Team:METU Turkey safety

From 2014.igem.org

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<h3>Degredo PETronum!</h3>
<h3>Degredo PETronum!</h3>
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<p> ABSTRACT</p>
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<p> An importat issue: Safety!</p>
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<p> The usage of polyethylene  terephthalate-PET (as like plastic bottles or textiles)  is one of the big problems of our era. Many plastic bottles are not reusable and are not safe for hot liquids or for use in dishwashers or microwaves. Some tougher, reusable plastic bottles may cause cancer. The main disadvantage of plastic bottles is that so many of them end up in landfills instead of being recycled. It takes thousands of years for plastic bottles to biodegrade, and the degrading process emits toxic chemicals into the air.
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<p> All projects are being conducted in lab-safe strains of E.coli and P.putida. All researchers have been trained in applicable lab safety to insure that no bacteria are inadvertently released into the environment. We have also been trained in proper handling of chemicals. In plastics projects, the actual organisms being engineered were maintained in lab conditions (cultures, bioreactors, etc.).
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We may alter the destiny of plastic in useful way.</p>
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None of the parts we made this year raise any particular safety issues that we can foresee. All of our major parts were either taken from pre-registered biobricks, None of our new parts would provide any foreseeable selective advantage in the wild. Thus, these parts would not increase bacterial survival in the case of accidental release.</p>
<p> OUR MISSION</p>
<p> OUR MISSION</p>
<p> Our project aims to clean enviroment with the degredation of PET to pyruvate by E.coli. With this project,while  the environment will be cleaned from PET, the E.coli would add pyruvate to the cell cycle and use it as a new source.
<p> Our project aims to clean enviroment with the degredation of PET to pyruvate by E.coli. With this project,while  the environment will be cleaned from PET, the E.coli would add pyruvate to the cell cycle and use it as a new source.

Revision as of 01:26, 17 October 2014

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Welcome!
We are Team METU Turkey!

Scroll down to find more about our project!

Degredo PETronum!

An importat issue: Safety!

All projects are being conducted in lab-safe strains of E.coli and P.putida. All researchers have been trained in applicable lab safety to insure that no bacteria are inadvertently released into the environment. We have also been trained in proper handling of chemicals. In plastics projects, the actual organisms being engineered were maintained in lab conditions (cultures, bioreactors, etc.). None of the parts we made this year raise any particular safety issues that we can foresee. All of our major parts were either taken from pre-registered biobricks, None of our new parts would provide any foreseeable selective advantage in the wild. Thus, these parts would not increase bacterial survival in the case of accidental release.

OUR MISSION

Our project aims to clean enviroment with the degredation of PET to pyruvate by E.coli. With this project,while the environment will be cleaned from PET, the E.coli would add pyruvate to the cell cycle and use it as a new source. We divide our project into two parts. At the first part we found gene design for converting the PET into 2-hydroxymuconate semialdehyde in iGEM database. The arrangement at the bottom explains the first part:

At the second part, we wanted to go further and researched the gene parts for convertion of 2-hydroxymuconate semialdehyde into pyruvate. This part will give us the chance of adding new parts to iGEM database. In this arrangement, it can be observed in detail:

How does our project work?

Totally degradation of PET to pyruvate is done by E. coli.

Who will our project help?

Our project will help the environment to become cleaner.

Why did we choose this project?

The biodegradation of plastic bottles took thousands of years, and the degrading process emits toxic chemicals into the air. Our project aims to clean enviroment with the degredation of PET to pyruvate by E.coli. With this project,while the environment will be cleaned from PET, the E.coli would add pyruvate to the cell cycle and use it as a new source.

Our Supporter: