa B0015 dble ter P5B1C3 Cm

4x 5ml LB + 20μl Chloramphenicol + a little of bacteria. We incubate cultures at 37°C.

Human Pratices

Conference with the team to discuss about the project. The handled topics were:

  • Definition of Biobricks to make
  • Planning of internships
  • Definition of Biobricks to render
  • Understanding the construction of consecutive genes
  • Organisation and planning of experiences to do
  • Sponsoring
  • Wiki's progress
  • Modelling's progress


People there:

  • Instructors and advisors: Alice, Olivier, Solenne and Sylvie.
  • Students: Arnaud, Fabio, Juliette, Maher, Marie, Mathias, Mathieu, Pierre, Romain and Sean.
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