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Revision as of 22:13, 9 July 2014

BYU 2014 Notebook Edit July August

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9 July 2014

Alpha Amylase: We discovered that the box of DH5a we had been using last week was not actually DH5a as someone had put the wrong tubes in the box. Hopefully this means that the transformations are working (given that we are actually transforming it into DH5a!). The mutated alpha amylase was retransformed into DH5a using 10 uL of PCR product as suggested last time by Dr. Grose since the PCR product was diluted during DpnI digest.